Ignite Creation Date:
2024-05-05 @ 12:07 PM
Last Modification Date:
2024-10-26 @ 9:20 AM
Study NCT ID:
NCT00243776
Status:
RECRUITING
Last Update Posted:
2024-06-05
First Post:
2005-10-24
Brief Title:
Molecular and Cellular Characterization of Cardiac Tissue in Postnatal Development
Sponsor:
Emory University
Organization:
Emory University
Study Overview
Official Title:
Molecular and Cellular Characterization of Cardiac Tissue in Postnatal Development
Status:
RECRUITING
Status Verified Date:
2024-06
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The study team will use small pieces of human hearts which are removed as part of a required surgical procedure to study different objectives One of the objective is how calcium ions pass through the membrane of heart cells in order to tell the heart cell how much force to contract with when the heart beats Investigators will also study the proteins and RNA of these pieces to determine how the newborn heart cells control their force of contraction differently from adult heart cells Investigators hypothesize that infant hearts have different regulation of calcium entry than adult hearts The study team also wants to study combinations of 3D cardiac spheres with multiple environmental cues that can improve functional and metabolic maturation of Human pluripotent stem cell-derived cardiomyocytes hPSC-CMs and generate a more clinically relevant cell model
Detailed Description:
Extrapolating pharmacological and surgical therapies from adult AD studies to infant INF patients is problematic because the knowledge of cellular electrophysiology and molecular biology of human INF heart cells is limited The investigators have studied developmental differences in rabbit ventricular cells and now extend these studies to atrial and ventricular cells isolated from AD young adult YAD or INF patients
The study aims are as follows
1 Developmental differences in transient outward current of atrial cells Investigators will extend their studies to isolated cells and tissue from YADs age 14-20 In addition several other accessory beta-subunits have been found in cardiac myocytes and may interact with Kv channels and regulate the function of these channels The study team will determine relative amounts of these putative regulators of human atrial Ito to determine which correlate with developmental changes in Ito kinetics
2 Developmental differences in amplitude and regulation of calcium current in atrial cells Investigators hypothesize that INF atrial cells have tonic inhibition of adenylyl cyclase and thus of ICa mediated by inhibitory G proteins possibly related to constitutive activity of the adenosine A1 receptor and that compared to AD or YAD cells have greater sensitivity to inhibitors of phosphatases and phosphodiesterases and that developmental changes in basal ICa amplitude and beta-sympathetic modulation correlate with inhibitory G protein levels receptor numbers for M2 and A1 receptors and constitutive inhibitory activity
3 Modulation of atrial cell calcium transients by changes in AP waveform and developmental age The study team will test the hypothesis that prolongation of the early repolarization phase of the after potential AP increases Ca2 entry and that YAD cells have faster removal of Ca2 from cytoplasm than INF cells and will determine if the Na- Ca2 exchange current INCX is greater in INF vs AD or YAD cells
4 Developmental differences in Ca current and transients and contractility in ventricular cells Investigators propose that INF cells and tissue have lower basal ICa lower potency for Isoproterenol stimulation higher levels of Gialpha3 and A1 receptors greater inhibitory potency for adenosine and tonic inhibition of ICa We also propose that the YAD cells have lower levels of NCX and lower INCX higher levels of SERCA and faster removal of Ca2 from the cytoplasm Previous animal studies have indicated various developmental changes in cardiac cells We will specifically study human postnatal developmental changes in Ito regulation of ICa and intracellular Ca2 transients
5 Structural Functional and Metabolic Maturation of hPSC-CMs Investigators propose that combinations of 3D cardiac spheres with multiple environmental cues to improve mitochondrial fatty acid oxidation FAO or beta-oxidation pathway will promote functional and metabolic maturation of hPSC-CMs and generate a more clinically relevant model using tissue engineering combined with pharmacological agents to regulate signals that are involved in FAO metabolism and appropriate growth factor and hormonal signaling that mimic the microenvironment for the maturation of CMs
The study aims are as follows
1 Developmental differences in transient outward current of atrial cells Investigators will extend their studies to isolated cells and tissue from YADs age 14-20 In addition several other accessory beta-subunits have been found in cardiac myocytes and may interact with Kv channels and regulate the function of these channels The study team will determine relative amounts of these putative regulators of human atrial Ito to determine which correlate with developmental changes in Ito kinetics
2 Developmental differences in amplitude and regulation of calcium current in atrial cells Investigators hypothesize that INF atrial cells have tonic inhibition of adenylyl cyclase and thus of ICa mediated by inhibitory G proteins possibly related to constitutive activity of the adenosine A1 receptor and that compared to AD or YAD cells have greater sensitivity to inhibitors of phosphatases and phosphodiesterases and that developmental changes in basal ICa amplitude and beta-sympathetic modulation correlate with inhibitory G protein levels receptor numbers for M2 and A1 receptors and constitutive inhibitory activity
3 Modulation of atrial cell calcium transients by changes in AP waveform and developmental age The study team will test the hypothesis that prolongation of the early repolarization phase of the after potential AP increases Ca2 entry and that YAD cells have faster removal of Ca2 from cytoplasm than INF cells and will determine if the Na- Ca2 exchange current INCX is greater in INF vs AD or YAD cells
4 Developmental differences in Ca current and transients and contractility in ventricular cells Investigators propose that INF cells and tissue have lower basal ICa lower potency for Isoproterenol stimulation higher levels of Gialpha3 and A1 receptors greater inhibitory potency for adenosine and tonic inhibition of ICa We also propose that the YAD cells have lower levels of NCX and lower INCX higher levels of SERCA and faster removal of Ca2 from the cytoplasm Previous animal studies have indicated various developmental changes in cardiac cells We will specifically study human postnatal developmental changes in Ito regulation of ICa and intracellular Ca2 transients
5 Structural Functional and Metabolic Maturation of hPSC-CMs Investigators propose that combinations of 3D cardiac spheres with multiple environmental cues to improve mitochondrial fatty acid oxidation FAO or beta-oxidation pathway will promote functional and metabolic maturation of hPSC-CMs and generate a more clinically relevant model using tissue engineering combined with pharmacological agents to regulate signals that are involved in FAO metabolism and appropriate growth factor and hormonal signaling that mimic the microenvironment for the maturation of CMs
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| R01HL077485 | NIH | None | None |
| 35328 | OTHER_GRANT | American Heart Association | httpsreporternihgovquickSearchR01HL077485 |