Ignite Creation Date:
2024-05-05 @ 11:08 AM
Last Modification Date:
2024-10-26 @ 9:02 AM
Study NCT ID:
NCT00001726
Status:
COMPLETED
Last Update Posted:
2017-07-02
First Post:
1999-11-03
Brief Title:
Study of the Composition of Dental Plaque
Sponsor:
National Institute of Dental and Craniofacial Research NIDCR
Organization:
National Institutes of Health Clinical Center CC
Study Overview
Official Title:
Spatial Organization of Viridans Streptococci in Oral Biofilms
Status:
COMPLETED
Status Verified Date:
2009-08-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
This study will examine the composition of dental plaque-a naturally occurring substance that sticks to the teeth and can cause tooth decay and gum disease A better understanding of how plaque builds up in the mouth may help in developing improved ways of controlling it
Healthy normal volunteers between the ages of 18 and 65 who work at the National Institutes of Health main campus in Bethesda Maryland may participate in this study Candidates will be screened for eligibility with a medical and dental history
This study involves a maximum of five visits to the dental clinic At the first visit participants will have a dental examination and a mold will be made of the mouth To make the mold a small plastic tray containing impression material will be placed in the volunteers mouth and held in place for about 2 minutes to set The tray will then be removed and a mold will be made from the impression The mold will be used to make the mouthpiece used in study 1 described below and the tooth fittings used in study 2 also described below Volunteers will participate in one of these two studies
Study 1 Volunteers in study 1 will have their mouthpiece checked at the second visit have a teeth cleaning and have the mold put in place The mouthpiece will be worn for up to 8 hours during which time soft foods can be eaten Mouthwash should not be used while the device is in place At the volunteers third last visit the mouthpiece will be removed and the volunteer will spit into a tube to collect saliva for examination for bacteria
Study 2 Volunteers in study 2 will have their teeth cleaned at the second visit and the tooth fittings placed onto the back teeth with a dental adhesive The fittings will be worn for up to 72 hours during which time volunteers can eat a regular diet and brush their teeth Mouthwash should not be used while the fittings are in place At the third fourth and fifth visits some of the fittings will be removed and if necessary the tooth surface will be polished The last of the fittings will be removed at the fifth visit and the volunteer will spit into a tube to collect saliva for examination for bacteria
Healthy normal volunteers between the ages of 18 and 65 who work at the National Institutes of Health main campus in Bethesda Maryland may participate in this study Candidates will be screened for eligibility with a medical and dental history
This study involves a maximum of five visits to the dental clinic At the first visit participants will have a dental examination and a mold will be made of the mouth To make the mold a small plastic tray containing impression material will be placed in the volunteers mouth and held in place for about 2 minutes to set The tray will then be removed and a mold will be made from the impression The mold will be used to make the mouthpiece used in study 1 described below and the tooth fittings used in study 2 also described below Volunteers will participate in one of these two studies
Study 1 Volunteers in study 1 will have their mouthpiece checked at the second visit have a teeth cleaning and have the mold put in place The mouthpiece will be worn for up to 8 hours during which time soft foods can be eaten Mouthwash should not be used while the device is in place At the volunteers third last visit the mouthpiece will be removed and the volunteer will spit into a tube to collect saliva for examination for bacteria
Study 2 Volunteers in study 2 will have their teeth cleaned at the second visit and the tooth fittings placed onto the back teeth with a dental adhesive The fittings will be worn for up to 72 hours during which time volunteers can eat a regular diet and brush their teeth Mouthwash should not be used while the fittings are in place At the third fourth and fifth visits some of the fittings will be removed and if necessary the tooth surface will be polished The last of the fittings will be removed at the fifth visit and the volunteer will spit into a tube to collect saliva for examination for bacteria
Detailed Description:
Dental caries and periodontal disease are closely associated with dental plaque the biofilm that results from microbial colonization of the tooth surface Various experimental models have been described for the initial phase of colonization including one that utilizes enamel chips positioned within the oral cavity for periods of time up to 24 hours Scanning electron microscopy of chips removed after 4 hrs in vivo revealed individual bacteria attached to the pellicle-coated surface Growth of these pioneer organisms resulted in microcolonies that merged to form a spreading monolayer of cocci and rods Over 80 of the bacteria present in early biofilms were identified as members of four closely related viridans streptococcal species Streptococcus sanguis S oralis S mitis and S gordonii Veillonella spp and Actinomyces naeslundii each composed about 5 Interestingly veillonellae actinomyces and streptococci all increased during the first 24 hrs of colonization thereby suggesting important roles for these bacteria in normal daily plaque accumulation early biofilm development
One of the major uncertainties raised by the wide-spread ability of cells of oral bacteria to interact physically in vitro is whether these coaggregations function in vivo Using direct immunofluorescence we have demonstrated the coincident juxtaposition in dental plaque of streptococci and actinomyces suggesting that these known coaggregating partners may use coaggregation as a means to colonize enamel Our overall goal is to determine spatial organization of different viridans streptococci in biofilms that form during initial colonization of the tooth surface These bacteria include two groups with distinctive properties One group contains strains of S sanguis and S gordonii with GalNAc-sensitive adhesions and the other contains strains of S oralis and other species with complementary receptors for intrageneric coaggregation The models for biofilm formation will include an in vitro flow-cell model in which streptococci with known adhesive properties attach and grow on a saliva-coated surface and an in vivo model in which enamel chips are colonized within the oral cavity for periods of time up to 72 hours The results of these studies should provide significant insights into the involvement of interbacterial adhesion in biofilm development Our goal from these studies is to investigate the spatial organization of streptococci and their coaggregation partner actinomyces and veillonellae in biofilms formed in vivo compared to in vitro We hope to make significant advances in understanding the distribution of these species in initial dental plaque formation Since the streptococci are the predominant organism in early dental plaque we propose that understanding their contribution to microbial communities will lead to important advances in designing effective oral health regimens
One of the major uncertainties raised by the wide-spread ability of cells of oral bacteria to interact physically in vitro is whether these coaggregations function in vivo Using direct immunofluorescence we have demonstrated the coincident juxtaposition in dental plaque of streptococci and actinomyces suggesting that these known coaggregating partners may use coaggregation as a means to colonize enamel Our overall goal is to determine spatial organization of different viridans streptococci in biofilms that form during initial colonization of the tooth surface These bacteria include two groups with distinctive properties One group contains strains of S sanguis and S gordonii with GalNAc-sensitive adhesions and the other contains strains of S oralis and other species with complementary receptors for intrageneric coaggregation The models for biofilm formation will include an in vitro flow-cell model in which streptococci with known adhesive properties attach and grow on a saliva-coated surface and an in vivo model in which enamel chips are colonized within the oral cavity for periods of time up to 72 hours The results of these studies should provide significant insights into the involvement of interbacterial adhesion in biofilm development Our goal from these studies is to investigate the spatial organization of streptococci and their coaggregation partner actinomyces and veillonellae in biofilms formed in vivo compared to in vitro We hope to make significant advances in understanding the distribution of these species in initial dental plaque formation Since the streptococci are the predominant organism in early dental plaque we propose that understanding their contribution to microbial communities will lead to important advances in designing effective oral health regimens
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 98-D-0116 | None | None | None |