Ignite Creation Date:
2024-05-06 @ 9:18 AM
Last Modification Date:
2024-10-26 @ 12:13 PM
Study NCT ID:
NCT02959723
Status:
UNKNOWN
Last Update Posted:
2016-11-09
First Post:
2015-04-23
Brief Title:
Physiopathology of Pulmonary Arterial Hypertension Mechanistic Studies
Sponsor:
Universitaire Ziekenhuizen KU Leuven
Organization:
Universitaire Ziekenhuizen KU Leuven
Study Overview
Official Title:
Physiopathology of Pulmonary Arterial Hypertension Mechanistic Studies
Status:
UNKNOWN
Status Verified Date:
2016-11
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The current aims to combine analysis of different inflammatory biomarkers and BMPR2 mutations which are currently analyzed in each patient diagnosed with idiopathic or familial PAH to establish an earlier diagnosis and consequently better orientate the therapeutic strategy in PAH
Detailed Description:
BACKGROUND
Pulmonary hypertension is a severe disorder defined by an elevated mean pulmonary arterial pressure mPAP as 25 mmHg at rest A classification of the different types of pulmonary hypertension has been established according to shared pathologic and clinical features as well as similar therapeutic options and recently updated Five major categories have been defined including 1 pulmonary arterial hypertension PAH 2 PH owing to left heart diseases 3 PH owing to lung diseases andor hypoxia 4 chronic thromboembolic PH CTEPH and 5 PH with unclear multifactorial mechanisms
PAH commonly defined by an elevated PAP 25 mmHg at rest and a normal pulmonary arterial wedge pressure 15 mmHg is characterized by a pre-capillary arteriopathy with the presence of vascular remodeling and formation of plexiform lesions an increased pulmonary vascular resistance PVR which may result in right heart failure PAH may be idiopathic IPAH heritable HPAH or associated with drugtoxin exposure or other medical conditions including connective tissue diseases congenital heart diseases human immunodeficiency virus or portal hypertension Familial cases were already reported in the early fifties and in 2000 bone morphogenic protein receptor type 2 gene BMPR2 was identified as the gene responsible for more than 70 of HPAH and about 20 of IPAH BMPR2 belongs to a superfamily of growth factor receptors including bone morphogenic protein BMPs and transforming growth factor beta TGF-β and consequently controls cellular functions such as proliferation migration differentiation or apoptosis BMPR2 mutations may favor activation of p38MAPK-dependent pro-proliferative pathways which is also a key player in cytokine-induced inflammatory signaling pathways Although BMPR2 mutation carriers develop PAH 10 years earlier than non-carriers and display more severe hemodynamic changes only 20 of BMPR2 mutation carriers will further develop PAH A role of inflammation in the pathogenesis of PAH has been suggested A predictive role of various cytokines has been recently evidenced in PAH The investigators have observed elevated C-reactive protein CRP circulating levels in PAH and evidenced a predictive role of CRP in PAH Interestingly heterozygous null BMPR2 mice failed to develop PAH unless an additional inflammatory insult was applied More recently inhibition of leukocyte recruitment has been shown to impair PAH progression in mice with genetic ablation of endothelial BMPR-II This consequently suggests that inflammation could play the role of a second hit in BMPR2 mutation carriers to further develop PAH
Besides lipoprotein-associated phospholipase A2 Lp-PLA2 also named plasma platelet-activating factor-acetylhydrolase PAF-AH is the product of PLA2G7 gene and is an enzyme capable of inactivating platelet-activating factor PAF a potent lipid mediator of inflammation and a potent vasoconstrictor and its analogs Several studies have evidenced an increase in PAF-AH mass and activity in hypercholesterolemia and coronary heart disease In a recent meta-analysis including more than 70000 patients from 32 epidemiologic studies high circulating PAF-AH levels have been identified as an independent predictive risk factor for cardiovascular events When over-expressed in rodents Lp-PLA2 displayed anti-inflammatory properties and reduced atherogenesis suggesting a potential anti-inflammatory role of Lp-PLA2 Lp-PLA2 polymorphisms have been identified and Lp-PLA2 activity has been strongly associated with genetic variants related to LDL- cholesterol levels In a preliminary study the investigators have observed a diminished Lp-PLA2 activity in the plasma of PAH patients together with low total and LDL-cholesterol levels
HYPOTHESIS OBJECTIVES
Regarding previous and current results by the investigators they hypothesized that combining analysis of different inflammatory biomarkers and BMPR2 mutations which are currently analyzed in each patient diagnosed with idiopathic or familial PAH could contribute to establish an earlier diagnosis and consequently better orientate the therapeutic strategy Moreover BMPR2 gene mutations are responsible for more than 70 of HPAH and about 20 of IPAH among human BMPR2 mutation carriers only 20 will further develop PAH suggesting that BMPR2 mutation itself is not the unique cause for developing PAH and that a second hit such as inflammation would be required and play a triggering role in the development of PAH
