Ignite Creation Date:
2024-05-05 @ 4:46 PM
Last Modification Date:
2024-10-26 @ 9:24 AM
Study NCT ID:
NCT00319059
Status:
COMPLETED
Last Update Posted:
2016-04-22
First Post:
2006-04-27
Brief Title:
The Influence of the 34CT Variant in the AMPD1 Gene Ischemic Tolerance
Sponsor:
Radboud University Medical Center
Organization:
Radboud University Medical Center
Study Overview
Official Title:
The Influence of the 34CT Variant in the AMPD1 Gene on Individual Susceptibility for Ischemia-reperfusion
Status:
COMPLETED
Status Verified Date:
2016-04
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Previous epidemiological studies have shown that in cardiovascular patients the 34CT variant in the gene encoding for the enzyme Adenosine Mono Phosphate Deaminase AMPD1 is associated with prolonged survival
The 34 CT variant encodes a severely truncated metabolically inactive protein We hypothesize that during ischemia in these patients AMP in preferentially converted into adenosine instead of IMP Adenosine receptor stimulation in turn will increase resistance to ischemia-reperfusion in the myocardial tissue
To test this hypothesis 7 male healthy volunteers heterozygous for the 34CT variant will be selected from 100 healthy volunteers which we have previously genotyped These subjects will be compared with 7 matched control subjects Individual ischemic tolerance will be assessed in the thenar muscle using 99mTc-Annexin A5 scintigraphy
Briefly the circulation of the nondominant forearm will be interrupted for 10 minutes by inflation of an upperarm cuff to 200mmHg en concomitantly the subjects will perform isometric rhythmic handgripping until exhaustion Immediately upon reperfusion 400 MBq of 99mTc-Annexin A5 will be administered intravenously Finally 1 and 4 hours post-injection scintigrapghi imaging of both hand will be performed Targeting of annexin A5 will be expressed as percentage difference between the experimental and control hand
The 34 CT variant encodes a severely truncated metabolically inactive protein We hypothesize that during ischemia in these patients AMP in preferentially converted into adenosine instead of IMP Adenosine receptor stimulation in turn will increase resistance to ischemia-reperfusion in the myocardial tissue
To test this hypothesis 7 male healthy volunteers heterozygous for the 34CT variant will be selected from 100 healthy volunteers which we have previously genotyped These subjects will be compared with 7 matched control subjects Individual ischemic tolerance will be assessed in the thenar muscle using 99mTc-Annexin A5 scintigraphy
Briefly the circulation of the nondominant forearm will be interrupted for 10 minutes by inflation of an upperarm cuff to 200mmHg en concomitantly the subjects will perform isometric rhythmic handgripping until exhaustion Immediately upon reperfusion 400 MBq of 99mTc-Annexin A5 will be administered intravenously Finally 1 and 4 hours post-injection scintigrapghi imaging of both hand will be performed Targeting of annexin A5 will be expressed as percentage difference between the experimental and control hand
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None