Ignite Creation Date:
2024-05-06 @ 11:19 AM
Last Modification Date:
2024-10-26 @ 12:43 PM
Study NCT ID:
NCT03490448
Status:
COMPLETED
Last Update Posted:
2018-04-06
First Post:
2018-03-22
Brief Title:
The Changes of Body Composition Glucolipid Metabolism and Bone Metabolism in Obese Children After Weight Loss
Sponsor:
Xinhua Hospital Shanghai Jiao Tong University School of Medicine
Organization:
Xinhua Hospital Shanghai Jiao Tong University School of Medicine
Study Overview
Official Title:
The Changes of Body Composition Glucolipid Metabolism and Bone Metabolism in Obese Children and Adolescents After Weight Loss Camp
Status:
COMPLETED
Status Verified Date:
2018-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Vitamin D plays a significant role in calcium and phosphorus homeostasis for maintaining structural integrity and function of musculoskeletal system Furthermore recent studies have revealed that vitamin D can decrease the risk of many conditions other than skeletal disease including autoimmune diseases cancers obesity and obesity-related diseases such as type 2 diabetes and cardiovascular disease Vitamin D may influence calcium absorption to affect obesity indirectly regulate adipocyte differentiation and relieve the development of metabolic syndrome by mediating levels of inflammatory factors
Another indicator of bone metabolism-osteocalcin may also be involved in energy metabolism and glucose metabolism and undercarboxylated osteocalcin ucOC is the form which has physiological activity ucOC may recombine with the receptors on the surface of pancreas β cells adipocytes hepatocytes and intestinal endocrine cell to regulate insulin secretion and insulin sensitivity
Currently the prevalence of vitamin D deficiency is a global problem in all age groups currently even in countries with sun exposure all year around The obesity group tend to have a higher incidence of vitamin D deficiencyMoreover the obesity group tend to have a higher incidence of vitamin D deficiency and a lower level of serum osteocalcin
This study observed the changes of body composition and glucolipid metabolism and bone metabolism during weight loss and investigated the correlations among them
Another indicator of bone metabolism-osteocalcin may also be involved in energy metabolism and glucose metabolism and undercarboxylated osteocalcin ucOC is the form which has physiological activity ucOC may recombine with the receptors on the surface of pancreas β cells adipocytes hepatocytes and intestinal endocrine cell to regulate insulin secretion and insulin sensitivity
Currently the prevalence of vitamin D deficiency is a global problem in all age groups currently even in countries with sun exposure all year around The obesity group tend to have a higher incidence of vitamin D deficiencyMoreover the obesity group tend to have a higher incidence of vitamin D deficiency and a lower level of serum osteocalcin
This study observed the changes of body composition and glucolipid metabolism and bone metabolism during weight loss and investigated the correlations among them
Detailed Description:
Obese children have a higher incidence of vitamin D deficiency VDD which resulted from unhealthy life style such as less time outdoors more sedentary time imbalance of dietary intake Adipose tissue is the storage position of vitamin D and the storage formation include 25-OHD2 and 25-OHD3 Theoretically the reserves of vitamin D in adipose tissue of obese children might release to the circulation after weight loss For further it is necessary to clarify the relationship between the improvement of metabolic risk with vitamin D status after weight loss
Osteocalcin is produced and secreted by osteoblast specifically Recent studies have shown that it regulated glucose metabolism and energy metabolism Obese group may have a lower level of serum osteocalcin Both 25-OHD and osteocalcin have association with energy metabolism This study will provide evidence to realize the relationship between bone metabolism and obesity
In our study all subjects were recruited from the obese children and adolescents aged 917 years who participated in six-week weight loss camp in July August 2014 Body mass index BMI was calculated as weight kg divided by height squared m2 Obesity was defined as having a BMI greater than or equal to the 95th percentile for age and sex according to WHO standard Exclusion criteria included 1 obesity caused by endocrine or heredity diseases eg hypothyroidism Prader-Willi syndrome single-gene defects 2 any disease influencing vitamin D metabolism eg such as metabolic bone diseases rickets nephritic syndrome and hepatic failure 3 any supplementation use or any medication affecting vitamin D metabolism use
All subjects underwent a closed-off weight loss program for six weeks The intervention methods included aerobic exercise and appropriate caloric control The dietary was designed on the basis of ensuring the daily energy physiological requirement and basal metabolic rate BMR was calculated to formulate diet project according to Harris-Benedict formula The diet was composed of 20 protein 30 fat and 50 carbohydrates During the camp all subjects had never taken any kinds of nutritional supplements
