Ignite Creation Date:
2024-05-06 @ 1:06 PM
Last Modification Date:
2024-10-26 @ 1:09 PM
Study NCT ID:
NCT03934905
Status:
RECRUITING
Last Update Posted:
2023-08-07
First Post:
2019-03-29
Brief Title:
Protective Effects of the Nutritional Supplement Sulforaphane on Doxorubicin-Associated Cardiac Dysfunction
Sponsor:
Texas Tech University Health Sciences Center
Organization:
Texas Tech University Health Sciences Center
Study Overview
Official Title:
Phase II Trial of Effects of the Nutritional Supplement Sulforaphane on Doxorubicin-Associated Cardiac Dysfunction CRI18-026
Status:
RECRUITING
Status Verified Date:
2023-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
No
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Cardiomyopathy is a major complication of doxorubicin DOX chemotherapy and 10-21 of breast cancer patients receiving DOX experience compromised cardiac function Recent advancements have increased cancer survivorship but it remains clinically challenging to mitigate the cardiotoxic side effects Although there are several strategies used to reduce the occurrence and severity of DOX-induced cardiotoxicity they are not particularly effective Hence there is an urgent need to develop new strategies that prevent the cardiotoxic effects of DOX but maintain its potency as a cancer therapy Because the cellular events responsible for the antitumor activity of DOX and DOX-induced cardiotoxicity are distinctly different it may be possible to develop therapies that selectively mitigate DOX-induced cardiotoxicity Thus the investigators propose to test an adjuvant therapy that combines the phytochemical sulforaphane SFN with DOX to attenuate DOX-induced cardiomyopathy SFN activates the transcription factor Nrf2 and induces defense mechanisms in normal cells Furthermore SFN inhibits carcinogenesis and metastases and enhances cancer cell sensitivity to DOX seemingly through Nrf2-independent mechanisms SFN has also been tested in several clinical trials although never together with DOX Our early animal studies suggest that by activating Nrf2 SFN selectively protects the mouse and rat from DOX cardiotoxicity enhances survival and enhances the effects of DOX on cancer growth in a rat breast cancer model The investigators suspect that SFN affects DOX metabolism in cancer cells to enhance tumor regression or it may synergistically activate other key antitumor mechanisms Hence SFN may improve the clinical outcome of cancer therapy by 1 attenuating DOX cardiotoxicity and 2 enhancing the effects of cancer treatment on the tumor Our hypothesis is that SFN protects the heart from DOX-mediated cardiac injury without altering the antitumor efficacy of DOX In Aim 1 the investigators will conduct an early-phase clinical trial to determine if SFN is safe to administer to breast cancer patients undergoing DOX chemotherapy In Aim 2 the investigators will determine if SFN decreases DOX-induced inflammatory responses and enhances Nrf2- and SIRT1-target gene expression in breast cancer patients Notably transcript and protein signatures in peripheral blood mononuclear cells PBMCs can predict cardiac function in patients undergoing DOX chemotherapy for breast cancer The investigators will also determine if SFNDOX treatment activates Nrf2- and SIRT1-dependent gene expression alters the levels of biomarkers for presymptomatic DOX-cardiotoxicity and mitigates the generation of cardiotoxic metabolites in PBMCs and plasma These studies will facilitate the development of SFN co-treatment as a strategy to enhance the efficacy and safety of DOX cancer therapy
Detailed Description:
Determine whether nutritional supplement sulforaphane SFN is safe to administer to breast cancer patients undergoing doxorubicin DOX chemotherapy The investigators have identified biomarkers for presymptomatic detection of DOX cardiotoxicity in breast cancer patients and reported that SFN alleviates DOX-induced cardiac toxicity while maintaining its anti-tumor activity in an animal model of breast cancer SFN is in various stages of preclinical and clinical trials against different types of cancer but its safety and effects on identified markers have never been tested in patients treated with DOX Sulforaphane is a generally recognized as a safe GRAS compound and this compound is currently in 61 different clinical trials including Cystic Fibrosis COPD Melanoma Breast and prostate cancer etc httpsclinicaltrialsgovct2resultstermsulforaphanepg1 Avmacol Extra Strength Nutramax Laboratories Consumer Care Inc Edgewood MD 21040 an over-the-counter dietary supplement containing broccoli seed and sprout extracts that is rich with sulforaphane will be used in this study This is an FDA regulated trial using an investigational drug under IND 141682 Dose considerations were made on the basis of the ongoing clinical trial Effects of Avmacol Extra Strength in the Oral Mucosa of Patients Following Curative Treatment for Tobacco-related Head and Neck Cancer httpsclinicaltrialsgovct2showNCT03268993 Furthermore Avmacol Extra Strength has never been tested as an adjuvant to try and protect the heart from DOXs harmful effects Therefore the investigators are proposing to do an early-clinical trial to assess SFN safety in DOX-treated patients and possibly prevent DOX-cardiotoxicity in breast cancer patients
