Ignite Creation Date:
2024-05-06 @ 1:46 PM
Last Modification Date:
2024-10-26 @ 1:19 PM
Study NCT ID:
NCT04113928
Status:
COMPLETED
Last Update Posted:
2019-10-03
First Post:
2019-09-17
Brief Title:
Broccoli Ileo Study
Sponsor:
University of Ulster
Organization:
University of Ulster
Study Overview
Official Title:
Maximising the Bioavailability and Activity of Broccoli Phytochemicals Using a Broccoli Soup Enriched for Myrosinase by Addition of Mustard Seeds
Status:
COMPLETED
Status Verified Date:
2019-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Epidemiological and experimental studies have indicated that the consumption of diets rich in cruciferous vegetables such as broccoli has a range of beneficial effects on human health These effects are usually attributed to naturally-occurring glucosinolates and their breakdown products isothiocyanates in cruciferous vegetables One of these compounds sulforaphane the hydrolysis product of glucoraphanin the main glucosinolate in broccoli has been reported to have bactericidal activity against H pylori and other human pathogens The investigators have recently shown that adding mustard seeds which contain a more resilient form of this enzyme to processed broccoli actually increased the formation of sulforaphane and minimised production of another biologically-inactive form Experimental studies by the investigators have shown that sulforaphane can inhibit growth of a number of enteric pathogens including salmonella and E coli which exert their effects in the small intestine Currently the bioavailability stability and bioactivity of sulforaphane in the small intestine of a human following consumption of broccoli is not known
To assess the effect of broccoli phytochemicals in vivo 20 participants who have previously had an ileostomy removal of colon 15 years post operative will be fed 200 ml of broccoli soupcontrol in a randomised double blind crossover design and collect the ileal fluid before 0 hr and after 4 hr the feeding The ileal fluid collected from participants will have undergone in vivo digestion allowing analysis of the chemical composition and bioactivity of the ileal fluid The hypothesis to be tested is that consumption of cooked broccoli plus myrosinase from mustard seeds will result in high levels of sulforaphane in ileostomy fluid sufficient to suppress growth of enteric pathogens This study will be used to assess whether previous observations on the in vitro antibacterial activity of broccoli are relevant in vivo
To assess the effect of broccoli phytochemicals in vivo 20 participants who have previously had an ileostomy removal of colon 15 years post operative will be fed 200 ml of broccoli soupcontrol in a randomised double blind crossover design and collect the ileal fluid before 0 hr and after 4 hr the feeding The ileal fluid collected from participants will have undergone in vivo digestion allowing analysis of the chemical composition and bioactivity of the ileal fluid The hypothesis to be tested is that consumption of cooked broccoli plus myrosinase from mustard seeds will result in high levels of sulforaphane in ileostomy fluid sufficient to suppress growth of enteric pathogens This study will be used to assess whether previous observations on the in vitro antibacterial activity of broccoli are relevant in vivo
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None