Ignite Creation Date:
2024-05-06 @ 11:21 AM
Last Modification Date:
2024-10-26 @ 12:44 PM
Study NCT ID:
NCT03497052
Status:
UNKNOWN
Last Update Posted:
2018-04-24
First Post:
2018-04-06
Brief Title:
Culture Media and Blastocyst Development
Sponsor:
Clinica Valle Giulia
Organization:
Clinica Valle Giulia
Study Overview
Official Title:
Blastocyst Developmental Rate in Two Different Single Step Culture Media a Sibling Oocyte Prospective Non-interventional Study
Status:
UNKNOWN
Status Verified Date:
2018-04
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The aim of this non-interventional study is to compare the blastulation rate per fertilized oocyte in two different single step culture media commercially available GEMS and Irvine culture media
Detailed Description:
SUMMARY
In ART procedures blastocyst transfer can be considered the most physiological situation as the embryo is placed in the uterine cavity at a stage most similar to that which occurs in nature These potential benefits have induced more and more centres to move from cleavage stage to blastocyst stage embryo transfer all over the world Maheshwari et al 2016 Despite this there are also some potential disadvantages This approach decreases the total number of usable embryos defined as those available for transfer or cryopreservation Glujovsky et al 2016 Some concerns regarding the safety of this approach have also been raised In particular the extended duration of the in vitro culture may have long-term effect in the embryo development In a recent meta-analysis blastocyst stage transfer was in fact associated with increased risks of preterm birth 37 weeks very preterm birth 32 weeks large for gestational age and perinatal mortality although the latter was only identified from one study Conversely blastocyst transfer was associated with a decrease in the risk of small for gestational age and vanishing twins although this latter was reported by only one study Martins et al 2017
Culture media plays a crucial role in this context Two distinct approaches aimed at supporting embryo development to the blastocyst stage have been proposed the back to nature sequential approach and the let the embryo choose single medium approach Summers et al 2003 Machtinger et al 2012 Quinn 2012
Sequential culture media system is designed to meet identified changing metabolic and nutritional requirements of the developing embryo from day 1 to day 5 Gardner et al 1996 1997 1998 1999 With this approach embryos are grown up to day 3 in a first medium and then at the cleavage stage are moved to a second one The goal is to expose the embryos to stage-specific media designed to reflect observed changes in concentrations of pyruvate lactate and glucose in the Fallopian tube versus the uterus Gardner et al 1996
On the other hand single culture media aim at allowing developing embryos to choose the nutrients they require while at the same time minimizing stress from exposure to an abrupt change in their culture environment on day 3 Single media can be used with a medium change on day 3 or in a single-step uninterrupted culture in which there is no medium refreshing on day 3 Biggers et al 2008 Available data comparing the performance of single vs sequential culture media suggest that both types of media seem to provide similar support to the developing embryo Sfontouris et al 2017
Aim of the study and design
The aim of this non-interventional study is to compare the blastulation rate per fertilized oocyte in two different single step culture media commercially available GEMS and Irvine culture media
Oocyte collection
Consecutive egg collection will be performed with inclusion of oocytes coming from woman not older than 42 years of age presenting between 4 and 8 normal appearing MII oocytes and undergoing ICSI treatment with ejaculated sperm in the Centre for Reproductive Medicine GENERA in Rome To exclude potential negative paternal effect on blastulation rate all oocytes coming from couples with surgically extracted spermatozoa and very severe oligoastenoteratozoospermia motile sperm count 500000ml after preparation will be excluded Oocytes coming from patients enrolled in PGD program for monogenic diseases or structural chromosomal abnormalities will be excluded too
It is estimated based centre experience the study will last for 18 months The informed consent is the GENERA standard format in ordinary use The study will be approved by the Institutional Review Board of the Clinic
Oocyte denudation evaluation and injection All the procedures will be performed according to standard practice without any kind of intervention
Just after the pick-up procedure oocytes will be denuded from the cumulus oophorus by a brief exposure to 40 IUml hyaluronidase solution followed by mechanical removal of the corona radiata with the use of plastic pipettes of defined diameters in a controlled temperature environment This procedure will be performed between 37 and 40 hours post hCG administration Metaphase II MII oocytes will be then separated from the immature oocytes and evaluated at the stereomicroscope Those with severe morphological abnormalities will be considered of lower quality according to Rienzi et al 2008 and will thus be excluded from comparison All the MII oocyte will be placed in the same Petri Dish
Using randomization tables oocytes from each patient will be randomly split in two groups that will be independently inseminated and subsequently kept in 2 single step culture media GEMS GERI Medium and Irvine Continuous Single Culture Complete with HSA Oocytes will be subjected to ICSI using previously described techniques and instrumentations Rienzi et al 1998 To be able to follow the developmental progression of individual oocyte inseminated oocytes from each group will be cultured in separate dishes and each individual oocyte will be identified according to its position within the dish microwells Embryo culture will last up to day 5 and will be performed in a time-lapse incubator GERI Genea in hypoxic atmosphere containing 6CO2 and 5O2