Our hypothesis is that SFN protects the heart from DOX-mediated cardiac injury without affecting its antitumor efficacy Here the investigators will test SFN in combination with DOX-based chemotherapy for breast cancer the investigators will assess its safety cardio-protective properties and anti-cancer efficacy The investigators will also examine the effect of SFN on the expression of several biomarkers that indicate when a patient cannot tolerate DOX1 To test our hypothesis the investigators propose the following Specific Aims
Aim 1 Demonstrate that co-administration of SFN protects the heart and is not associated with toxicity or reduced tumor response in breast cancer patients undergoing DOX chemotherapy
The investigators propose a pilot clinical trial to assess the safety of SFN as a co-treatment with DOX chemotherapy Strategy Up to seventy breast cancer patients prescribed DOX chemotherapy will be consented then randomly assigned to receive either SFN or placebo during DOX chemotherapy This sample size includes an additional 10 participants to allow for potential attrition There will be a 50-50 chance of receiving SFN The patients will be recruited from the Breast Cancer Clinic Dr Jones and Philipovskiy in the Southwest Cancer Center at TTUHSCUMC Lubbock Cardiac function with echocardiography will be assessed and compared between arms for evidence of substantive decrease in DOX cardiotoxicity with SFN compared to placebo Tumor size will also be compared based on RECIST criteria between treatment arms The investigators will monitor treatment-emergent symptoms hematological parameters of cancer therapy toxicity and renal and hepatic function Blood samples will be collected to assay markers of cardiotoxicity including B-type natriuretic peptide and troponin
Aim 2 Determine if SFN alters the levels of known biomarkers of DOX cardiotoxicity and affects the expression of SIRT1- and Nrf2-target genes
The investigators will measure changes in transcript and protein biomarkers of pre-symptomatic DOX-cardiotoxicity in response to SFN and will determine if SFN promotes SIRT1- and Nrf2-dependent gene expression Because Nrf2 upregulates certain detoxifying enzymes the investigators will measure the plasma levels of cardiotoxic metabolites doxorubicinol DOXol Strategy Blood will be collected before the start of DOX chemotherapy and after each treatment cycle to isolate peripheral blood mononuclear cells PBMCs and prepare plasma In lieu of tissue biopsies PBMCs will be used to examine SIRT1 and Nrf2 activity and target gene expression Multiplex arrays will be used to measure plasma cytokine levels and plasma DOX metabolites will be assessed with ultrahigh performance liquid chromatography-tandem mass spectrometry These experiments will provide initial evidence to indicate that SFN activates SIRT1 and Nrf2 pathways in non-cancer tissue of breast cancer patients
Impact This will be the first clinical study to assess the safety of SFN as a co-treatment with DOX to mitigate cardiotoxicity in breast cancer patients Positive findings will be used to justify a larger randomized controlled trial Subjects will be randomized at a 11 ratio
Study Design and Procedures Study Design The investigators intend to include up to 70 DOX-naïve women diagnosed with breast cancer undergoing neoadjuvant chemotherapy with no prior cardiac disease and who will receive DOX without Her-2 receptor antagonists to eliminate possibility of secondary side effects as part of their clinical care These potential subjects will be recruited in a randomized controlled double blinded pilot study comparing SFN to placebo The study will be conducted at the Texas Tech University Health Sciences Center and University Medical Center The trial will be approved by our local IRB and registered at clinicaltrialsgov UMC cancer center pharmacist Ajoke A Tijani RPh Tijaniumchealthsystemcom will receive placebo and test compound from supplier and will dispense them to test subjects
