Ignite Creation Date:
2024-05-06 @ 9:12 AM
Last Modification Date:
2024-10-26 @ 12:11 PM
Study NCT ID:
NCT02928367
Status:
UNKNOWN
Last Update Posted:
2020-01-09
First Post:
2016-10-06
Brief Title:
The Effect of Leukocyte Dna mEthylation and micRoBIOME Diversity on Host Defense Mechanisms During Community-acquired Pneumonia ELDER-BIOME
Sponsor:
Academisch Medisch Centrum - Universiteit van Amsterdam AMC-UvA
Organization:
Academisch Medisch Centrum - Universiteit van Amsterdam AMC-UvA
Study Overview
Official Title:
The Effect of Leukocyte Dna mEthylation and micRoBIOME Diversity on Host Defense Mechanisms During Community-acquired Pneumonia
Status:
UNKNOWN
Status Verified Date:
2020-01
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
ELDER-BIOME
Brief Summary:
Community-acquired pneumonia CAP represents a major health care problem and mortality and morbidity associated with severe pneumonia remain considerable despite state of the art care
While the role of altered DNA methylation in cancer has been widely studied knowledge of its impact on antibacterial defense is highly limited In addition recent preclinical studies showed that the gut and respiratory microbiota contributes to host defense against bacterial pneumonia
This study aims to explore a completely novel research area linking the extent of DNA methylation in blood leukocyte monocytes and neutrophils and function of gut and respiratory microbiota on the influence of innate immune responses to and host defense against CAP
While the role of altered DNA methylation in cancer has been widely studied knowledge of its impact on antibacterial defense is highly limited In addition recent preclinical studies showed that the gut and respiratory microbiota contributes to host defense against bacterial pneumonia
This study aims to explore a completely novel research area linking the extent of DNA methylation in blood leukocyte monocytes and neutrophils and function of gut and respiratory microbiota on the influence of innate immune responses to and host defense against CAP
Detailed Description:
Rationale
Community-acquired pneumonia CAP represents a major health care problem and mortality and morbidity associated with severe pneumonia remain considerable despite state of the art care
While the role of altered DNA methylation in cancer has been widely studied knowledge of its impact on antibacterial defense is highly limited In addition recent preclinical studies showed that the gut and respiratory microbiota contributes to host defense against bacterial pneumonia
This study aims to explore a completely novel research area linking the extent of DNA methylation in blood leukocyte monocytes and neutrophils and function of gut and respiratory microbiota on the influence of innate immune responses to and host defense against CAP
Primary Objectives
1 To obtain insight in the role of altered DNA methylation in blood leukocytes monocytes and neutrophils in innate immune responses and host defense in patients with CAP
2 To determine the composition and function of the gut and respiratory microbiota in patients with CAP
Secondary Objective
1 To assess the influence of the gut microbiota on leukocyte DNA methylation in patients with CAP
Study design
Observational study among patients with CAP at the Emergency Department and Internal Medicine Ward of the Academic Medical Center Amsterdam
Study population
231 CAP patients and 115 healthy subjects above 18 years of age
Methods
From above mentioned patients and healthy volunteers a maximum of 90 ml of blood will be drawn to analyze DNA methylation patterns of purified monocytes and neutrophils which will be analyzed in connection with DNA methyltransferase and ten eleven translocation TET activity RNA gene expression and a selection of standard innate immune function tests Moreover isolated monocytes and neutrophils from admission samples will be stimulated in vitro with Streptococcus pneumoniae and Klebsiella pneumoniae In addition rectal and nasopharyngeal swabs will be obtained to investigate the role of the gut and respiratory microbiota composition and function Patient material will be obtained upon inclusion and on day 28 when patients will be seen in the outpatient clinic for follow up
Nature and extent of the burden and risks associated with participation benefit and group relatedness
Participating in this observational study will not benefit the participants and healthy volunteers The study will provide information about the influence of leukocyte DNA methylation as well as the gut and respiratory microbiota on host defense mechanisms during CAP The knowledge obtained can potentially benefit CAP patients in the future by providing alternative immune modulating treatment options that modify the host response The burden and risks for patients participating in the ELDER-BIOME study is minimal The investigators will take 90ml of blood 4 rectal swabs and 2 nasopharyngeal swabs divided over two time-points day of presentation and day 28 post presentation Healthy volunteers will be subjected to one blood draw 70 ml and 2 rectal - and 2 nasopharyngeal swabs at one time-point
Community-acquired pneumonia CAP represents a major health care problem and mortality and morbidity associated with severe pneumonia remain considerable despite state of the art care
While the role of altered DNA methylation in cancer has been widely studied knowledge of its impact on antibacterial defense is highly limited In addition recent preclinical studies showed that the gut and respiratory microbiota contributes to host defense against bacterial pneumonia
This study aims to explore a completely novel research area linking the extent of DNA methylation in blood leukocyte monocytes and neutrophils and function of gut and respiratory microbiota on the influence of innate immune responses to and host defense against CAP
Primary Objectives
1 To obtain insight in the role of altered DNA methylation in blood leukocytes monocytes and neutrophils in innate immune responses and host defense in patients with CAP
2 To determine the composition and function of the gut and respiratory microbiota in patients with CAP
Secondary Objective
1 To assess the influence of the gut microbiota on leukocyte DNA methylation in patients with CAP
Study design
Observational study among patients with CAP at the Emergency Department and Internal Medicine Ward of the Academic Medical Center Amsterdam
Study population
231 CAP patients and 115 healthy subjects above 18 years of age
Methods
From above mentioned patients and healthy volunteers a maximum of 90 ml of blood will be drawn to analyze DNA methylation patterns of purified monocytes and neutrophils which will be analyzed in connection with DNA methyltransferase and ten eleven translocation TET activity RNA gene expression and a selection of standard innate immune function tests Moreover isolated monocytes and neutrophils from admission samples will be stimulated in vitro with Streptococcus pneumoniae and Klebsiella pneumoniae In addition rectal and nasopharyngeal swabs will be obtained to investigate the role of the gut and respiratory microbiota composition and function Patient material will be obtained upon inclusion and on day 28 when patients will be seen in the outpatient clinic for follow up
Nature and extent of the burden and risks associated with participation benefit and group relatedness
Participating in this observational study will not benefit the participants and healthy volunteers The study will provide information about the influence of leukocyte DNA methylation as well as the gut and respiratory microbiota on host defense mechanisms during CAP The knowledge obtained can potentially benefit CAP patients in the future by providing alternative immune modulating treatment options that modify the host response The burden and risks for patients participating in the ELDER-BIOME study is minimal The investigators will take 90ml of blood 4 rectal swabs and 2 nasopharyngeal swabs divided over two time-points day of presentation and day 28 post presentation Healthy volunteers will be subjected to one blood draw 70 ml and 2 rectal - and 2 nasopharyngeal swabs at one time-point
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None