Ignite Creation Date:
2024-05-05 @ 4:58 PM
Last Modification Date:
2024-10-26 @ 9:26 AM
Study NCT ID:
NCT00353730
Status:
COMPLETED
Last Update Posted:
2021-09-08
First Post:
2006-07-18
Brief Title:
Identification of Genes Expressed in Atherosclerotic Plaque
Sponsor:
National Heart Lung and Blood Institute NHLBI
Organization:
National Institutes of Health Clinical Center CC
Study Overview
Official Title:
Identification of Genes Expressed in Atherosclerotic Plaques
Status:
COMPLETED
Status Verified Date:
2021-09
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
This study will identify genes and proteins in the blood of patients with atherosclerosis and in that of normal volunteers The findings will be compared to determine the influence of these substances on the development of atherosclerosis a narrowing and hardening of blood vessel walls by deposits of fatty substances Blood vessel blockage caused by atherosclerosis can impede blood flow and cause stroke heart attack and poor limb circulation The information from this study may lead to better ways to detect prevent and treat these diseases
Healthy volunteers and patients scheduled for carotid endarterectomy at Suburban Hospital in Bethesda Maryland are eligible for this study Carotid endarterectomy is a surgical procedure in which the inner layer of the carotid artery neck artery supplying blood to the brain is scraped away to open the blocked vessel Participants will undergo the following tests and procedures
Patients
Blood sample Collection of about 32 cc 8 tablespoons of blood for genetic and protein analysis
Tissue sample Collection of a piece of diseased blood vessel discarded from the endarterectomy procedure
Review of records Review of medical records for information about past illnesses medications tests and so forth if needed
Normal Volunteers
Blood samples Collection of about 32 cc 8 tablespoons of blood for genetic and protein analysis
Carotid artery ultrasound Ultrasound imaging of the neck arteries for detection of any blockage
Electrocardiogram Recording of the electrical activity of the heart to detect any abnormalities in heart rhythm
Echocardiogram Ultrasound examination to detect possible abnormalities of the heart muscle
Cardiac stress test Treadmill stress test to detect possible heart vessel blockage for subjects who have not had a cardiac stress test in the past year
Review of records Review of medical records for information about past illnesses medications tests and so forth if needed
Healthy volunteers and patients scheduled for carotid endarterectomy at Suburban Hospital in Bethesda Maryland are eligible for this study Carotid endarterectomy is a surgical procedure in which the inner layer of the carotid artery neck artery supplying blood to the brain is scraped away to open the blocked vessel Participants will undergo the following tests and procedures
Patients
Blood sample Collection of about 32 cc 8 tablespoons of blood for genetic and protein analysis
Tissue sample Collection of a piece of diseased blood vessel discarded from the endarterectomy procedure
Review of records Review of medical records for information about past illnesses medications tests and so forth if needed
Normal Volunteers
Blood samples Collection of about 32 cc 8 tablespoons of blood for genetic and protein analysis
Carotid artery ultrasound Ultrasound imaging of the neck arteries for detection of any blockage
Electrocardiogram Recording of the electrical activity of the heart to detect any abnormalities in heart rhythm
Echocardiogram Ultrasound examination to detect possible abnormalities of the heart muscle
Cardiac stress test Treadmill stress test to detect possible heart vessel blockage for subjects who have not had a cardiac stress test in the past year
Review of records Review of medical records for information about past illnesses medications tests and so forth if needed
Detailed Description:
Significant progress has been made in the prevention and treatment of atherosclerosis but it continues to be a leading cause of morbidity and mortality in our society The problem will become even more significant as an increasing proportion of the population ages The goal of the proposed project is to advance our understanding of the genetic mechanisms of atherosclerosis We propose to identify genes selectively expressed in peripheral monocytes of patients with atherosclerosis and in macrophages and endothelial cells of atherosclerotic plaques We will examine gene expression profiles in purified subpopulations of cells using Serial Analysis of Gene Expression SAGE that requires only minute amounts of RNA enabling the study of small homogeneous populations of cells This sequence-based high-throughput gene expression profiling technique has the advantage of not requiring prior knowledge of the expressed gene sequences allowing the discovery of new genes With the completion of the human genome sequence the SAGE technique is an ideal tool for comprehensively characterizing the transcriptomes of the important cells involved in atherosclerosis This proposal is an exploratory study to find candidates both known and unknown genes which will be used for more extensive and statistically powered projects in the future The insights obtained will be used for elucidating the pathogenesis of atherosclerosis and for developing new simple sensitive and specific tests for early disease diagnosis In addition the new insights obtained may lead to the development of novel therapeutic strategies for disease prevention and treatment
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 02-H-0265 | None | None | None |