Ignite Creation Date:
2024-05-06 @ 11:20 AM
Last Modification Date:
2024-10-26 @ 12:44 PM
Study NCT ID:
NCT03498053
Status:
COMPLETED
Last Update Posted:
2018-04-13
First Post:
2018-01-10
Brief Title:
Smoking Topography Study 2018
Sponsor:
Maastricht University Medical Center
Organization:
Maastricht University Medical Center
Study Overview
Official Title:
Smoking Topography Study 2018
Status:
COMPLETED
Status Verified Date:
2018-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Rationale The World Health Organization Framework Convention on Tobacco Control WHO FCTC aims for a regulatory strategy including the regulation of the contents of tobacco products Article 9 Cigarette smoke includes more than 7000 chemicals which are harmful and cause tobacco-related diseases In the future regulation of these harmful cigarette constituents should be based on more chemical classes as the WHO suggested However in order to introduce such class-based regulation a scientific base is needed to define upper limits of allowed amounts of chemicals groups in cigarette smoke emissions and to ensure decreased harmful health effects due to cigarette smoking To date the causality between human exposure to specific cigarette smoke compounds and the harmful effects is unknown The first step in closing the gap in knowledge between cigarette smoke exposure and developing tobacco-related diseases includes a proper determination of human exposure to cigarette smoke chemicals This includes measuring smoking topography and inhalation Smoking topography is how the smoker smokes the cigarette puff volume duration flow etc The goal is to link smoking behavior to smoke exposure for 2 different cigarette brands The participants will smoke their normal brand Marlboro experimental day 1 after which they receive the low TNCO tar nicotine and carbon monoxide Marlboro Prime to smoke at home A week later the experimental day day 2 is repeated with this cigarette On the last experimental day day 3 the participants will smoke the Prime cigarette while the ventilation holes of this low-TNCO cigarette are taped Afterwards the personal smoking profiles of the participants and thus their individual exposures will be mimicked in the lab using machine smoking The observed smoking topography and inhalation parameters together give information about the exposure to smoke toxicants In addition this study is also designed to measure biomarkers of exposure in body fluids of smokers such as nicotine and the most abundant cigarette smoke chemicals and their metabolites
Objective We want to find out whether the individual habitudinal smoking topography of a smoker smoking his usual brand and the changes between cigarettes over the day can be compared to that of smoking a low-TNCO or high nicotine cigarette ie the Marlboro Prime and Red Sun In addition differences in inhalation patterns are investigated Next to that the exposure will be connected to the nicotine and carbon monoxide levels in blood andor urine Also smoke toxicants and metabolites in exhaled air saliva urine and blood of smokers are determined
Study design This prospective observational study monitors smokers in their habitudinal smoking during the day for 10 hr while smoking Marlboro Marlboro Prime and Marlboro Prime taped cigarette while during the day bodily fluids are sampled at several time points
Study population This population consists of 18 Caucasian healthy adult males aged between 25-34 years old Participants should be used to smoke Marlboro redregular for at least 3 years with a daily average of 13 to 25 cigarettes about a package every day
Nature and extent of the burden and risks associated with participation benefit and group relatedness The participating smokers smoke according to their habitudinal smoking pattern and are therefore not increasingly exposed to the harmful health effects of cigarette smoking The invasive part of the study is their stay for 3 days and 1 night when wanted in a hotel and the sampling of blood saliva urine and exhaled air
Objective We want to find out whether the individual habitudinal smoking topography of a smoker smoking his usual brand and the changes between cigarettes over the day can be compared to that of smoking a low-TNCO or high nicotine cigarette ie the Marlboro Prime and Red Sun In addition differences in inhalation patterns are investigated Next to that the exposure will be connected to the nicotine and carbon monoxide levels in blood andor urine Also smoke toxicants and metabolites in exhaled air saliva urine and blood of smokers are determined
Study design This prospective observational study monitors smokers in their habitudinal smoking during the day for 10 hr while smoking Marlboro Marlboro Prime and Marlboro Prime taped cigarette while during the day bodily fluids are sampled at several time points
Study population This population consists of 18 Caucasian healthy adult males aged between 25-34 years old Participants should be used to smoke Marlboro redregular for at least 3 years with a daily average of 13 to 25 cigarettes about a package every day