METHODOLOGY
1 Identification of biomarkers
For the current study one aim is to identify one or several biomarkers which could predict adverse outcome in PAH To address this aim the investigators would like to evaluate the levels of CRP total cholesterol HDL-cholesterol triglycerides albumin Lp-PLA2 activity Thrombin Activatable Fibrinolysis Inhibitor TAFI and vitamin D in blood plasma from all patients diagnosed with PAH at the UZ Leuven Campus Gasthuisberg
To perform these analyses the investigators would need 5 tubes of blood
one on EDTA to be sent to the Hospital Laboratory CRP lipid profile and albumin
one on citrate to further measure TAFI Laboratory of Pharmaceutical Biology KU Leuven
one on EDTA to prepare plasma to be further stored in the laboratory of Pneumology KU Leuven from which an aliquot will be sent to INSERM UMRS937 Paris France to measure Lp-PLA2 activity
one on EDTA is sent to the CME genomic DNA isolation to study BMPR2 ALK-1 and ENG mutations genomic DNA will be stored for potential future genetic
one with Silica Clot Activator to be sent to the Hospital Laboratory vitamin D
2 Human pulmonary arterial cell isolation
Another aim is to investigate whether a relationship between impaired BMPR2 signaling and inflammation could result in dysfunction of pulmonary arterial cells in PAH To achieve our objective the investigators propose to investigate
i the in vitro effects of inflammatory mediators and macrophages on the activation of human pre-capillary pulmonary arterial endothelial cell PAEC and on the proliferating and migrating properties of PASMC from BMPR2 carriers and non-carriers PAH patients
ii expression and distribution of inflammatory mediators their receptors and BMPR2 in human lung parenchyma and distal pulmonary arteries
iii the ex-vivo vasoreactive effects of inflammatory mediators mentioned above on isolated human pre-capillary pulmonary arteries
iv Moreover pulmonary arterial endothelial cells will be harvested from patients undergoing right heart catheterization using a recently described technique consisting in isolating endothelial cells from the balloon of a Swan-Ganz catheter commonly used to performed right heart catheterization in patients
3 Pulmonary tissue collection
Lung parenchyma will be collected from i PAH patients at the time of lung transplantation ii eventually unused donor lungs and iii tissue surrounding lung tumour resection from patients without COPD Pulmonary pre-capillary endothelial and smooth muscle cells will be isolated maintained in culture and further stored at low passage number in liquid nitrogen
Two pieces of lung parenchyma will be snap frozen for further RNA isolation and protein extraction one piece will be fixed in paraformaldehyde and further processed for immunohistochemistry analyses
Pulmonary hypertension is a severe disorder defined by an elevated mean pulmonary arterial pressure mPAP as 25 mmHg at rest A classification of the different types of pulmonary hypertension has been established according to shared pathologic and clinical features as well as similar therapeutic options and recently updated Five major categories have been defined including 1 pulmonary arterial hypertension PAH 2 PH owing to left heart diseases 3 PH owing to lung diseases andor hypoxia 4 chronic thromboembolic PH CTEPH and 5 PH with unclear multifactorial mechanisms
PAH commonly defined by an elevated PAP 25 mmHg at rest and a normal pulmonary arterial wedge pressure 15 mmHg is characterized by a pre-capillary arteriopathy with the presence of vascular remodeling and formation of plexiform lesions an increased pulmonary vascular resistance PVR which may result in right heart failure PAH may be idiopathic IPAH heritable HPAH or associated with drugtoxin exposure or other medical conditions including connective tissue diseases congenital heart diseases human immunodeficiency virus or portal hypertension Familial cases were already reported in the early fifties and in 2000 bone morphogenic protein receptor type 2 gene BMPR2 was identified as the gene responsible for more than 70 of HPAH and about 20 of IPAH BMPR2 belongs to a superfamily of growth factor receptors including bone morphogenic protein BMPs and transforming growth factor beta TGF-β and consequently controls cellular functions such as proliferation migration differentiation or apoptosis BMPR2 mutations may favor activation of p38MAPK-dependent pro-proliferative pathways which is also a key player in cytokine-induced inflammatory signaling pathways Although BMPR2 mutation carriers develop PAH 10 years earlier than non-carriers and display more severe hemodynamic changes only 20 of BMPR2 mutation carriers will further develop PAH A role of inflammation in the pathogenesis of PAH has been suggested A predictive role of various cytokines has been recently evidenced in PAH The investigators have observed elevated C-reactive protein CRP circulating levels in PAH and evidenced a predictive role of CRP in PAH Interestingly heterozygous null BMPR2 mice failed to develop PAH unless an additional inflammatory insult was applied More recently inhibition of leukocyte recruitment has been shown to impair PAH progression in mice with genetic ablation of endothelial BMPR-II This consequently suggests that inflammation could play the role of a second hit in BMPR2 mutation carriers to further develop PAH