Randomization will be achieved with assignment of a subject ID to the study subject The list of subject IDs will be subsequently assigned either study drug or placebo using a randomizer software such as wwwrandomizerorg The pharmacist dispensing the medication will be aware of the study IDs in relation to the patient identity in order to be able to dispense the test drug or the placebo per their assigned status as test or control subjects
Study Drug and Placebo Processed SFN-rich extract will be purchased in form of caplets from Nutramax Laboratories Inc 2208 Lakeside Blvd Edgewood MD 21040 Caplets containing SFN-rich broccoli sprout extracts or microcrystalline cellulose placebo also from Nutramax Labs will be dispensed to participants in sealed bottles with instructions to keep them in a household freezer Size of the caplet will be about the size of a 1000 mg Vit C pill about 2 cm in length The participants will be dosed based on weight in a double-blind fashion with identical appearing placebo or SFN caplets in a daily dose for 12 weeks of two caplets for individuals 100 lb three caplets for individuals 100-200 lb and four caplets for individuals 200 lb Avmacol Extra Strength or placebo will be prescribed by Dr Jones and Philipovskiy and will be dispensed by local pharmacy or study coordinators at TTUHSCUMC Lubbock The investigators will be doing pill counts to make sure that volunteers have used as directed The investigators will measure the Sulforaphane level in plasma by well-established method 26 The drug and the placebo will be stored at the South West Cancer Center pharmacy based on manufacturer based guidelines and dispensed to each participant at their baseline visit then at DOX infusion visits 1 2 and 3 Patients will be expected to maintain at least an 80 adherence to the medication regimen in the absence of prohibitive toxicity Adherence will be monitored through therapeutic drug level Plasma sulforaphane levels of 120 ngml monitoring as mentioned above and pill counts For subjects who do not meet 80 compliance they will be instructed on the importance of taking the pills as directed but if their next visit demonstrates less than 80 compliance they will be withdrawn from the study As long as participants complete their 4th cycle of DOX chemotherapy treatment the investigators will still use their study data during analysis If side effects are noted patient will be asked to notify the study team of the same and will be evaluated within a suitable time frame based on severity of side effects
SFN is a safe natural isolate Above doses 30mgcaplet are considered adequate to maintain intended therapeutic drug levels while maintaining a simple study design and without significant concerns for drug toxicity It has been used in several clinical trials from doses ranging from 2-200 µmolday for 2-28 weeks in 25-80 years old subjects without significant side-effects or toxicity httpsclinicaltrialsgovct2resultstermsulforaphaneSearchSearch yet never in combination with DOX
Statistical Considerations Data for the primary endpoint DOX cardiotoxicity and other binary outcomes will be summarized by treatment arm as number and proportion per arm The change in DOX-cardiotoxicity rate with SFN compared to placebo will be assessed with a 1-sided Z-test see Sample-size justification Tumor size plasma cardiac biomarkers cardiac ejection fraction DOX metabolite levels will be summarized by treatment arm and time point as means and standard deviations and graphed as box plots and profile plots The investigators will adhere to ANOVA distributional assumptions so that appropriate data transformations can be applied Data for each continuous variable will be analyzed for differences in group means at each time point with ANOVA or Mann-Whitney-Wilcoxon non-parametric tests at each time point These tests will employ a more-stringent alpha 002 significance level to adjust for the multiple comparisons without overinflating type II error For subjects withdrawn from the study or do not complete the study for any reason as long as they completed their 4th cycle of DOX chemotherapy treatment the investigators will still use their study data during analysis
Sample-size justification The number of subjects 35 per treatment arm 70 total the investigators expect some attrition which this sample size addresses is based on the primary endpoint of DOX-induced cardiac dysfunction the rate of which was recently observed at our previous institution UAMS to be 251 The investigators expect that SFN will decrease this to 5 The cardiotoxicity rates for each arm will be compared via 1-sided pooled-variance Z-test at an alpha 010 The statistical power of this test conducted as described needs to exceed 80 in order for our study to be generally recognized as having adequate statistical power Our sample size provides the 1-sided pooled-variance Z-test with 832 power at 10 alpha to detect the expected 20-point decrease in DOX-toxicity rates from 25 in the placebo arm to 5 in the SFN arm