Embryo assessment
As per GENERA procedure detailed analysis of various events during embryo development syngamy cleavages compaction and blastulation will be assessed using morphokinetic analysis Various parameters will be assessed time between the end of the ICSI procedure and syngamy completion of cleavage to 2 3 4 5 and 8 cells T2 T3 T4 T5 T8 respectively length of the first second and third cell cycle CC1 CC2 and CC3 respectively and synchrony in the division from three to four and five to eight cells S2 and S3 respectively Standard blastocyst morphological assessment will be performed according to the criteria reported by Gardner and Schoolcraft 1999 In brief the blastocysts will be evaluated according to the degree of expansion quality of the inner cell mass ICM and of the trophectoderm cells TE The ICM is evaluated according to the number of cells and the degree of compaction while the TE is evaluated according to the number dimension of the cells and the appearance of the epithelium cohesive or loose Morphokinetic parameters related to blastocyst formation will also be recorded and in particular initiation of compaction initiation of blastulation and completion of blastulation TSC TSB TB respectively
Statistical Considerations The primary study endpoint is the blastulation rate computed as the percentage of fertilized eggs cultured in either medium that develop to the blastocyst stage in each half of the cohort Since only women with between 4 and 8 retrieved oocytes will be eligible and oocytes from each woman will be randomly split in 2 groups assuming a fertilization rate of 80 the study population to be analysed will be composed by X2 where X is the number of enrolled women groups of 2 oocytes half cultured in one medium and the other cultured in the second medium The most obvious approach to the analysis of the resulting data is to consider it as a set of X number of women 2x2 tables where the quantity to be estimated is the odds ratio of blastulation of one medium relative to the other to be analysed by means of the Mantel Haenszel procedure with woman as the stratification factor primary analysis Assuming an overall blastulation rate of 34 a minimal relevant difference of 7 corresponds to an odds ratio of blastulation of 14 In order to detect with alfa5 2-sided and power 80 an odds ratio of 14 approximately 1200 1182 oocytes need to be included Hulley et al 2013 split in 2 groups of equal sizes that correspond approximately to 160 - 200 women aprox 6 oocyteswoman retrieved and considering a fertilization rate 80
In secondary exploratory analysis a logistic model will be fitted to the data with blastulation as the dependent binary variable and woman as stratum to assess the predictive value of various prognostic factors and the possible interactions between each of them and the culture medium
In ART procedures blastocyst transfer can be considered the most physiological situation as the embryo is placed in the uterine cavity at a stage most similar to that which occurs in nature These potential benefits have induced more and more centres to move from cleavage stage to blastocyst stage embryo transfer all over the world Maheshwari et al 2016 Despite this there are also some potential disadvantages This approach decreases the total number of usable embryos defined as those available for transfer or cryopreservation Glujovsky et al 2016 Some concerns regarding the safety of this approach have also been raised In particular the extended duration of the in vitro culture may have long-term effect in the embryo development In a recent meta-analysis blastocyst stage transfer was in fact associated with increased risks of preterm birth 37 weeks very preterm birth 32 weeks large for gestational age and perinatal mortality although the latter was only identified from one study Conversely blastocyst transfer was associated with a decrease in the risk of small for gestational age and vanishing twins although this latter was reported by only one study Martins et al 2017
Culture media plays a crucial role in this context Two distinct approaches aimed at supporting embryo development to the blastocyst stage have been proposed the back to nature sequential approach and the let the embryo choose single medium approach Summers et al 2003 Machtinger et al 2012 Quinn 2012
Sequential culture media system is designed to meet identified changing metabolic and nutritional requirements of the developing embryo from day 1 to day 5 Gardner et al 1996 1997 1998 1999 With this approach embryos are grown up to day 3 in a first medium and then at the cleavage stage are moved to a second one The goal is to expose the embryos to stage-specific media designed to reflect observed changes in concentrations of pyruvate lactate and glucose in the Fallopian tube versus the uterus Gardner et al 1996
On the other hand single culture media aim at allowing developing embryos to choose the nutrients they require while at the same time minimizing stress from exposure to an abrupt change in their culture environment on day 3 Single media can be used with a medium change on day 3 or in a single-step uninterrupted culture in which there is no medium refreshing on day 3 Biggers et al 2008 Available data comparing the performance of single vs sequential culture media suggest that both types of media seem to provide similar support to the developing embryo Sfontouris et al 2017
