Ignite Creation Date:
2024-05-06 @ 10:15 AM
Last Modification Date:
2024-10-26 @ 12:27 PM
Study NCT ID:
NCT03208777
Status:
UNKNOWN
Last Update Posted:
2017-07-07
First Post:
2017-07-03
Brief Title:
Telomeric Abnormalities in Colorectal Diseases by Fluorescent in Situ Hybridization Technique
Sponsor:
Assiut University
Organization:
Assiut University
Study Overview
Official Title:
Telomeric Abnormalities in Benign and Malignant Colorectal Diseases by Fluorescent in Situ Hybridization Technique
Status:
UNKNOWN
Status Verified Date:
2017-07
Last Known Status:
NOT_YET_RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Colorectal carcinoma is a heterogeneous disease that is caused by the interaction of genetic and environmental factors colorectal carcinoma encompasses a complex disease with different molecular pathways and biological characteristics arising from a multi-step process that implicates several genetic and epigenetic events The multi-step genetic model involves the loss of function of tumor suppressor genes such as adenomatous polyposis coli APC Telomeres could be a promising marker due to the fact that their lengths change in the colorectal polyp-carcinoma sequence Moreover telomere length TL is altered in blood cells in patients with colorectal carcinoma
These findings could suggest that changes in TL may take place before the development of the tumor
The two main forms of inflammatory bowel disease IBD ulcerative colitis UC and Crohns disease CD are characterized by chronic intestinal inflammation and risk of progression to colon cancer One proposed cause of the latter characteristic is chromosome instability since the rearrangement of genetic material can lead to activation of oncogenes loss of tumor suppressor genes and other changes that lead to uncontrolled cell growth Chromosome instability is particularly associated with UC and has been observed in colon epithelial cells and peripheral blood mononuclear cell Since genomic instability in peripheral blood mononuclear cells PBMCs has been used as a biomarker for global cancer risk in a number of diseases the latter observation suggests the possibility of a chromosome instability syndrome in UC that could affect all tissues One possible cause of chromosome instability is telomere dysfunction
These findings could suggest that changes in TL may take place before the development of the tumor
The two main forms of inflammatory bowel disease IBD ulcerative colitis UC and Crohns disease CD are characterized by chronic intestinal inflammation and risk of progression to colon cancer One proposed cause of the latter characteristic is chromosome instability since the rearrangement of genetic material can lead to activation of oncogenes loss of tumor suppressor genes and other changes that lead to uncontrolled cell growth Chromosome instability is particularly associated with UC and has been observed in colon epithelial cells and peripheral blood mononuclear cell Since genomic instability in peripheral blood mononuclear cells PBMCs has been used as a biomarker for global cancer risk in a number of diseases the latter observation suggests the possibility of a chromosome instability syndrome in UC that could affect all tissues One possible cause of chromosome instability is telomere dysfunction
Detailed Description:
Human chromosomes are capped and stabilized by telomeres which not only protect them from damage but also have a role in regulating cellular senescence After reaching a critical length telomeres experience a double DNA change and cells will eventually enter senescence replication or cell death Telomere length and telomere shortening have been long hypothesized to be a biological marker of aging at the cellular level and a potential mechanism of carcinogenesis Genomic instability is a critical factor in the initiation and progression of human cancers One mechanism that underlies genomic instability is loss of telomere function
fluorescent in situ hybridization is a molecular diagnostic technique that utilizes labeled DNA probes to detect or confirm gene or chromosome abnormalities fluorescent in situ hybridization is often utilized for both research and diagnosis of hematological malignancies and solid tumors Conceptually fluorescent in situ hybridization is a very straightforward technique whereby a DNA probe is hybridized to its complementary sequence on chromosomal preparations previously fixed on microscope slides fluorescent in situ hybridization is able to detect cells that have chromosomal abnormalities consistent with neoplasia
There has been a surge of published studies which assessed the association between telomere length and development of colorectal carcinoma Thus a meta-analysis addressing colorectal carcinoma and telomere length would be a useful addition to the current information in this area
fluorescent in situ hybridization is a molecular diagnostic technique that utilizes labeled DNA probes to detect or confirm gene or chromosome abnormalities fluorescent in situ hybridization is often utilized for both research and diagnosis of hematological malignancies and solid tumors Conceptually fluorescent in situ hybridization is a very straightforward technique whereby a DNA probe is hybridized to its complementary sequence on chromosomal preparations previously fixed on microscope slides fluorescent in situ hybridization is able to detect cells that have chromosomal abnormalities consistent with neoplasia
There has been a surge of published studies which assessed the association between telomere length and development of colorectal carcinoma Thus a meta-analysis addressing colorectal carcinoma and telomere length would be a useful addition to the current information in this area
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None