Ignite Creation Date:
2024-05-06 @ 1:50 PM
Last Modification Date:
2024-10-26 @ 1:20 PM
Study NCT ID:
NCT04137406
Status:
UNKNOWN
Last Update Posted:
2019-10-24
First Post:
2019-10-22
Brief Title:
Role of SIRT1 in Regulation of Epithelial-to-mesenchymal Transition in Breast Cancer Lymph Nodes Metastasis
Sponsor:
Peking University Peoples Hospital
Organization:
Peking University Peoples Hospital
Study Overview
Official Title:
Role of SIRT1 in Regulation of Epithelial-to-mesenchymal Transition in Breast Cancer Lymph Nodes Metastasis for Luminal A Subtype
Status:
UNKNOWN
Status Verified Date:
2019-10
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Luminal A breast cancer is a kind of breast cancer with low rate lymph node metastasis and good survival But in clinical practice Luminal A breast cancer can present with early unexpected lymph node metastasis some time indicates poor survival Silent information regulator 2 homolog 1 SIRT1 plays a different role in breast cancer with different molecular typing Previous study supports a role of SIRT1 protein as tumor suppressor in Luminal A breast cancer in association with apoptosis-related proteins The epithelial-to-mesenchymal transitionEMT process results in loss of cell-cell adhesion increased cell mobility and is crucial for enabling the metastasis of cancer cells But no similar study in Luminal A breast cancer Hence this study will 1 investigate the expression pattern of SIRT1 in primary tumor and lymph node metastasis 2 investigate the different expression pattern of SIRT1 in T2T3 lymph node negative tumor and T1 lymph node positive tumor 3 investigate potential role of SIRT1 enzyme in regulating cell migration and invasion in Luminal A breast cancer cells
Detailed Description:
The study has three parts
1 In large specimens of human Luminal A breast cancer with T1 tumor and positive axillary lymph node study the difference expression of SIRT1 and related p53 Bcl-2 autophagy-related protein caspase-3 apaf-1 between primary tumor and lymph node metastases Collect 50 pairs of T1 primary tumors and corresponding metastatic lymph node specimens Using immunohistochemistry anti-SIRT1 p53 Bcl-2 caspase-3 and apaf-1 antibody staining to identify the expression of above proteins in the primary tumor and lymph node metastases
2 Eighty patients with Luminal type A breast cancer T1N were enrolled in this study At the same timeeighty patients were enrolled in the paraffin-embedded specimens of the patients with T2N and T3Ntoo And all patients were followed up Immunohistochemical staining with anti-SIRT1 p53 Bcl-2 caspase-3 apaf-1 E-cadherin N-cadherin Vimentin antibodies to determine the relationship between above protein expression and routine clinicopathological and survival
3 Explore the involvement of SIRT1 in hormone receptor-positive human breast cancer cells at the cellular level The molecular mechanism of EMT-related protein regulation which affects the proliferation invasion and metastasis of tumor cells
1 In large specimens of human Luminal A breast cancer with T1 tumor and positive axillary lymph node study the difference expression of SIRT1 and related p53 Bcl-2 autophagy-related protein caspase-3 apaf-1 between primary tumor and lymph node metastases Collect 50 pairs of T1 primary tumors and corresponding metastatic lymph node specimens Using immunohistochemistry anti-SIRT1 p53 Bcl-2 caspase-3 and apaf-1 antibody staining to identify the expression of above proteins in the primary tumor and lymph node metastases
2 Eighty patients with Luminal type A breast cancer T1N were enrolled in this study At the same timeeighty patients were enrolled in the paraffin-embedded specimens of the patients with T2N and T3Ntoo And all patients were followed up Immunohistochemical staining with anti-SIRT1 p53 Bcl-2 caspase-3 apaf-1 E-cadherin N-cadherin Vimentin antibodies to determine the relationship between above protein expression and routine clinicopathological and survival
3 Explore the involvement of SIRT1 in hormone receptor-positive human breast cancer cells at the cellular level The molecular mechanism of EMT-related protein regulation which affects the proliferation invasion and metastasis of tumor cells
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None