Ignite Creation Date:
2024-05-06 @ 11:41 AM
Last Modification Date:
2024-10-26 @ 12:48 PM
Study NCT ID:
NCT03577093
Status:
UNKNOWN
Last Update Posted:
2018-07-05
First Post:
2018-06-22
Brief Title:
Molecular Mechanisms of microRNA-494 Involving in Cerebral Ischemia
Sponsor:
Capital Medical University
Organization:
Capital Medical University
Study Overview
Official Title:
Study on the Molecular Mechanism of miR-494 Mediating Cell Cycle Regulation Following Cerebral Ischemia
Status:
UNKNOWN
Status Verified Date:
2018-07
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
We and other investigations suggested that the activation of nerve cell cycle following cerebral ischemia led to neuronal apoptosis glial cell proliferation and the formation of glial scarThe cyclin-dependent kinases CDKs and cyclins jointly promoted the cell cycle progression Our preliminary clinical trial found a new microRNA-miR-494 which involved in the occurrence of acute ischemic stroke In our animal experiment miR-494 could relieve cerebral ischemia injury through inhibiting cyclin-dependent kinase 6CDK6 ubiquitin-conjugating enzyme E2L6 UBE2L6 and histone deacetylase 3 HDAC3 which suggested that miR-494 might play an important role in the regulation of cell cycle following cerebral ischemia This project intends to verify the following hypothesis①miR-494 suppresses CDK6 andor fibroblast growth factor16FGF16-Ras-extracellular signal-regulated kinaseERK--v-myc avian myelocytomatosis viral oncogene homologMYC pathway andor phosphatase and tensin homologPTEN-protein kinase BAKT-mechanistic target of rapamycinmTOR-S6k pathway②miR-494 inhibits UBE2L6 upregulates the hypoxia-inducible factor 1 αHIF-1α expression in nerve cells thereby increases the p21 and p27 protein levels and inhibits cyclin-dependent kinase2CDK2activity③miR-494 represses HDAC3 and downregulates the cyclin-dependent kinase1CDK1protein level These all mediate the cell cycle arrest of neurons and astrocytes reduce the neuronal apoptosis and glial scar formationpromote the recovery of neurological function and provide new targets for the treatment of ischemic stroke
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None