Ignite Creation Date:
2024-05-06 @ 12:14 PM
Last Modification Date:
2024-10-26 @ 12:56 PM
Study NCT ID:
NCT03711838
Status:
COMPLETED
Last Update Posted:
2022-12-19
First Post:
2018-10-11
Brief Title:
Redox Regulation of Satellite Cells and Skeletal Muscle Healing
Sponsor:
University of Thessaly
Organization:
University of Thessaly
Study Overview
Official Title:
The Effect of Redox Potential on the Regulation of Satellite Cells and Skeletal Muscle Healing Following Exercise-Induced Muscle Damage
Status:
COMPLETED
Status Verified Date:
2022-12
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Skeletal muscle stem cells Satellite cells are indispensable for muscle growth and remodeling following myofibril damage Skeletal muscle trauma is present in numerous catabolic conditions characterized by elevated proteolysis and muscle wasting such as cancer cachexia and muscular dystrophy which result in physical capacity impairment and a deteriorated quality of life Recent studies performed in animals and cell cultures indicate that the increased levels of inflammation and oxidative stress and the reduction of antioxidant defense may blunt the satellite cells response and myogenic programming during muscle healing However evidence regarding the effects of redox status on satellite cells and muscle myogenic potential in humans is lacking Exercise-induced muscle damage bears striking similarities with the aforementioned conditions which makes it a valuable tool to investigate the redox-dependent regulation of satellite cells during muscle healing Thus the objectives of the present study are to examine the effects of redox status perturbation via N-acetylcysteine administration on intracellular pathways responsible for satellite cells responses at rest and following aseptic muscle trauma induced by damaging exercise
Detailed Description:
A total number of 40-60 males young individuals aged 18-30 years will be initially enrolled in the study Then participants will be allocated to either a 1 Low Respondents LR or a 2 High Respondents HR group based on the basal satellite cells content of their vastus lateralis muscle of their dominant leg In a double-blind crossover repeated measures design participants will consume either Placebo PLA or N-acetylcysteine NAC before 7-day loading phase on exercise day and for 8 consecutive days following a single bout of intense exercise 300 eccentric contractions at 30 degsec in an isokinetic dynamometer In both conditions blood samples and muscle biopsies will be collected at baseline before the exercise protocol and at 2- and 8-days post-exercise Muscle performance and soreness will also be assessed at the same time points Before the first trial participants dietary intake via diet recalls and physical activity via accelerometry will be analyzed and nutritional plans will be provided to participants in order to normalize their antioxidant and protein intake A 4-week washout period will be implemented between trials Blood samples will be analyzed for inflammation and oxidative stress markers Muscle samples will be analyzed for satellite cells responses and myogenic potential protein levels of intracellular signaling proteins muscle thiols and antioxidant enzymes activity
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None