Ignite Creation Date:
2024-05-05 @ 4:51 PM
Last Modification Date:
2024-10-26 @ 9:24 AM
Study NCT ID:
NCT00324207
Status:
COMPLETED
Last Update Posted:
2010-03-05
First Post:
2006-05-08
Brief Title:
Molecular Quantification of Insulin mRNA
Sponsor:
Childrens Mercy Hospital Kansas City
Organization:
Childrens Mercy Hospital Kansas City
Study Overview
Official Title:
Prediction of Type 1 Diabetes Molecular Quantification of Insulin mRNA in the While Cell Fraction of Whole Blood
Status:
COMPLETED
Status Verified Date:
2010-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The objectives of this study are to
1 quantitate the level of insulin mRNA in the while cell fraction of whole blood in selected groups of subjects
2 determine the gene expression of markers of white cell activation in the white cell fraction of whole blood in selected subjects
3 determine the identity of the white cell responsible for expression of insulin mRNA and
4 determine protein expression of mRNA of insulinproinsulin and selected genes of white cell activation
1 quantitate the level of insulin mRNA in the while cell fraction of whole blood in selected groups of subjects
2 determine the gene expression of markers of white cell activation in the white cell fraction of whole blood in selected subjects
3 determine the identity of the white cell responsible for expression of insulin mRNA and
4 determine protein expression of mRNA of insulinproinsulin and selected genes of white cell activation
Detailed Description:
Indicators of active immune or chemically mediated beta cell destruction would be helpful for predicting the development and severity of autoimmune diabetes and monitoring the success of islet transplants Currently there are no reliable indicators readily available
Insulin mRNA in the white cell fraction of blood has been detected or elevated under several circumstances including
1 normal animals
2 after islet cell transplantation
3 hyperglycemic induced expression and
4 during induction of diabetes with streptozotocin
The investigators propose that quantitative measurement of insulin mRNA may be an accurate innovative and minimally invasive indicator of beta cell destruction
Insulin mRNA in the white cell fraction of blood has been detected or elevated under several circumstances including
1 normal animals
2 after islet cell transplantation
3 hyperglycemic induced expression and
4 during induction of diabetes with streptozotocin
The investigators propose that quantitative measurement of insulin mRNA may be an accurate innovative and minimally invasive indicator of beta cell destruction
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 00006028 | None | None | None |