Ignite Creation Date:
2024-05-06 @ 12:06 PM
Last Modification Date:
2024-10-26 @ 12:54 PM
Study NCT ID:
NCT03686111
Status:
UNKNOWN
Last Update Posted:
2018-09-26
First Post:
2018-09-25
Brief Title:
NUCLEAR LAMINA OVARIAN AGE AND MEDICAL ASSISTANCE TO PROCREATION
Sponsor:
Assistance Publique Hopitaux De Marseille
Organization:
Assistance Publique Hopitaux De Marseille
Study Overview
Official Title:
NUCLEAR LAMINA OVARIAN AGE AND MEDICAL ASSISTANCE TO PROCREATION
Status:
UNKNOWN
Status Verified Date:
2018-09
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The nuclear lamina LN consists of a network of proteins interposed between the chromatin and the internal nucleic membrane to which it is closely associated LN consists essentially of lamins encoded by the LMNA Lamines A C LMNB1 and LMNB2 genes BI B2 B3 lamins Malfunctions of the A C-type laminates causing the production of a toxic isoform called progerin are directly responsible for various serious pathologies such as Progeria Hutchingson-Gilford This rare disease is characterized by accelerated pathological aging Moreover it is known that progerin is produced in the absence of any mutation of the gene LMNA is involved in the physiological aging of tissus
The ovarian reserve of a woman is a parameter that changes with age Although this progressive loss is inevitable in the course of life the kinetics of this ovarian exhaustion appear variable according to the women sometimes leading to an accelerated loss causing a discordance between the female age and the ovarian age But ovarian age becomes a major prognostic parameter to be taken into account during any attempt at medical assistance to procreation AMP and is often incriminated in the failures of MPAs There are currently few markers to predict the quality of ovarian response of women to ovarian stimulation let alone the quality of the oocytes retrieved However their quality directly influences their ability to give an evolving embryo and therefore a pregnancy Our team has previously shown that type B laminates are involved in human spermiogenesis and highlighted the human B3 isoform These very encouraging results obtained on human spermatogenesis have prompted us to also look at the potential interest of the exploration of the nuclear lamina within the human ovary
The aim of this project is therefore to continue the exploration of the nuclear lamina and its potential role on the quality of human gametes by studying the follicular ovarian cells surrounding the oocyte These cells express A C type lamins and sometime progerin Investigators want to conduct a prospective pilot study in a cohort of 40 women in MPA aged between 20 and 42 years The main objective is to compare the levels of expression of the A C B and progerin laminae in the follicular cells of 2 groups of women a group of infertile patients managed to induce ovulation in order to Intraconjugal AMP and a group of women managed to induce ovulation to give their oocytes For this purpose investigators will analyze the expression of the A C and B lamins in RT-PCR the localization of the lamins and progerin in immunofluorescence and will look for quantitative variations in the production of A C and or progerin lamins by western- blot depending on the age of the patients
This pilot study will allow investigators to characterize for the first time the LN of human follicular cells They should highlight variations in the expression and or localization of lamins and possibly progerin in patients tested according to their age and or ovarian reserve The team preliminary results showed that this track deserves to be explored This is an excellent opportunity to bring new insights into the understanding of the mechanisms responsible for alteration of ovarian reserve in women and the prognosis of MPA attempts
Finally this approach could serve as a basis for a larger multicenter study These markers could represent new diagnostic markers to be taken into account to evaluate the chances of pregnancy of each woman in AMP
The ovarian reserve of a woman is a parameter that changes with age Although this progressive loss is inevitable in the course of life the kinetics of this ovarian exhaustion appear variable according to the women sometimes leading to an accelerated loss causing a discordance between the female age and the ovarian age But ovarian age becomes a major prognostic parameter to be taken into account during any attempt at medical assistance to procreation AMP and is often incriminated in the failures of MPAs There are currently few markers to predict the quality of ovarian response of women to ovarian stimulation let alone the quality of the oocytes retrieved However their quality directly influences their ability to give an evolving embryo and therefore a pregnancy Our team has previously shown that type B laminates are involved in human spermiogenesis and highlighted the human B3 isoform These very encouraging results obtained on human spermatogenesis have prompted us to also look at the potential interest of the exploration of the nuclear lamina within the human ovary
The aim of this project is therefore to continue the exploration of the nuclear lamina and its potential role on the quality of human gametes by studying the follicular ovarian cells surrounding the oocyte These cells express A C type lamins and sometime progerin Investigators want to conduct a prospective pilot study in a cohort of 40 women in MPA aged between 20 and 42 years The main objective is to compare the levels of expression of the A C B and progerin laminae in the follicular cells of 2 groups of women a group of infertile patients managed to induce ovulation in order to Intraconjugal AMP and a group of women managed to induce ovulation to give their oocytes For this purpose investigators will analyze the expression of the A C and B lamins in RT-PCR the localization of the lamins and progerin in immunofluorescence and will look for quantitative variations in the production of A C and or progerin lamins by western- blot depending on the age of the patients
This pilot study will allow investigators to characterize for the first time the LN of human follicular cells They should highlight variations in the expression and or localization of lamins and possibly progerin in patients tested according to their age and or ovarian reserve The team preliminary results showed that this track deserves to be explored This is an excellent opportunity to bring new insights into the understanding of the mechanisms responsible for alteration of ovarian reserve in women and the prognosis of MPA attempts
Finally this approach could serve as a basis for a larger multicenter study These markers could represent new diagnostic markers to be taken into account to evaluate the chances of pregnancy of each woman in AMP
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 2017-A01258-45 | OTHER | ANSM | None |