Ignite Creation Date:
2024-05-05 @ 11:08 AM
Last Modification Date:
2024-10-26 @ 9:05 AM
Study NCT ID:
NCT00005374
Status:
COMPLETED
Last Update Posted:
2016-02-10
First Post:
2000-05-25
Brief Title:
Genetics of the Metabolic Syndrome in Japanese Americans
Sponsor:
University of Washington
Organization:
University of Washington
Study Overview
Official Title:
None
Status:
COMPLETED
Status Verified Date:
2005-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
To investigate the genetic influence of candidate gene polymorphisms on risk factors for the metabolic insulin resistance syndrome in Japanese American sibships and kindreds The original grant in 1994 had as its objective to understand the genetic epidemiology of coronary heart disease CHD risk factors in Japanese- American families with probands living in Seattle Washington
Detailed Description:
BACKGROUND
Although each of the risk factors have recently been associated with increased risk of CHD and are known to be genetically influenced none of them have been investigated in a large sample of American families of Japanese ancestry The project represents a unique and timely opportunity to characterize the genetic epidemiology of CHD risk factors among Japanese Americans The findings could lead to the development of effective preventive strategies targeted to subgroups of individuals with high risk due to underlying genetic susceptibility
DESIGN NARRATIVE
In the original study several hypotheses were tested including 1 that a predominance of small LDL particles ALP phenotype B as determined by gradient gel electrophoresis was inherited as a single gene trait in Japanese-American kindreds and to compare these results with previous studies in Caucasian families 2 that ALP-B was associated with risk factors characteristic of the insulin resistance syndrome and NIDDM among individual Japanese-American family members 3 that plasma levels of Lpa were inversely associated with apoa size phenotypes as determined by high- resolution SDS-agarose-gel electrophoresis followed by immunoblotting in individual Japanese-American subjects and to compare and contrast these associations with those previously reported in Caucasians and other ethnic groups 4 that in addition to apoa gene effects the segregation of plasma levels of Lpa in families was nherited consistent with the presence of another single major gene effect The study also established a repository of frozen white cells for future genetic studies of candidate genes associated with risk of CHD in Japanese Americans These hypotheses were all tested based on blood samples blood pressure and anthropometric measurements and questionnaire data from 33 Japanese-American kindreds identified through participants in the ongoing Japanese-American Community Diabetes Study in Seattle The kindreds consist of 126 nuclear families and 443 individual family members including probands siblings spouses offspring and nieces and nephews of full Japanese descent
In 1998 the renewal has three specific aims The first aim was to identify genetic influences on the risk factors that characterize ther metabolic insulin resistance syndrome including fasting insulin proinsulin C-peptide and glucose body weight and waist circumference lipoproteins blood pressure fibrinogen factor VII and plasminogen activator inhibitor Statistical genetic analysis approaches used in the first aim included univariate complex segregation analysis factor analysis and quantitative multivariate genetic analysis The second specific aim was to test for genetic linkage between specific candidate genes involved in lipid metabolism carbohydrate metabolism blood pressure obesity and hemostasis with genetically influenced risk factors of the metabolic syndrome in Japanese-Americans The third specific aim was when the DNA repository had been completed 750 samples by the end of year four to apply to the NHLBI Mammalian Genotyping Service to perform a whole genome screen to identify new genes involved in susceptibility to the metabolic syndrome
Although each of the risk factors have recently been associated with increased risk of CHD and are known to be genetically influenced none of them have been investigated in a large sample of American families of Japanese ancestry The project represents a unique and timely opportunity to characterize the genetic epidemiology of CHD risk factors among Japanese Americans The findings could lead to the development of effective preventive strategies targeted to subgroups of individuals with high risk due to underlying genetic susceptibility
DESIGN NARRATIVE
In the original study several hypotheses were tested including 1 that a predominance of small LDL particles ALP phenotype B as determined by gradient gel electrophoresis was inherited as a single gene trait in Japanese-American kindreds and to compare these results with previous studies in Caucasian families 2 that ALP-B was associated with risk factors characteristic of the insulin resistance syndrome and NIDDM among individual Japanese-American family members 3 that plasma levels of Lpa were inversely associated with apoa size phenotypes as determined by high- resolution SDS-agarose-gel electrophoresis followed by immunoblotting in individual Japanese-American subjects and to compare and contrast these associations with those previously reported in Caucasians and other ethnic groups 4 that in addition to apoa gene effects the segregation of plasma levels of Lpa in families was nherited consistent with the presence of another single major gene effect The study also established a repository of frozen white cells for future genetic studies of candidate genes associated with risk of CHD in Japanese Americans These hypotheses were all tested based on blood samples blood pressure and anthropometric measurements and questionnaire data from 33 Japanese-American kindreds identified through participants in the ongoing Japanese-American Community Diabetes Study in Seattle The kindreds consist of 126 nuclear families and 443 individual family members including probands siblings spouses offspring and nieces and nephews of full Japanese descent
In 1998 the renewal has three specific aims The first aim was to identify genetic influences on the risk factors that characterize ther metabolic insulin resistance syndrome including fasting insulin proinsulin C-peptide and glucose body weight and waist circumference lipoproteins blood pressure fibrinogen factor VII and plasminogen activator inhibitor Statistical genetic analysis approaches used in the first aim included univariate complex segregation analysis factor analysis and quantitative multivariate genetic analysis The second specific aim was to test for genetic linkage between specific candidate genes involved in lipid metabolism carbohydrate metabolism blood pressure obesity and hemostasis with genetically influenced risk factors of the metabolic syndrome in Japanese-Americans The third specific aim was when the DNA repository had been completed 750 samples by the end of year four to apply to the NHLBI Mammalian Genotyping Service to perform a whole genome screen to identify new genes involved in susceptibility to the metabolic syndrome
Study Oversight
Has Oversight DMC:
Is a FDA Regulated Drug?:
Is a FDA Regulated Device?:
Is an Unapproved Device?:
Is a PPSD?:
Is a US Export?:
Is an FDA AA801 Violation?:
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL050268 | NIH | None | httpsreporternihgovquickSearchR01HL050268 |