Nature and extent of the burden and risks associated with participation benefit and group relatedness The participating smokers smoke according to their habitudinal smoking pattern and are therefore not increasingly exposed to the harmful health effects of cigarette smoking The invasive part of the study is their stay for 3 days and 1 night when wanted in a hotel and the sampling of blood saliva urine and exhaled air
Detailed Description:
In 2005 the World Health Organization Framework Convention on Tobacco Control WHO FCTC was established with the aim for a regulatory strategy as a response to the globalization of the tobacco epidemic One of their non-price measures to reduce the demand for tobacco includes article 9 Regulation of the contents of tobacco products Cigarette product regulation is currently based on tar nicotine and carbon monoxide TNCO levels in cigarette smoke which are indicated on the package This is not sufficient since cigarette smoke includes more than 7000 chemicals There are aldehydes VOCs PAHs nitrosamines metals and so much more measured in cigarette causing tobacco-related diseases
In the future regulation of these harmful cigarette constituents should be based on more chemical classes as the WHO suggested However in order to introduce such class-based regulation a scientific base is needed to define upper limits of allowed amounts of chemicals groups in cigarette smoke emissions and to ensure decreased harmful effects due to cigarette smoking To date the causality between human exposure to specific cigarette smoke compounds and the harmful effects is unknown The first step in closing the gap in knowledge between cigarette smoke exposure and developing tobacco-related diseases includes a proper determination of human exposure to cigarette smoke chemicals
Unfortunately there is a lack of methodology to determine cigarette smoke exposure in humans
In a prospective observational pilot study in February 2016 natural human smoking behavior was characterized Smoking Topography Study 2016 NL5567606815 The smoking topography of every smoked Marlboro cigarette was monitored through the CRESSmicro device which records the puff length the puff interval the puff flow and the puff volume In this study the investigators could model the individual smoking profiles per participant by using data of 4 random cigarettes During modelling puff volume duration and thus also flow and interpuff interval were taken into account
Since there were no differences in smoking topography of cigarettes smoked observed at different times of the day implies that in this new study the researchers can invite participants for a shorter time because only data of 4 cigarettes is needed to adequately model their overall smoking profile needed for exposure measurements These smoking topography parameters are needed for the settings of a smoking machine applied to mimic the exact exposure to cigarette smoke toxicants for each individual
In our previous study the researchers concluded that smokers have their own individual smoking topography Of course smoking is also accompanied by the inhalation of harmful chemicals but the smoker is not aware of this during smoking and therefore does not adapt his smoking topography with respect to that exposure However it is unknown by what means the individual smoking topography parameters are related to toxicant exposure in the different parts of the lungs For example higher puff volumes with a different composition of toxicants lead to a different inhalation pattern than small puff volumes possible followed by different exposure in the lungs
In this new study the researchers want to include respiratory parameters of breathing and inhalation over the day as well Until now inhalation risk assessment is based on human breathing patterns while breathing and inhaling smoke are not the same due to the presence of harsh compounds in the smoke The current study aims to measure respiratory parameters to identify which lung compartments are exposed to what extent in relation to the observed smoking topography This can be achieved by respiratory inductive plethysmography RIP a non-invasive device that can be worn throughout the day In the current study the Hexoskin will be applied for these measurements
Literature describes that the process of smoking topography and inhalation differs per cigarette and situation In other words the smoker doses himself to gain nicotine with additional production of carbon monoxide CO and other harmful cigarette smoke-associated chemicals
As described before in the future cigarettes will be changed as they are only allowed to contain a maximum of certain toxicants One of the options to make cigarettes less addictive is to add less nicotine It is known that smokers show compensating behavior when smoking other cigarettes than their used to to gain the same amount of nicotine Our machine smoking experiments show that when smoking low-TNCO cigarettes the composition of the smoke per mg nicotine changes under the same smoking conditions This means that the cigarette characteristics are also at least partly responsible for the smoke composition The researchers are interested in how smoking behavior and inhalation and thus exposure changes when offering a smoker another type of cigarette