Before intervention all subjects received exercise load test to ensure safe and effective physical exercise In the exercise heart rate was monitored to ensure the small-medial load aerobic exercise The exercise programs included ball games such as badminton table tennis and basketball and also included jogging brisk walking swimming and cycle ergometer All kinds of sports were conducted indoor twice per day 6 days per week and lasted for 2 hours every time The weight loss camp was staffed by professional sports coaches and medical workers Exercise intensity was estimated by a formula exercise intensity target heart rate resting heart rate heart rate reserve maximum heart rate-resting heart rate 2040
Before and after intervention fasting blood samples were collected and sent to Shanghai Adicon Central Lab Test Menu immediately stored in 4C ice packs The indicators of glucolipid metabolism and bone metabolism were tested Total cholesterol TC with cholesterol oxidase triglyceride TG with enzyme method GPO-POD high density lipoprotein HDL and low density lipoprotein LDL with homogeneous methods fasting blood glucose FBG with hexokinase HK method fasting insulin FINS with chemiluminescence method Among the indicators of bone metabolism osteocalcin parathyroid hormone PTH and 25-OHD were assayed by electrochemiluminescence immunoassay while bone specific alkaline phosphatase BALP total propeptide of type I procollagen T-PINP and β-isomerized form carboxy-terminal telopeptide of type I collagen β-CTX were determined by immunoenzymatic methods
After blood samples were collected anthropometric parameters were measured including height Seca 264 Germany weight Biospace 370 South Korea triceps skinfold thickness TST and subscapular skinfold thickness SST skinfold caliper 689900 For error reduction every anthropometric measurement was conducted by the same trained personnels before and after weight-loss
Osteocalcin is produced and secreted by osteoblast specifically Recent studies have shown that it regulated glucose metabolism and energy metabolism Obese group may have a lower level of serum osteocalcin Both 25-OHD and osteocalcin have association with energy metabolism This study will provide evidence to realize the relationship between bone metabolism and obesity
In our study all subjects were recruited from the obese children and adolescents aged 917 years who participated in six-week weight loss camp in July August 2014 Body mass index BMI was calculated as weight kg divided by height squared m2 Obesity was defined as having a BMI greater than or equal to the 95th percentile for age and sex according to WHO standard Exclusion criteria included 1 obesity caused by endocrine or heredity diseases eg hypothyroidism Prader-Willi syndrome single-gene defects 2 any disease influencing vitamin D metabolism eg such as metabolic bone diseases rickets nephritic syndrome and hepatic failure 3 any supplementation use or any medication affecting vitamin D metabolism use
All subjects underwent a closed-off weight loss program for six weeks The intervention methods included aerobic exercise and appropriate caloric control The dietary was designed on the basis of ensuring the daily energy physiological requirement and basal metabolic rate BMR was calculated to formulate diet project according to Harris-Benedict formula The diet was composed of 20 protein 30 fat and 50 carbohydrates During the camp all subjects had never taken any kinds of nutritional supplements
Before intervention all subjects received exercise load test to ensure safe and effective physical exercise In the exercise heart rate was monitored to ensure the small-medial load aerobic exercise The exercise programs included ball games such as badminton table tennis and basketball and also included jogging brisk walking swimming and cycle ergometer All kinds of sports were conducted indoor twice per day 6 days per week and lasted for 2 hours every time The weight loss camp was staffed by professional sports coaches and medical workers Exercise intensity was estimated by a formula exercise intensity target heart rate resting heart rate heart rate reserve maximum heart rate-resting heart rate 2040
Before and after intervention fasting blood samples were collected and sent to Shanghai Adicon Central Lab Test Menu immediately stored in 4C ice packs The indicators of glucolipid metabolism and bone metabolism were tested Total cholesterol TC with cholesterol oxidase triglyceride TG with enzyme method GPO-POD high density lipoprotein HDL and low density lipoprotein LDL with homogeneous methods fasting blood glucose FBG with hexokinase HK method fasting insulin FINS with chemiluminescence method Among the indicators of bone metabolism osteocalcin parathyroid hormone PTH and 25-OHD were assayed by electrochemiluminescence immunoassay while bone specific alkaline phosphatase BALP total propeptide of type I procollagen T-PINP and β-isomerized form carboxy-terminal telopeptide of type I collagen β-CTX were determined by immunoenzymatic methods
After blood samples were collected anthropometric parameters were measured including height Seca 264 Germany weight Biospace 370 South Korea triceps skinfold thickness TST and subscapular skinfold thickness SST skinfold caliper 689900 For error reduction every anthropometric measurement was conducted by the same trained personnels before and after weight-loss
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None