Aim of the study and design
The aim of this non-interventional study is to compare the blastulation rate per fertilized oocyte in two different single step culture media commercially available GEMS and Irvine culture media
Oocyte collection
Consecutive egg collection will be performed with inclusion of oocytes coming from woman not older than 42 years of age presenting between 4 and 8 normal appearing MII oocytes and undergoing ICSI treatment with ejaculated sperm in the Centre for Reproductive Medicine GENERA in Rome To exclude potential negative paternal effect on blastulation rate all oocytes coming from couples with surgically extracted spermatozoa and very severe oligoastenoteratozoospermia motile sperm count 500000ml after preparation will be excluded Oocytes coming from patients enrolled in PGD program for monogenic diseases or structural chromosomal abnormalities will be excluded too
It is estimated based centre experience the study will last for 18 months The informed consent is the GENERA standard format in ordinary use The study will be approved by the Institutional Review Board of the Clinic
Oocyte denudation evaluation and injection All the procedures will be performed according to standard practice without any kind of intervention
Just after the pick-up procedure oocytes will be denuded from the cumulus oophorus by a brief exposure to 40 IUml hyaluronidase solution followed by mechanical removal of the corona radiata with the use of plastic pipettes of defined diameters in a controlled temperature environment This procedure will be performed between 37 and 40 hours post hCG administration Metaphase II MII oocytes will be then separated from the immature oocytes and evaluated at the stereomicroscope Those with severe morphological abnormalities will be considered of lower quality according to Rienzi et al 2008 and will thus be excluded from comparison All the MII oocyte will be placed in the same Petri Dish
Using randomization tables oocytes from each patient will be randomly split in two groups that will be independently inseminated and subsequently kept in 2 single step culture media GEMS GERI Medium and Irvine Continuous Single Culture Complete with HSA Oocytes will be subjected to ICSI using previously described techniques and instrumentations Rienzi et al 1998 To be able to follow the developmental progression of individual oocyte inseminated oocytes from each group will be cultured in separate dishes and each individual oocyte will be identified according to its position within the dish microwells Embryo culture will last up to day 5 and will be performed in a time-lapse incubator GERI Genea in hypoxic atmosphere containing 6CO2 and 5O2
Embryo assessment
As per GENERA procedure detailed analysis of various events during embryo development syngamy cleavages compaction and blastulation will be assessed using morphokinetic analysis Various parameters will be assessed time between the end of the ICSI procedure and syngamy completion of cleavage to 2 3 4 5 and 8 cells T2 T3 T4 T5 T8 respectively length of the first second and third cell cycle CC1 CC2 and CC3 respectively and synchrony in the division from three to four and five to eight cells S2 and S3 respectively Standard blastocyst morphological assessment will be performed according to the criteria reported by Gardner and Schoolcraft 1999 In brief the blastocysts will be evaluated according to the degree of expansion quality of the inner cell mass ICM and of the trophectoderm cells TE The ICM is evaluated according to the number of cells and the degree of compaction while the TE is evaluated according to the number dimension of the cells and the appearance of the epithelium cohesive or loose Morphokinetic parameters related to blastocyst formation will also be recorded and in particular initiation of compaction initiation of blastulation and completion of blastulation TSC TSB TB respectively
Statistical Considerations The primary study endpoint is the blastulation rate computed as the percentage of fertilized eggs cultured in either medium that develop to the blastocyst stage in each half of the cohort Since only women with between 4 and 8 retrieved oocytes will be eligible and oocytes from each woman will be randomly split in 2 groups assuming a fertilization rate of 80 the study population to be analysed will be composed by X2 where X is the number of enrolled women groups of 2 oocytes half cultured in one medium and the other cultured in the second medium The most obvious approach to the analysis of the resulting data is to consider it as a set of X number of women 2x2 tables where the quantity to be estimated is the odds ratio of blastulation of one medium relative to the other to be analysed by means of the Mantel Haenszel procedure with woman as the stratification factor primary analysis Assuming an overall blastulation rate of 34 a minimal relevant difference of 7 corresponds to an odds ratio of blastulation of 14 In order to detect with alfa5 2-sided and power 80 an odds ratio of 14 approximately 1200 1182 oocytes need to be included Hulley et al 2013 split in 2 groups of equal sizes that correspond approximately to 160 - 200 women aprox 6 oocyteswoman retrieved and considering a fertilization rate 80
In secondary exploratory analysis a logistic model will be fitted to the data with blastulation as the dependent binary variable and woman as stratum to assess the predictive value of various prognostic factors and the possible interactions between each of them and the culture medium
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None