The formal consent of each subject using the IRB-approved consent form will be obtained before that subject is submitted to any study procedure
Our hypothesis is that SFN protects the heart from DOX-mediated cardiac injury without affecting its antitumor efficacy Here the investigators will test SFN in combination with DOX-based chemotherapy for breast cancer the investigators will assess its safety cardio-protective properties and anti-cancer efficacy The investigators will also examine the effect of SFN on the expression of several biomarkers that indicate when a patient cannot tolerate DOX1 To test our hypothesis the investigators propose the following Specific Aims
Aim 1 Demonstrate that co-administration of SFN protects the heart and is not associated with toxicity or reduced tumor response in breast cancer patients undergoing DOX chemotherapy
The investigators propose a pilot clinical trial to assess the safety of SFN as a co-treatment with DOX chemotherapy Strategy Up to seventy breast cancer patients prescribed DOX chemotherapy will be consented then randomly assigned to receive either SFN or placebo during DOX chemotherapy This sample size includes an additional 10 participants to allow for potential attrition There will be a 50-50 chance of receiving SFN The patients will be recruited from the Breast Cancer Clinic Dr Jones and Philipovskiy in the Southwest Cancer Center at TTUHSCUMC Lubbock Cardiac function with echocardiography will be assessed and compared between arms for evidence of substantive decrease in DOX cardiotoxicity with SFN compared to placebo Tumor size will also be compared based on RECIST criteria between treatment arms The investigators will monitor treatment-emergent symptoms hematological parameters of cancer therapy toxicity and renal and hepatic function Blood samples will be collected to assay markers of cardiotoxicity including B-type natriuretic peptide and troponin
Aim 2 Determine if SFN alters the levels of known biomarkers of DOX cardiotoxicity and affects the expression of SIRT1- and Nrf2-target genes
The investigators will measure changes in transcript and protein biomarkers of pre-symptomatic DOX-cardiotoxicity in response to SFN and will determine if SFN promotes SIRT1- and Nrf2-dependent gene expression Because Nrf2 upregulates certain detoxifying enzymes the investigators will measure the plasma levels of cardiotoxic metabolites doxorubicinol DOXol Strategy Blood will be collected before the start of DOX chemotherapy and after each treatment cycle to isolate peripheral blood mononuclear cells PBMCs and prepare plasma In lieu of tissue biopsies PBMCs will be used to examine SIRT1 and Nrf2 activity and target gene expression Multiplex arrays will be used to measure plasma cytokine levels and plasma DOX metabolites will be assessed with ultrahigh performance liquid chromatography-tandem mass spectrometry These experiments will provide initial evidence to indicate that SFN activates SIRT1 and Nrf2 pathways in non-cancer tissue of breast cancer patients
Impact This will be the first clinical study to assess the safety of SFN as a co-treatment with DOX to mitigate cardiotoxicity in breast cancer patients Positive findings will be used to justify a larger randomized controlled trial Subjects will be randomized at a 11 ratio
Study Design and Procedures Study Design The investigators intend to include up to 70 DOX-naïve women diagnosed with breast cancer undergoing neoadjuvant chemotherapy with no prior cardiac disease and who will receive DOX without Her-2 receptor antagonists to eliminate possibility of secondary side effects as part of their clinical care These potential subjects will be recruited in a randomized controlled double blinded pilot study comparing SFN to placebo The study will be conducted at the Texas Tech University Health Sciences Center and University Medical Center The trial will be approved by our local IRB and registered at clinicaltrialsgov UMC cancer center pharmacist Ajoke A Tijani RPh Tijaniumchealthsystemcom will receive placebo and test compound from supplier and will dispense them to test subjects
Randomization will be achieved with assignment of a subject ID to the study subject The list of subject IDs will be subsequently assigned either study drug or placebo using a randomizer software such as wwwrandomizerorg The pharmacist dispensing the medication will be aware of the study IDs in relation to the patient identity in order to be able to dispense the test drug or the placebo per their assigned status as test or control subjects
