Ignite Creation Date:
2024-05-05 @ 4:41 PM
Last Modification Date:
2024-10-26 @ 9:23 AM
Study NCT ID:
NCT00294177
Status:
UNKNOWN
Last Update Posted:
2006-02-20
First Post:
2006-02-17
Brief Title:
Conformation of Beta Human Chorionic Gonadotropin During Chemotherapy for Choriocarcinoma
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
Conformation of Beta Human Chorionic Gonadotropin During Chemotherapy for Persistent Gestational Trophoblastic Tumors Using Atomic Force Microscopy and Dual Polarisation Interferometry
Status:
UNKNOWN
Status Verified Date:
2006-02
Last Known Status:
NOT_YET_RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Gestational trophoblastic disease comprises a spectrum of diseases with different propensity for local invasion and metastasis that is partial and complete hydatidiform mole choriocarcinoma and placental site trophoblastic tumorAll trophoblastic tumors produce hCG and monitoring of therapy is largely based on the determination of hCG in serum The conformational change of tumor markers during antineoplastic chemotherapy for cancer and its clinical meaning has only been rarely studied In this study we will try to understand the conformational change of the tumor marker hCG during chemotherapy
Detailed Description:
The conformational change of human chorionic gonadotropin beta hCG beta during chemotherapy for gestational trophoblastic disease and its clinical meaning have not been well understood In this study we will use atomic force microscopy and dual polarisation interferometry to measure the dimensions and conformational change of beta human chorionic gonadotropin during chemotherapy for persistent gestational trophoblastic tumors
Gestational trophoblastic disease comprises a spectrum of diseases with different propensity for local invasion and metastasis that is partial and complete hydatidiform mole choriocarcinoma and placental site trophoblastic tumor Persistent trophoblastic disease may develop both from partial and complete moles All trophoblastic tumors produce human chorionic gonadotropin hCG and monitoring of therapy is largely based on the determination of hCG in serum The more malignant forms also express excessive amounts of hCG beta and simultaneous determination of hCG and hCG beta can be used to differentiate between molar disease and trophoblastic cancer which is associated with a proportion of hCG beta exceeding 5 Over glycosylated hCG has also been reported to be a characteristic of trophoblastic cancer but its clinical utility remains to be established The hCG beta level in serum is also used to evaluate prognosis with not only very high but also very low levels in relation to tumor burden being indicative of adverse prognosis Because of that 1 more malignant forms also express excessive amounts of hCG beta 2 the half-life of hCG beta is longer than that of hCG 3 the hCG beta hCG ratio will increase when the levels decrease after successful therapy and 4 the ratio has been found to increase during development of therapy-resistant disease monitoring of gestational trophoblastic disease usually relies on human chorionic gonadotropin beta measurement Treatment of choriocarcinoma is most often monitored by serial assay of hCG beta in serum until the levels are undetectable and simultaneous determination of hCG beta can sometimes reveal a relapse earlier than hCG Therefore measurement of hCG beta is important in treatment of gestational trophoblastic tumors
Human choriogonadotropin belongs to a family of heterodimeric glycoprotein hormones which are comprised of two noncovalently bonded subunits with four N-linked carbohydrates During chemotherapy the hCG beta molecules can be degraded A study has been reported by crystallographic analysis by which they found that crystals of deglycosylated human chorionic gonadotropin has a hexagonal bipyramidal structure using a method of vapor diffusion against ammonium sulfate Previously conformation of the hCG molecule has rarely been studied By chemical method over glycosylated hCG has been reported to be a characteristic of trophoblastic cancer but its clinical utility remains to be established Photoelectric method is a newly developed method to study the conformation of the protein or glycoprotein molecules As yet there is no report about the conformation of hCG serial studies Gestational trophoblastic tumor as well as other gynecological cancers often underwent antitumor chemotherapy and monitor by tumor markers During the antineoplastic chemotherapies the conformational change of tumor markers including hCG beta in persistent gestational trophoblastic tumor for cancer and its clinical meaning has only been rarely studied Up to the present time it is still not well understood whether a conformational change or reduced stability of the heterodimer may occur during chemotherapy
In this study we will use an atomic force microscopy AFM to probe the surface nanostructure of beta human chorionic gonadotropin The conformational change of the beta human chorionic gonadotropin which is secreted by persistent gestational trophoblastic tumors during chemotherapy will be studied We will also want to directly visualize a single molecular structure of beta human chorionic gonadotropin and quantitative measurements of the dimensions of the glycoprotein will be provided The average height calculated for each hCG beta particle will be measured and compared subsequently during chemotherapy Moreover a experiment using dual polarization interferometric DPI as a biosensor will also be performed and the average monolayer thickness value will also be calculated To investigate further the surface ultrastructure of a hCG beta molecule a hCG beta sample at very low concentration will be scanned in vacuum by AFM The higher-resolution images will clearly reveal the shape of hCG beta molecules In addition phase images of hCG beta molecules will be captured simultaneously with their height images and the lateral dimensions of the shape of hCG beta molecules will then be measured and calculated The average values calculated for the outside diameter and pore diameter will thus be obtained This study will represent the first direct characterization of the surface ultrastructure of the hCG beta molecule at a nanometer scale The physical measurements of the outer diameter pore diameter and protomer diameter in the hCG beta by AFM and DPI may suggest how to recognize hCG beta molecules which constitute an important tumor marker during chemotherapy for persistent gestational trophoblastic tumors Unraveling the molecular structure and measuring the dimensions of hCG beta may provide new directions for future application in clinical hCG beta sensor chips In can also be likely that the dimensional change of hCG beta molecules may also have some clinical implications for persistent gestational trophoblastic tumors