Therefore the researchers are interested whether the smoking behavior changes when the smoker has to smoke a low TNCO cigarette ie the Marlboro Prime The low values of nicotine in the smoke of the Marlboro Prime are partly achieved by ventilation holes in the cigarette filter By closing these filter vents with tape around the filter the smoke is no longer diluted with side stream air and the cigarette design is slightly different
The participants will smoke their normal brand Marlboro day 1 After the experimental day they receive the Marlboro Prime for smoking at home so they get used to smoking a new cigarette A week later the experimental day day 2 is repeated with this cigarette The participants can stay overnight or come back the next day day 3 to smoke the Prime cigarette while the ventilation holes of this low-TNCO cigarette are taped See the scheme in Table 1
As in the STS2016 this study again is aimed at measuring smoking in a habitual rhythm without imposing it as has been done thus far With this the natural smoking topography per cigarette can be measured in combination with the respiratory parameters by wearing a non-invasive RIP device By providing the participants with the Marlboro Prime cigarettes prior to the experimental days 2 and 3 they can get used to smoking them at home in order to develop natural smoking behavior
As during the last study the sampling is following a sampling scheme Figure 1 In summary during the day blood exhaled air urine and saliva will be sampled to measure nicotine carbon monoxide but also other cigarette smoke compounds such as aldehydes and their metabolites
In the future the personal smoking regimes of the participants and thus their personal exposures will be mimicked with machine smoking experiments This will be linked to the RIP parameters
Points of attention that came out of the STS 2016 include that smokers smoked less cigarettes than expected Reason for that is as they mentioned that they cannot smoke in the apartment while they smoke inside at home Another reason was that they have to smoke alone while at home or work there often is a co-smoker The participants didnt experience difficulties or changes in smoking behavior due to the use of CRESS They all practiced smoking with the CRESS the evening before the experimental day In the present study 2 smokers will be invited at the same time Again smokers have to go outside for a smoke to protect the researchers to secondhand smoke exposure This is kept as attainable as possible
Primary Objective
Is there a difference in natural smoking topography between Marlboro Marlboro Prime and Marlboro Prime taped leading to a different exposure for the smoker
Secondary Objectives
Is it possible to map personal smoking profiles with only 4 random cigarettes as showed in the previous Smoking Topography Study 2016
Is a different smoking topography profile per smoker related to deviating exposure of mainstream smoke in the respiratory tract compartments for the different cigarettes
Can exhaled air of smokers be used as biomarker for exposure to VOCsaldehydes
Are nicotine and toxicant metabolite levels in bloodurine changing over the day and connected to the smoking behavior of the different cigarettes
Study Design The Smoking Topography Study 2018 is a not-randomized cross-over study with a single group with the duration of 3 months Participants will visit our research apartment at Apart Hotel Randwyck for 1 day in the first week and 2 days in the second week Table 1
An experimental day starts at 0800hr and ends at 1900hr Figure 1 The different study days have exactly the same set-up with the cigarette brand smoked during the day being the only difference In this study it is important that the participants are able to smoke cigarettes ad libitum Because it is impossible to monitor this smoking topography at home The smoking topography is measured at a research location Therefore this study takes place in an apartment at Hotel Randwyck in a homelike atmosphere where standardized meals are served and cigarettes can be smoked when and how the participant desires The smoking topography of every smoked cigarette will be monitored through the CRESSmicro device which records the puff length the puff interval the puff flow and the puff volume Furthermore the exact time point of smoking ie the moment the cigarette is lit is noted in the experiment time table
Due to this setup the smoking topography measurements do not take place at scheduled time points and therefore only 2 participants per day will be measured Total duration of the study will be 3 months including 18 smoking individuals Table 1
Participants are their own controls by measuring baseline samples tbaseline This sampling takes place upon arrival before the first cigarette is smoked Consequently participants are asked not to smoke when waking up but to wait upon their arrival at the research location These baseline samplings include the collection of urine exhaled air blood a mouth swap and saliva