Study Drug and Placebo Processed SFN-rich extract will be purchased in form of caplets from Nutramax Laboratories Inc 2208 Lakeside Blvd Edgewood MD 21040 Caplets containing SFN-rich broccoli sprout extracts or microcrystalline cellulose placebo also from Nutramax Labs will be dispensed to participants in sealed bottles with instructions to keep them in a household freezer Size of the caplet will be about the size of a 1000 mg Vit C pill about 2 cm in length The participants will be dosed based on weight in a double-blind fashion with identical appearing placebo or SFN caplets in a daily dose for 12 weeks of two caplets for individuals 100 lb three caplets for individuals 100-200 lb and four caplets for individuals 200 lb Avmacol Extra Strength or placebo will be prescribed by Dr Jones and Philipovskiy and will be dispensed by local pharmacy or study coordinators at TTUHSCUMC Lubbock The investigators will be doing pill counts to make sure that volunteers have used as directed The investigators will measure the Sulforaphane level in plasma by well-established method 26 The drug and the placebo will be stored at the South West Cancer Center pharmacy based on manufacturer based guidelines and dispensed to each participant at their baseline visit then at DOX infusion visits 1 2 and 3 Patients will be expected to maintain at least an 80 adherence to the medication regimen in the absence of prohibitive toxicity Adherence will be monitored through therapeutic drug level Plasma sulforaphane levels of 120 ngml monitoring as mentioned above and pill counts For subjects who do not meet 80 compliance they will be instructed on the importance of taking the pills as directed but if their next visit demonstrates less than 80 compliance they will be withdrawn from the study As long as participants complete their 4th cycle of DOX chemotherapy treatment the investigators will still use their study data during analysis If side effects are noted patient will be asked to notify the study team of the same and will be evaluated within a suitable time frame based on severity of side effects
SFN is a safe natural isolate Above doses 30mgcaplet are considered adequate to maintain intended therapeutic drug levels while maintaining a simple study design and without significant concerns for drug toxicity It has been used in several clinical trials from doses ranging from 2-200 µmolday for 2-28 weeks in 25-80 years old subjects without significant side-effects or toxicity httpsclinicaltrialsgovct2resultstermsulforaphaneSearchSearch yet never in combination with DOX
Statistical Considerations Data for the primary endpoint DOX cardiotoxicity and other binary outcomes will be summarized by treatment arm as number and proportion per arm The change in DOX-cardiotoxicity rate with SFN compared to placebo will be assessed with a 1-sided Z-test see Sample-size justification Tumor size plasma cardiac biomarkers cardiac ejection fraction DOX metabolite levels will be summarized by treatment arm and time point as means and standard deviations and graphed as box plots and profile plots The investigators will adhere to ANOVA distributional assumptions so that appropriate data transformations can be applied Data for each continuous variable will be analyzed for differences in group means at each time point with ANOVA or Mann-Whitney-Wilcoxon non-parametric tests at each time point These tests will employ a more-stringent alpha 002 significance level to adjust for the multiple comparisons without overinflating type II error For subjects withdrawn from the study or do not complete the study for any reason as long as they completed their 4th cycle of DOX chemotherapy treatment the investigators will still use their study data during analysis
Sample-size justification The number of subjects 35 per treatment arm 70 total the investigators expect some attrition which this sample size addresses is based on the primary endpoint of DOX-induced cardiac dysfunction the rate of which was recently observed at our previous institution UAMS to be 251 The investigators expect that SFN will decrease this to 5 The cardiotoxicity rates for each arm will be compared via 1-sided pooled-variance Z-test at an alpha 010 The statistical power of this test conducted as described needs to exceed 80 in order for our study to be generally recognized as having adequate statistical power Our sample size provides the 1-sided pooled-variance Z-test with 832 power at 10 alpha to detect the expected 20-point decrease in DOX-toxicity rates from 25 in the placebo arm to 5 in the SFN arm
The formal consent of each subject using the IRB-approved consent form will be obtained before that subject is submitted to any study procedure
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
True
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None