Besides lipoprotein-associated phospholipase A2 Lp-PLA2 also named plasma platelet-activating factor-acetylhydrolase PAF-AH is the product of PLA2G7 gene and is an enzyme capable of inactivating platelet-activating factor PAF a potent lipid mediator of inflammation and a potent vasoconstrictor and its analogs Several studies have evidenced an increase in PAF-AH mass and activity in hypercholesterolemia and coronary heart disease In a recent meta-analysis including more than 70000 patients from 32 epidemiologic studies high circulating PAF-AH levels have been identified as an independent predictive risk factor for cardiovascular events When over-expressed in rodents Lp-PLA2 displayed anti-inflammatory properties and reduced atherogenesis suggesting a potential anti-inflammatory role of Lp-PLA2 Lp-PLA2 polymorphisms have been identified and Lp-PLA2 activity has been strongly associated with genetic variants related to LDL- cholesterol levels In a preliminary study the investigators have observed a diminished Lp-PLA2 activity in the plasma of PAH patients together with low total and LDL-cholesterol levels
HYPOTHESIS OBJECTIVES
Regarding previous and current results by the investigators they hypothesized that combining analysis of different inflammatory biomarkers and BMPR2 mutations which are currently analyzed in each patient diagnosed with idiopathic or familial PAH could contribute to establish an earlier diagnosis and consequently better orientate the therapeutic strategy Moreover BMPR2 gene mutations are responsible for more than 70 of HPAH and about 20 of IPAH among human BMPR2 mutation carriers only 20 will further develop PAH suggesting that BMPR2 mutation itself is not the unique cause for developing PAH and that a second hit such as inflammation would be required and play a triggering role in the development of PAH
METHODOLOGY
1 Identification of biomarkers
For the current study one aim is to identify one or several biomarkers which could predict adverse outcome in PAH To address this aim the investigators would like to evaluate the levels of CRP total cholesterol HDL-cholesterol triglycerides albumin Lp-PLA2 activity Thrombin Activatable Fibrinolysis Inhibitor TAFI and vitamin D in blood plasma from all patients diagnosed with PAH at the UZ Leuven Campus Gasthuisberg
To perform these analyses the investigators would need 5 tubes of blood
one on EDTA to be sent to the Hospital Laboratory CRP lipid profile and albumin
one on citrate to further measure TAFI Laboratory of Pharmaceutical Biology KU Leuven
one on EDTA to prepare plasma to be further stored in the laboratory of Pneumology KU Leuven from which an aliquot will be sent to INSERM UMRS937 Paris France to measure Lp-PLA2 activity
one on EDTA is sent to the CME genomic DNA isolation to study BMPR2 ALK-1 and ENG mutations genomic DNA will be stored for potential future genetic
one with Silica Clot Activator to be sent to the Hospital Laboratory vitamin D
2 Human pulmonary arterial cell isolation
Another aim is to investigate whether a relationship between impaired BMPR2 signaling and inflammation could result in dysfunction of pulmonary arterial cells in PAH To achieve our objective the investigators propose to investigate
i the in vitro effects of inflammatory mediators and macrophages on the activation of human pre-capillary pulmonary arterial endothelial cell PAEC and on the proliferating and migrating properties of PASMC from BMPR2 carriers and non-carriers PAH patients
ii expression and distribution of inflammatory mediators their receptors and BMPR2 in human lung parenchyma and distal pulmonary arteries
iii the ex-vivo vasoreactive effects of inflammatory mediators mentioned above on isolated human pre-capillary pulmonary arteries
iv Moreover pulmonary arterial endothelial cells will be harvested from patients undergoing right heart catheterization using a recently described technique consisting in isolating endothelial cells from the balloon of a Swan-Ganz catheter commonly used to performed right heart catheterization in patients
3 Pulmonary tissue collection
Lung parenchyma will be collected from i PAH patients at the time of lung transplantation ii eventually unused donor lungs and iii tissue surrounding lung tumour resection from patients without COPD Pulmonary pre-capillary endothelial and smooth muscle cells will be isolated maintained in culture and further stored at low passage number in liquid nitrogen
Two pieces of lung parenchyma will be snap frozen for further RNA isolation and protein extraction one piece will be fixed in paraformaldehyde and further processed for immunohistochemistry analyses
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| S57114 | OTHER | UZ Leuven | None |