Goal of a study day is to follow the smoker in his personal daily life smoking schedule They can smoke when they want or feel the urge to smoke Therefore the sampling time points and the amount of cigarettes smoked are unknown per participant Figure 1 Despite the unknown time points on forehand smoking topography of every single cigarette within their presence at the research apartment is measured During the whole experiment experimental time is used The start of the experiment expected to be around 0900hr is the start of the experimental day noted as timepoint 0 This is probably shortly after the baseline measurements
All spent cigarette butts are collected in separate plastic tubes per participant with the experimental smoking time noted Because it is very important not to interfere with the daily life smoking schedule the experiment day is divided into timeslots for urine and a fixed time point for saliva sampling
Urine will be collected at baseline and during 2 time periods The first time point includes the baseline measurement before the first cigarette is smoked Next all urine between t0 and t5 hours is collected in 1 beaker and urine between t5 and t10 is collected in another beaker The participant is asked to empty his bladder just before the timeslots end This results in 3 urine samples per participant
Saliva and a mouth swap are collected at tbaseline t0 immediately after the first cigarette t5 and t10 This results in 4 saliva samples and mouth swaps per participant
The exhaled air and blood samples are collected before and immediately after smoking a cigarette to have the most accurate measure associated with the cigarette smoking However the smoking time points are uncertain due to the chosen setup of this study However since all smokers included smoke around 20 cigarettes a day they will at least smoke every 2 to 25 hours Therefore time periods of 25 hours in which the first cigarette smoked is used for sampling blood and exhaled air immediately after finishing smoking
At baseline the participant is asked to exhale via the nosemouth cap of the Owlstone a breathing device whereby the exhaled air passes the adsorption tubes The exhaled air just before and after finishing smoking the first cigarette is collected t0 The same is done for cigarettes smoked between t25 and t5 between t5 and t75 between t75 and t10 and the last sampling at T10 This results in 7 exhaled air samples per participant
To avoid multiple punctures the participants get a peripheral venous canula at baseline after which the baseline blood sample is withdrawn The next blood sample is withdrawn just before and after finishing smoking the first cigarette t0 Then the sampling points are just before and immediately after the first cigarette between t0 and t25 between t25 and t5 between t5 and t75 between t75 and t10 and the last sampling is at T10 For analysis of blood aldehydes an extra blood sample will be drawn from the canula at baseline and immediately after the first cigarette as well as immediately before and after the first cigarette after t5 and t75 This results in 13 blood sampling points per participant
In the future regulation of these harmful cigarette constituents should be based on more chemical classes as the WHO suggested However in order to introduce such class-based regulation a scientific base is needed to define upper limits of allowed amounts of chemicals groups in cigarette smoke emissions and to ensure decreased harmful effects due to cigarette smoking To date the causality between human exposure to specific cigarette smoke compounds and the harmful effects is unknown The first step in closing the gap in knowledge between cigarette smoke exposure and developing tobacco-related diseases includes a proper determination of human exposure to cigarette smoke chemicals
Unfortunately there is a lack of methodology to determine cigarette smoke exposure in humans
In a prospective observational pilot study in February 2016 natural human smoking behavior was characterized Smoking Topography Study 2016 NL5567606815 The smoking topography of every smoked Marlboro cigarette was monitored through the CRESSmicro device which records the puff length the puff interval the puff flow and the puff volume In this study the investigators could model the individual smoking profiles per participant by using data of 4 random cigarettes During modelling puff volume duration and thus also flow and interpuff interval were taken into account
Since there were no differences in smoking topography of cigarettes smoked observed at different times of the day implies that in this new study the researchers can invite participants for a shorter time because only data of 4 cigarettes is needed to adequately model their overall smoking profile needed for exposure measurements These smoking topography parameters are needed for the settings of a smoking machine applied to mimic the exact exposure to cigarette smoke toxicants for each individual
