Viewing Study NCT03927872



Ignite Creation Date: 2024-05-06 @ 1:04 PM
Last Modification Date: 2024-10-26 @ 1:08 PM
Study NCT ID: NCT03927872
Status: COMPLETED
Last Update Posted: 2019-04-30
First Post: 2019-04-23

Brief Title: Cardio-embolic Stroke and Blood Bio-markers
Sponsor: Wafaa Samir
Organization: Zagazig University

Study Overview

Official Title: Cardio-embolic Stroke and Blood Bio-markersDiagnosis and Predictors of Short Term Outcome
Status: COMPLETED
Status Verified Date: 2019-04
Last Known Status: None
Delayed Posting: No
If Stopped, Why?: Not Stopped
Has Expanded Access: False
If Expanded Access, NCT#: N/A
Has Expanded Access, NCT# Status: N/A
Acronym: None
Brief Summary: Brain is a productive source of variety of enzymes and any brain injury like stroke to brain tissue could similarly result in an increase in these enzymes in cerebrospinal fluid and serum Evaluation of these enzymes represent a simple method for the ischemic stroke subtype diagnosis and prognosis Objective The aim of this study was to determine the role Brain natriuretic peptide BNP D-Dimer creatine kinase- MBCK-MB C reactive protein CRP serum levels and GA ratio in diagnosis of CES stroke and its ability to predict short term outcome Methods This study was conducted on 96 patients with acute ischemic stroke subdivided into two groups group Ι was 48 patients with cardio-embolic stroke and group ΙΙ was 48 patients with non- cardio-embolic all patients were subjected to assessment of serum BNP D-Dimer and CK-MB and CRP and globulin albumin ratio within the first 24 h of stroke At third week they were assessed by mRS
Detailed Description: participants and methods 96 acute ischemic stroke patients divided to two groups group Ι 48 patients with cardio-embolic stroke CES and group ΙΙ 48 patients with cerebrovascular stroke other than cardio-embolic stroke non CES with first-ever acute ischemic stroke within 24 h of onset of symptoms admitted to Intensive Care and Stroke Unit

All patients were subjected to full detailed neurological history with stressing on the vascular and cardiac risk factors and general and neurological examination Stroke severity was assessed using Glasgow Coma Scale GCS and the National Institutes of Health Stroke Scale NIHSS

Laboratory investigations15 ml venous blood were obtained from the patients within the first 24 hours of stroke All routine and special laboratory investigations were done at Clinical pathology department Zagazig university hospitals including CBC liver function kidney function ESR and lipid profile

Brain natriuretic peptide 4 ml blood was placed in a polypro pane tube containing a mixture of sodium salt of ethylene diamine tetra acetic acid EDTA at a concentration of 1mg ml of blood and aprotinin at a concentration of 500 kallikerin inhibitory unit kiu ml of blood serving as a natural proteinase inhibitor which act as a preservative for natriuretic peptides then mixing of the blood was done then the tube was centrifuged at 5000 rpm for 15 minutes in the cool centrifuge the separated serum was stored at -70 degree c until analysis

Later on the assay was done using human BNP Elisa kits Spain react kits sr 19369u which are immune-sorbent assay for quantitative measurement the least sensitivity is 14 pgml and ranges 14 -1000pgml

D-Dimer 3ml blood was collected within a vacuum tubes containing 05 ml buffered sodium citrate before the initiation of any oral enteral or parenteral feeding or medications Blood samples were mixed well with avoiding of foam formation then immediately centrifuged at 3000 rpm for 15 min at ambient room temperature and plasma is separated D-Dimer was measured turbidmetry SAT Liatest D-Di Diagnostica STAGO on the STAgo compact analyzer Normal value is less than 500 ng ml 15

Creatine Kinase MB 4 ml blood were derived within a plastic tube centrifuged at 4000rpm for 3minutes the separated serum was assayed for CK MB using Spain react kits with normal value up to 28ng L C reactive protein 4ml were withdrawn in a plastic tube centrifuged at 4000rpm for 3minutes the separated serum used for analysis of CRP measured using Olympus 640 analyzer with normal value up to 5 mg L GlobulinAlbumin ratio GA was calculated with normal ratio 06-07 All patients were evaluated by Brain CT or MRI for diagnosis of ischemic stroke

Each patient underwent to Electrocardiogram Echocardiography and Carotid Doppler ultrasonography to define etiological stoke subtype

Study Oversight

Has Oversight DMC: None
Is a FDA Regulated Drug?: False
Is a FDA Regulated Device?: False
Is an Unapproved Device?: None
Is a PPSD?: None
Is a US Export?: None
Is an FDA AA801 Violation?: None