Ignite Creation Date:
2024-05-06 @ 9:01 AM
Last Modification Date:
2024-10-26 @ 12:09 PM
Study NCT ID:
NCT02886013
Status:
COMPLETED
Last Update Posted:
2016-09-01
First Post:
2016-08-21
Brief Title:
FTO rs9939609 and PPARy rs1801282 Polymorphisms in Mexican Adolescents
Sponsor:
Asociación Científica Latina AC
Organization:
Asociación Científica Latina AC
Study Overview
Official Title:
FTO rs9939609 and PPARy rs1801282 Polymorphisms in Mexican Adolescents With Overweight and Obesity at High Risk for Developing Diabetes
Status:
COMPLETED
Status Verified Date:
2016-08
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Background and Aims The presence of the FTO rs9939609 and PPARy rs1801282 polymorphisms suggests changes in energy metabolism this variation may be responsible for the development of various diseases including obesity The aim of this study was to identify the presence of these polymorphisms in Mexican adolescents with overweight and obesity at high risk for developing diabetes Methods and Results This was a descriptive cross-sectional study where 71 healthy adolescents average age of 16 were included Anthropometric measurements Body mass index as well as the determination of glucose insulin and HOMA index were calculated from all the patients The FTO rs9939609 and PPARy rs1801282 polymorphisms were determined by real-time PCR
Detailed Description:
Study Population Students without known disease between 14 and 18 years from the Lic Adolfo Lopez Mateos Preparatory school of the Autonomous University of the State of Mexico UAEMex were invited to participate
Anthropometry The body mass index BMI was calculated as weight kg divided by height m squared with conventional stadiometer dividing the results in normal overweight and obesity according to the classification of the World Health Organization WHO specifically for adolescents by gender and age
Laboratory 3 mL of venous blood Vacutainer tubes were taken in fasting The concentration of glucose total cholesterol HDL-cholesterol LDL-cholesterol and triglycerides were determined by enzymatic methods Randox Laboratories Ltd County Antrim UK whereas leptin and adiponectin concentrations were determined by the immunosorbent assay ELISA Invitrogen MR Frederyck MD USA A sample remained frozen at -70C 900 series Thermo Scientific until DNA extraction
Genotyping DNA extraction was performed in the Magna Pure LC 20 Instrument Roche Germany using the MagNA Pure LC DNA Isolation Kit 1 Roche Germany After extraction the DNA was quantified using a NanoPhotometer Implen GmbH Germany reporting concentration μgml and purity 260280 absorbance Genotyping was performed by polymerase chain reaction PCR Life express thermal cycler Bioer China The primers and conditions for each polymorphism are listed in Table 1
The oligonucleotides were designed using the Primer Quest web tool Integrated DNA Technologies Inc CA USA and synthesized at the Synthesis and DNA Sequencing Unit of the National Autonomous University of Mexico UNAM Institute of Biotechnology Cuernavaca Morelos Mexico A search was performed in BLAST to verify the correct hybridization The final products were visualized in 2 agarose gels stained with ethidium bromide and visualized under an UV transilluminator Gel Logic 212 Pro Carestream USA To confirm the detection of polymorphisms sequencing was performed at the National Institute of Genomic Medicine INMEGEN Mexico City
Anthropometry The body mass index BMI was calculated as weight kg divided by height m squared with conventional stadiometer dividing the results in normal overweight and obesity according to the classification of the World Health Organization WHO specifically for adolescents by gender and age
Laboratory 3 mL of venous blood Vacutainer tubes were taken in fasting The concentration of glucose total cholesterol HDL-cholesterol LDL-cholesterol and triglycerides were determined by enzymatic methods Randox Laboratories Ltd County Antrim UK whereas leptin and adiponectin concentrations were determined by the immunosorbent assay ELISA Invitrogen MR Frederyck MD USA A sample remained frozen at -70C 900 series Thermo Scientific until DNA extraction
Genotyping DNA extraction was performed in the Magna Pure LC 20 Instrument Roche Germany using the MagNA Pure LC DNA Isolation Kit 1 Roche Germany After extraction the DNA was quantified using a NanoPhotometer Implen GmbH Germany reporting concentration μgml and purity 260280 absorbance Genotyping was performed by polymerase chain reaction PCR Life express thermal cycler Bioer China The primers and conditions for each polymorphism are listed in Table 1
The oligonucleotides were designed using the Primer Quest web tool Integrated DNA Technologies Inc CA USA and synthesized at the Synthesis and DNA Sequencing Unit of the National Autonomous University of Mexico UNAM Institute of Biotechnology Cuernavaca Morelos Mexico A search was performed in BLAST to verify the correct hybridization The final products were visualized in 2 agarose gels stained with ethidium bromide and visualized under an UV transilluminator Gel Logic 212 Pro Carestream USA To confirm the detection of polymorphisms sequencing was performed at the National Institute of Genomic Medicine INMEGEN Mexico City
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None