Ignite Creation Date:
2024-05-05 @ 4:54 PM
Last Modification Date:
2024-10-26 @ 9:25 AM
Study NCT ID:
NCT00340847
Status:
COMPLETED
Last Update Posted:
2017-07-02
First Post:
2006-06-19
Brief Title:
Construction and Use of Lung Tumor Microarray for the Analysis of Gene Expression in Lung Cancer
Sponsor:
National Cancer Institute NCI
Organization:
National Institutes of Health Clinical Center CC
Study Overview
Official Title:
Construction and Use of Lung Tumor Microarray for the Analysis of Gene Expression in Lung Cancer
Status:
COMPLETED
Status Verified Date:
2010-05-19
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The proposed research intends to construct a set of tissue microarrays containing different types of normal and lung cancer tissues for the study of genes associated with lung cancer Thus far we have generated a lung cancer tissue arrayusing paraffin embedded archival tissues from 300 lung turmors tissues and 100 adjacent normal tissues Four- micrometer thickness sections have been cut from the tissue array and were used to survey gene expression status in arrayed tumors using immunohistochemistry methody We are currently performing IHC studies ot 1 determine protein expression and its correlation with gene expression patterms ovserved using cDNA arrays 2 Analyze protein expression in the chromosome remodeling pathyway in non-small cell lung cancer And 3 determine the association of gene expression with lung tumor stage and clinical outcome The current protocol is needed to complete the above studies and for the production of neuroendocrine tumors
Detailed Description:
The proposed research intends to construct a set of tissue microarrays containing different types of lung cancer tissues for the study of genes associated with lung cancer In this study lung tissue arrays will be generated using paraffin embedded archival tissues containing adjacent normal tissues and tumors from approximately 300 non-small cell lung cancers and 100 neuroendocrine tumors of the lung Corded tissues of 06 um in diameter will be taken from each tumor and arrayed onto recipient paraffin blocks to generate a lung tumor specific microarray Four-micrometer thickness sections will be cut from the tissue array and used to survey gene expression status in arrayed tumors using fluorescence in situ hybridization FISH in situ hybridization andor immunohistochemistry methods Additionally approximately 20 sections will be cut from each primary tumor block to isolate genomic DNA for the analysis of genetic changes in the arrayed tumor samples The goals of this proposal are 1 to generate a single tissue block containing multiple tumors 2 allow rapid and efficient analysis of gene expressions in a large number of samples using a single tissue section and 3 to correlate the molecular changes in the tumors with the clinical and pathological features of the disease Due to the experimental nature of the research and the fact that all tumors obtained are archival tissues at AFIP the investigators will not report the result of gene expression back to the subject
To date all samples needed for the protocol have been reached and there will be no more patient accrual but survival status of the patients as well as molecular analysis using the constructed lung tissue microarrays will continue for the foreseeable future
To date all samples needed for the protocol have been reached and there will be no more patient accrual but survival status of the patients as well as molecular analysis using the constructed lung tissue microarrays will continue for the foreseeable future
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 01-C-N233 | None | None | None |