Ignite Creation Date:
2024-05-05 @ 4:37 PM
Last Modification Date:
2024-10-26 @ 9:22 AM
Study NCT ID:
NCT00275886
Status:
UNKNOWN
Last Update Posted:
2006-01-12
First Post:
2006-01-11
Brief Title:
Activation and Recruitment of GIL During Hp Infection
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
Activation and Recruitment of Gastric Infiltrating Lymphocytes During H Pylori Infection
Status:
UNKNOWN
Status Verified Date:
2005-12
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Helicobacter pylori is a major human pathogen that infects over half of the world population Infection initiates a series of changes in the gastric mucosa beginning with gastritis and leading in some patients to peptic ulcer disease mucosa-associated lymphomas and gastric adenocarcinoma It is believed that host factors in particular the T cell-mediated immune responses may play an important role in the pathogenesis of diseases induced by H pylori infection Recent results revealed that there were higher IFN-γ secreting cells in gastric infiltrating T cells isolated from H pylori infected patients than in uninfected patients suggesting that the TH1 response and degree of IFN-γ production is associated with disease severity Meanwhile recent studies have shown that apoptosis of the gastric epithelium is increased during infection and this response is associated with an expansion of gastric T-helper type 1 Th1 cells In this project we are trying to further investigate role of host T cell mediated immune response in pathogenesis of Helicobacter infection by characterization of the expression of chemokine receptors on gastric infiltrating lymphocytes We are going to investigate the mechanisms involving in chemokinechemokine receptor interaction in recruitment of gastric infiltrating lymphocytes and pathogenesis of gastric mucosa damage in Helicobacter infection This study will be helpful for understanding the mechanisms of activation and recruitment of gastric-infiltrating lymphocytes during gastric inflammation
Detailed Description:
Specific Aim 1 Characterization of the gastric infiltrating T cells from H pylori gastritis patients A Patients H pylori infected gastritis and asymptomatic patients are selected from National Taiwan University hospital The gastric biopsy specimen are taken from endoscopic biopsy in routine health check up or panendoscopic examination for the despeptic patients HP negative gastritis patients will also be studied for negative control
B Immunohistochemical identification of T cells and expression of chemokine receptors
Specific Aim 2 Isolation and characterization of chemokines and chemokine receptors of the infiltrating lymphocytes in H pylori-associated gastritis tissue
A Isolation of gastric infiltrating lymphocyte B Identification of the expression of chemokine receptor in gastric infiltrating lymphocytes by flow cytometry C Detection of expression of chemokines in gastric tissues
B Immunohistochemical identification of T cells and expression of chemokine receptors
Specific Aim 2 Isolation and characterization of chemokines and chemokine receptors of the infiltrating lymphocytes in H pylori-associated gastritis tissue
A Isolation of gastric infiltrating lymphocyte B Identification of the expression of chemokine receptor in gastric infiltrating lymphocytes by flow cytometry C Detection of expression of chemokines in gastric tissues
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None