Ignite Creation Date:
2024-05-06 @ 11:06 AM
Last Modification Date:
2024-10-26 @ 12:40 PM
Study NCT ID:
NCT03431532
Status:
COMPLETED
Last Update Posted:
2022-05-11
First Post:
2018-01-30
Brief Title:
Monocyte Subsets Altered by Anesthesia
Sponsor:
University Hospital Saarland
Organization:
Universität des Saarlandes
Study Overview
Official Title:
Neuraxial Versus General Anesthesia for Total Knee Replacement Surgery - Are There Different Effects on the Immune System
Status:
COMPLETED
Status Verified Date:
2018-02
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The impact of different anesthetic techniques on the immune system remains unclear
Aim of this ex vivo in vitro study was to determine the effects of general and neuraxial anesthesia on monocyte subset alteration and the release of prototypical pro- and anti-inflammatory cytokines Twenty patients undergoing total knee replacement surgery were randomly assigned to receive either general anesthesia ITN or combined spinalepidural anesthesia CSE CD14 and HLA-DR expression patterns on monocytes and intracellular TNF-alpha production were quantified via flow cytometry TNF-α and IL-10 release were measured via enzyme linked immunosorbent assay ELISA
Aim of this ex vivo in vitro study was to determine the effects of general and neuraxial anesthesia on monocyte subset alteration and the release of prototypical pro- and anti-inflammatory cytokines Twenty patients undergoing total knee replacement surgery were randomly assigned to receive either general anesthesia ITN or combined spinalepidural anesthesia CSE CD14 and HLA-DR expression patterns on monocytes and intracellular TNF-alpha production were quantified via flow cytometry TNF-α and IL-10 release were measured via enzyme linked immunosorbent assay ELISA
Detailed Description:
Numerous of factors affect the immunological response during surgery Despite intensive research the impact of different anesthetic techniques on the immune system remains unclear
Aim of this ex vivo in vitro study was to determine the effects of general and neuraxial anesthesia on monocyte subset alteration and the release of prototypical pro- and anti-inflammatory cytokines Twenty patients undergoing total knee replacement surgery were randomly assigned to receive either general anesthesia ITN or combined spinalepidural anesthesia CSE Samples of venous blood were taken from the patients before and after induction of anesthesia immediately 6 hours 24 hours and 48 hours after surgery All blood samples were incubated in presence or absence of LPS lipopolysaccharide 1 µgml for 24 hours CD14 and HLA-DR expression patterns on monocytes and intracellular TNF-alpha production were quantified via flow cytometry TNF-α and IL-10 release were measured via enzyme linked immunosorbent assay ELISA
Aim of this ex vivo in vitro study was to determine the effects of general and neuraxial anesthesia on monocyte subset alteration and the release of prototypical pro- and anti-inflammatory cytokines Twenty patients undergoing total knee replacement surgery were randomly assigned to receive either general anesthesia ITN or combined spinalepidural anesthesia CSE Samples of venous blood were taken from the patients before and after induction of anesthesia immediately 6 hours 24 hours and 48 hours after surgery All blood samples were incubated in presence or absence of LPS lipopolysaccharide 1 µgml for 24 hours CD14 and HLA-DR expression patterns on monocytes and intracellular TNF-alpha production were quantified via flow cytometry TNF-α and IL-10 release were measured via enzyme linked immunosorbent assay ELISA
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None