Gestational trophoblastic disease comprises a spectrum of diseases with different propensity for local invasion and metastasis that is partial and complete hydatidiform mole choriocarcinoma and placental site trophoblastic tumor Persistent trophoblastic disease may develop both from partial and complete moles All trophoblastic tumors produce human chorionic gonadotropin hCG and monitoring of therapy is largely based on the determination of hCG in serum The more malignant forms also express excessive amounts of hCG beta and simultaneous determination of hCG and hCG beta can be used to differentiate between molar disease and trophoblastic cancer which is associated with a proportion of hCG beta exceeding 5 Over glycosylated hCG has also been reported to be a characteristic of trophoblastic cancer but its clinical utility remains to be established The hCG beta level in serum is also used to evaluate prognosis with not only very high but also very low levels in relation to tumor burden being indicative of adverse prognosis Because of that 1 more malignant forms also express excessive amounts of hCG beta 2 the half-life of hCG beta is longer than that of hCG 3 the hCG beta hCG ratio will increase when the levels decrease after successful therapy and 4 the ratio has been found to increase during development of therapy-resistant disease monitoring of gestational trophoblastic disease usually relies on human chorionic gonadotropin beta measurement Treatment of choriocarcinoma is most often monitored by serial assay of hCG beta in serum until the levels are undetectable and simultaneous determination of hCG beta can sometimes reveal a relapse earlier than hCG Therefore measurement of hCG beta is important in treatment of gestational trophoblastic tumors
Human choriogonadotropin belongs to a family of heterodimeric glycoprotein hormones which are comprised of two noncovalently bonded subunits with four N-linked carbohydrates During chemotherapy the hCG beta molecules can be degraded A study has been reported by crystallographic analysis by which they found that crystals of deglycosylated human chorionic gonadotropin has a hexagonal bipyramidal structure using a method of vapor diffusion against ammonium sulfate Previously conformation of the hCG molecule has rarely been studied By chemical method over glycosylated hCG has been reported to be a characteristic of trophoblastic cancer but its clinical utility remains to be established Photoelectric method is a newly developed method to study the conformation of the protein or glycoprotein molecules As yet there is no report about the conformation of hCG serial studies Gestational trophoblastic tumor as well as other gynecological cancers often underwent antitumor chemotherapy and monitor by tumor markers During the antineoplastic chemotherapies the conformational change of tumor markers including hCG beta in persistent gestational trophoblastic tumor for cancer and its clinical meaning has only been rarely studied Up to the present time it is still not well understood whether a conformational change or reduced stability of the heterodimer may occur during chemotherapy
In this study we will use an atomic force microscopy AFM to probe the surface nanostructure of beta human chorionic gonadotropin The conformational change of the beta human chorionic gonadotropin which is secreted by persistent gestational trophoblastic tumors during chemotherapy will be studied We will also want to directly visualize a single molecular structure of beta human chorionic gonadotropin and quantitative measurements of the dimensions of the glycoprotein will be provided The average height calculated for each hCG beta particle will be measured and compared subsequently during chemotherapy Moreover a experiment using dual polarization interferometric DPI as a biosensor will also be performed and the average monolayer thickness value will also be calculated To investigate further the surface ultrastructure of a hCG beta molecule a hCG beta sample at very low concentration will be scanned in vacuum by AFM The higher-resolution images will clearly reveal the shape of hCG beta molecules In addition phase images of hCG beta molecules will be captured simultaneously with their height images and the lateral dimensions of the shape of hCG beta molecules will then be measured and calculated The average values calculated for the outside diameter and pore diameter will thus be obtained This study will represent the first direct characterization of the surface ultrastructure of the hCG beta molecule at a nanometer scale The physical measurements of the outer diameter pore diameter and protomer diameter in the hCG beta by AFM and DPI may suggest how to recognize hCG beta molecules which constitute an important tumor marker during chemotherapy for persistent gestational trophoblastic tumors Unraveling the molecular structure and measuring the dimensions of hCG beta may provide new directions for future application in clinical hCG beta sensor chips In can also be likely that the dimensional change of hCG beta molecules may also have some clinical implications for persistent gestational trophoblastic tumors
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| Taiwan NSC 94WFA0101857 | None | None | None |