Ignite Creation Date:
2024-05-06 @ 8:30 AM
Last Modification Date:
2024-10-26 @ 12:01 PM
Study NCT ID:
NCT02756923
Status:
COMPLETED
Last Update Posted:
2023-03-24
First Post:
2016-04-13
Brief Title:
Regulation of Stem Spermatogonia in the Mature Testis
Sponsor:
Weill Medical College of Cornell University
Organization:
Weill Medical College of Cornell University
Study Overview
Official Title:
Regulation of Stem Spermatogonia in the Mature Testis
Status:
COMPLETED
Status Verified Date:
2023-03
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Investigative trial to evaluate the role of a glial cell lined derived neurotrophic factor GDNF in regulation of spermatogonial renewal and testicular function Goal of the trial is to provide greater information on the mechanisms that effect stem spermatogonial maintenance renewal and proliferation in its relation to male infertility
Detailed Description:
An essential requirement for sustaining male fertility is maintaining an adequate number of stem spermatogonia the foundation of spermatogenesis To achieve this when the stem cells divide some progeny must remain stem spermatogonia while other progeny differentiate It is obvious that the correct balance between self-renewing replication and differentiation of stem spermatogonia is crucial to male fertility and there is a indirect evidence that GDNF plays an important role in maintaining this balance in the normal mature testis However almost nothing is known about the in vivo regulation of this balance in the mature organ of the specific function of GDNF in the adult testis or if physiological changes in GDNF expression significantly affect the replication or differentiation of the stem cells To address these critical issues a unique mouse model that allows GDNF signaling to the stem spermatogonia to be specifically and reversibly inhibited in vivo by an ATP antagonist With this model the first direct evidence that GDNF is required for maintaining the stem spermatogonial pool in a normal mature testis Additionally the investigators have shown that when inhibition of GDNF signaling is reversed the stem cells begin to rebuild the stem cell pool Importantly our data demonstrate that some stem spermatogonia are lost when GDNF signaling is inhibited for as little as 2 days while other stem cells survive for up to 11 days This suggests that factors intrinsic or extrinsic to the stem cells modulate the response to GDNF Using this new mouse model the mechanisms responsible for the loss of stem spermatogonia proliferation and regeneration will be investigated along with GDNF signaling and inhibition At the end of all of this these studies will be done on waste tissue obtained from normal men and men with infertility who otherwise have testicular surgery for therapeutic purposes
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 1P50HD096723-01A1 | NIH | None | httpsreporternihgovquickSearch1P50HD096723-01A1 |