In our previous study the researchers concluded that smokers have their own individual smoking topography Of course smoking is also accompanied by the inhalation of harmful chemicals but the smoker is not aware of this during smoking and therefore does not adapt his smoking topography with respect to that exposure However it is unknown by what means the individual smoking topography parameters are related to toxicant exposure in the different parts of the lungs For example higher puff volumes with a different composition of toxicants lead to a different inhalation pattern than small puff volumes possible followed by different exposure in the lungs
In this new study the researchers want to include respiratory parameters of breathing and inhalation over the day as well Until now inhalation risk assessment is based on human breathing patterns while breathing and inhaling smoke are not the same due to the presence of harsh compounds in the smoke The current study aims to measure respiratory parameters to identify which lung compartments are exposed to what extent in relation to the observed smoking topography This can be achieved by respiratory inductive plethysmography RIP a non-invasive device that can be worn throughout the day In the current study the Hexoskin will be applied for these measurements
Literature describes that the process of smoking topography and inhalation differs per cigarette and situation In other words the smoker doses himself to gain nicotine with additional production of carbon monoxide CO and other harmful cigarette smoke-associated chemicals
As described before in the future cigarettes will be changed as they are only allowed to contain a maximum of certain toxicants One of the options to make cigarettes less addictive is to add less nicotine It is known that smokers show compensating behavior when smoking other cigarettes than their used to to gain the same amount of nicotine Our machine smoking experiments show that when smoking low-TNCO cigarettes the composition of the smoke per mg nicotine changes under the same smoking conditions This means that the cigarette characteristics are also at least partly responsible for the smoke composition The researchers are interested in how smoking behavior and inhalation and thus exposure changes when offering a smoker another type of cigarette
Therefore the researchers are interested whether the smoking behavior changes when the smoker has to smoke a low TNCO cigarette ie the Marlboro Prime The low values of nicotine in the smoke of the Marlboro Prime are partly achieved by ventilation holes in the cigarette filter By closing these filter vents with tape around the filter the smoke is no longer diluted with side stream air and the cigarette design is slightly different
The participants will smoke their normal brand Marlboro day 1 After the experimental day they receive the Marlboro Prime for smoking at home so they get used to smoking a new cigarette A week later the experimental day day 2 is repeated with this cigarette The participants can stay overnight or come back the next day day 3 to smoke the Prime cigarette while the ventilation holes of this low-TNCO cigarette are taped See the scheme in Table 1
As in the STS2016 this study again is aimed at measuring smoking in a habitual rhythm without imposing it as has been done thus far With this the natural smoking topography per cigarette can be measured in combination with the respiratory parameters by wearing a non-invasive RIP device By providing the participants with the Marlboro Prime cigarettes prior to the experimental days 2 and 3 they can get used to smoking them at home in order to develop natural smoking behavior
As during the last study the sampling is following a sampling scheme Figure 1 In summary during the day blood exhaled air urine and saliva will be sampled to measure nicotine carbon monoxide but also other cigarette smoke compounds such as aldehydes and their metabolites
In the future the personal smoking regimes of the participants and thus their personal exposures will be mimicked with machine smoking experiments This will be linked to the RIP parameters
Points of attention that came out of the STS 2016 include that smokers smoked less cigarettes than expected Reason for that is as they mentioned that they cannot smoke in the apartment while they smoke inside at home Another reason was that they have to smoke alone while at home or work there often is a co-smoker The participants didnt experience difficulties or changes in smoking behavior due to the use of CRESS They all practiced smoking with the CRESS the evening before the experimental day In the present study 2 smokers will be invited at the same time Again smokers have to go outside for a smoke to protect the researchers to secondhand smoke exposure This is kept as attainable as possible
Primary Objective
Is there a difference in natural smoking topography between Marlboro Marlboro Prime and Marlboro Prime taped leading to a different exposure for the smoker
Secondary Objectives
Is it possible to map personal smoking profiles with only 4 random cigarettes as showed in the previous Smoking Topography Study 2016
Is a different smoking topography profile per smoker related to deviating exposure of mainstream smoke in the respiratory tract compartments for the different cigarettes
