Ignite Creation Date:
2024-05-05 @ 4:53 PM
Last Modification Date:
2024-10-26 @ 9:25 AM
Study NCT ID:
NCT00339287
Status:
RECRUITING
Last Update Posted:
2024-07-01
First Post:
2006-06-19
Brief Title:
Molecular Basis of Human Phagocyte Interactions With Bacterial Pathogens
Sponsor:
National Institute of Allergy and Infectious Diseases NIAID
Organization:
National Institutes of Health Clinical Center CC
Study Overview
Official Title:
Molecular Basis of Human Phagocyte Interactions With Bacterial Pathogens
Status:
RECRUITING
Status Verified Date:
2024-08-13
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Human phagocytic cells such as polymorphonuclear leukocytes PMNs are readily mobilized to sites of infection and ingest microorganisms by a process known as phagocytosis The combined effects of reactive oxygen species ROS and proteolytic peptides and enzymes released into forming bacterial phagosomes kill most ingested bacteria However many human bacterial pathogens have devised means to subvert normal phagocyte responses and the innate immune response and cause severe disease
The overall objective of this study is to elucidate specific features of pathogen-phagocyte interactions that underlie evasion of the innate immune response or contribute to the pathophysiology of disease or inflammatory disorders Therefore specific projects will
1 Identify and characterize specific mechanisms used by pathogenic microorganisms to evade or subvert normal phagocyte responses and therefore cause disease
2 Investigate phagocyte response mechanisms to specific pathogenic microorganisms
3 Identify specific bacterial structures andor gene products that dictate differences in phagocyte responses among a range of pathogens so that generalized statements can be made about the pathophysiology of disease states
The studies will be performed using multiple techniques including state-of-the-art equipment for genomics and proteomics strategies to identify target bacterial genesproteins of interest or those up-regulated in phagocytes Phagocyte-pathogen interactions will be examined using fluorescence-based real-time assays and video microscopy confocal and electron microscopy in combination with enzymatic assays for ROS production routine biochemistry immunology and cell biology
Implementing these studies will require isolation of phagocytic leukocytes from venous blood of healthy human volunteers The study population will be all-inclusive except in certain instances where individuals possess genetic defects that impair phagocyte function eg myeloperoxidase-deficiency or have altered phagocyte function due to outside influences such as recent bacterial or viral infection
The proposed studies will likely provide new information pertinent to understanding host cell-pathogen interactions and the pathophysiology of inflammatory conditions
The overall objective of this study is to elucidate specific features of pathogen-phagocyte interactions that underlie evasion of the innate immune response or contribute to the pathophysiology of disease or inflammatory disorders Therefore specific projects will
1 Identify and characterize specific mechanisms used by pathogenic microorganisms to evade or subvert normal phagocyte responses and therefore cause disease
2 Investigate phagocyte response mechanisms to specific pathogenic microorganisms
3 Identify specific bacterial structures andor gene products that dictate differences in phagocyte responses among a range of pathogens so that generalized statements can be made about the pathophysiology of disease states
The studies will be performed using multiple techniques including state-of-the-art equipment for genomics and proteomics strategies to identify target bacterial genesproteins of interest or those up-regulated in phagocytes Phagocyte-pathogen interactions will be examined using fluorescence-based real-time assays and video microscopy confocal and electron microscopy in combination with enzymatic assays for ROS production routine biochemistry immunology and cell biology
Implementing these studies will require isolation of phagocytic leukocytes from venous blood of healthy human volunteers The study population will be all-inclusive except in certain instances where individuals possess genetic defects that impair phagocyte function eg myeloperoxidase-deficiency or have altered phagocyte function due to outside influences such as recent bacterial or viral infection
The proposed studies will likely provide new information pertinent to understanding host cell-pathogen interactions and the pathophysiology of inflammatory conditions
Detailed Description:
Human phagocytic cells such as polymorphonuclear leukocytes neutrophils or PMNs are readily mobilized to sites of infection and ingest microorganisms by a process known as phagocytosis The combined effects of reactive oxygen species ROS and antimicrobial peptides released into forming bacterial phagosomes kill most ingested bacteria However many bacterial pathogens have devised means to evade normal phagocyte responses and cause severe disease in humans
The overall objective of this study is to elucidate specific features of pathogen-phagocyte interactions that underlie evasion of the innate immune response or contribute to the pathophysiology of disease or inflammatory disorders Therefore projects will address 3 specific aims
1 Identify and characterize specific mechanisms used by pathogenic microorganisms to evade or subvert normal phagocyte responses and therefore cause disease
2 Investigate phagocyte response mechanisms to specific pathogenic microorganisms
3 Identify specific bacterial structures andor gene products that dictate differences in phagocyte responses among a range of pathogens so that generalized statements can be made about the pathophysiology of disease states
The studies will be performed using multiple techniques including state-of-the-art equipment for genomics and proteomics strategies to identify target bacterial genesproteins of interest or those up-regulated in phagocytes Phagocyte-pathogen interactions will be examined using fluorescence-based real-time assays and video microscopy confocal and electron microscopy in combination with enzymatic assays for ROS production routine biochemistry immunology and cell biology
Implementing these studies will require isolation of phagocytic leukocytes from venous blood of healthy human volunteers The study population will be all-inclusive except in certain instances where individuals possess genetic defects that impair phagocyte function eg myeloperoxidase-deficiency or have altered phagocyte function due to outside influences such as recent bacterial or viral infection
The proposed studies will likely provide new information pertinent to understanding host cell-pathogen interactions and the pathophysiology of inflammatory conditions
The overall objective of this study is to elucidate specific features of pathogen-phagocyte interactions that underlie evasion of the innate immune response or contribute to the pathophysiology of disease or inflammatory disorders Therefore projects will address 3 specific aims
1 Identify and characterize specific mechanisms used by pathogenic microorganisms to evade or subvert normal phagocyte responses and therefore cause disease
2 Investigate phagocyte response mechanisms to specific pathogenic microorganisms
3 Identify specific bacterial structures andor gene products that dictate differences in phagocyte responses among a range of pathogens so that generalized statements can be made about the pathophysiology of disease states
The studies will be performed using multiple techniques including state-of-the-art equipment for genomics and proteomics strategies to identify target bacterial genesproteins of interest or those up-regulated in phagocytes Phagocyte-pathogen interactions will be examined using fluorescence-based real-time assays and video microscopy confocal and electron microscopy in combination with enzymatic assays for ROS production routine biochemistry immunology and cell biology
Implementing these studies will require isolation of phagocytic leukocytes from venous blood of healthy human volunteers The study population will be all-inclusive except in certain instances where individuals possess genetic defects that impair phagocyte function eg myeloperoxidase-deficiency or have altered phagocyte function due to outside influences such as recent bacterial or viral infection
The proposed studies will likely provide new information pertinent to understanding host cell-pathogen interactions and the pathophysiology of inflammatory conditions
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 01-I-N055 | None | None | None |