Can exhaled air of smokers be used as biomarker for exposure to VOCsaldehydes
Are nicotine and toxicant metabolite levels in bloodurine changing over the day and connected to the smoking behavior of the different cigarettes
Study Design The Smoking Topography Study 2018 is a not-randomized cross-over study with a single group with the duration of 3 months Participants will visit our research apartment at Apart Hotel Randwyck for 1 day in the first week and 2 days in the second week Table 1
An experimental day starts at 0800hr and ends at 1900hr Figure 1 The different study days have exactly the same set-up with the cigarette brand smoked during the day being the only difference In this study it is important that the participants are able to smoke cigarettes ad libitum Because it is impossible to monitor this smoking topography at home The smoking topography is measured at a research location Therefore this study takes place in an apartment at Hotel Randwyck in a homelike atmosphere where standardized meals are served and cigarettes can be smoked when and how the participant desires The smoking topography of every smoked cigarette will be monitored through the CRESSmicro device which records the puff length the puff interval the puff flow and the puff volume Furthermore the exact time point of smoking ie the moment the cigarette is lit is noted in the experiment time table
Due to this setup the smoking topography measurements do not take place at scheduled time points and therefore only 2 participants per day will be measured Total duration of the study will be 3 months including 18 smoking individuals Table 1
Participants are their own controls by measuring baseline samples tbaseline This sampling takes place upon arrival before the first cigarette is smoked Consequently participants are asked not to smoke when waking up but to wait upon their arrival at the research location These baseline samplings include the collection of urine exhaled air blood a mouth swap and saliva
Goal of a study day is to follow the smoker in his personal daily life smoking schedule They can smoke when they want or feel the urge to smoke Therefore the sampling time points and the amount of cigarettes smoked are unknown per participant Figure 1 Despite the unknown time points on forehand smoking topography of every single cigarette within their presence at the research apartment is measured During the whole experiment experimental time is used The start of the experiment expected to be around 0900hr is the start of the experimental day noted as timepoint 0 This is probably shortly after the baseline measurements
All spent cigarette butts are collected in separate plastic tubes per participant with the experimental smoking time noted Because it is very important not to interfere with the daily life smoking schedule the experiment day is divided into timeslots for urine and a fixed time point for saliva sampling
Urine will be collected at baseline and during 2 time periods The first time point includes the baseline measurement before the first cigarette is smoked Next all urine between t0 and t5 hours is collected in 1 beaker and urine between t5 and t10 is collected in another beaker The participant is asked to empty his bladder just before the timeslots end This results in 3 urine samples per participant
Saliva and a mouth swap are collected at tbaseline t0 immediately after the first cigarette t5 and t10 This results in 4 saliva samples and mouth swaps per participant
The exhaled air and blood samples are collected before and immediately after smoking a cigarette to have the most accurate measure associated with the cigarette smoking However the smoking time points are uncertain due to the chosen setup of this study However since all smokers included smoke around 20 cigarettes a day they will at least smoke every 2 to 25 hours Therefore time periods of 25 hours in which the first cigarette smoked is used for sampling blood and exhaled air immediately after finishing smoking
At baseline the participant is asked to exhale via the nosemouth cap of the Owlstone a breathing device whereby the exhaled air passes the adsorption tubes The exhaled air just before and after finishing smoking the first cigarette is collected t0 The same is done for cigarettes smoked between t25 and t5 between t5 and t75 between t75 and t10 and the last sampling at T10 This results in 7 exhaled air samples per participant
To avoid multiple punctures the participants get a peripheral venous canula at baseline after which the baseline blood sample is withdrawn The next blood sample is withdrawn just before and after finishing smoking the first cigarette t0 Then the sampling points are just before and immediately after the first cigarette between t0 and t25 between t25 and t5 between t5 and t75 between t75 and t10 and the last sampling is at T10 For analysis of blood aldehydes an extra blood sample will be drawn from the canula at baseline and immediately after the first cigarette as well as immediately before and after the first cigarette after t5 and t75 This results in 13 blood sampling points per participant
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None