Viewing Study NCT02430116



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Last Modification Date: 2024-10-26 @ 11:42 AM
Study NCT ID: NCT02430116
Status: UNKNOWN
Last Update Posted: 2015-04-30
First Post: 2015-04-20

Brief Title: Mitochondrial Apoptotic Pathway Induced by Myocardial Ischemia-Reperfusion Injury in Human
Sponsor: WANG Yan-bin
Organization: Shenzhen Sun Yat-sen Cardiovascular Hospital

Study Overview

Official Title: Mitochondrial Apoptotic Pathway Induced by Myocardial Ischemia-Reperfusion Injury in Human
Status: UNKNOWN
Status Verified Date: 2015-04
Last Known Status: RECRUITING
Delayed Posting: No
If Stopped, Why?: Not Stopped
Has Expanded Access: False
If Expanded Access, NCT#: N/A
Has Expanded Access, NCT# Status: N/A
Acronym: None
Brief Summary: Background The cardiomyocytes apoptosis induced by ischemia-reperfusionIR is one of the most important factors in the myocardial IR injuryMIRI undergoing cardiac valve replacement with cardiopulmonary bypassCVRCPBand Ischemic postconditioning I-postC can inhibit apoptosis of myocardial cells Consequently this study investigated the key genes and apoptosis signaling pathways of myocardium in patients undergoing CVRCPB

Methods A total of 36 New York Heart Association class II or III patients with rheumatic heart disease RHD of both sexes aged 21-59 years who were scheduled for first cardiac valve replacement with CPB in the investigators hospital from February 2014 to May 2015 were randomly divided into the following three groups n12 each negative control group NEG group IR group POS group and I-postC group Treat group In the Treat group the procedure involved 5 min before opening the ascending aorta aortic unclamping for 30 s and cross-clamping for 30 s for three cycles after which the ascending aorta was completely opened The NEG and Treat groups were not treated Thirty-six patients were assessed for arrhythmia and recovery of myocardial contractile function after reperfusion by electrocardiograms and degree of dependence on vasoactive drugs The myocardial tissues of the right atrial appendage were obtained at 3 min before CPB was established in the NEG group and at 45 min after opening the aorta in the POS and Treat groups In all three groups the myocardial tissues of the right atrial appendage were obtained and preserved at -80C for further experiments The right atrial appendage of three patients randomly selected in each group was fixed with RNA later Qiagen Hilden Germany in a centrifuge tube overnight at 4C and then preserved at -20C for RNA extraction Human 12135K Gene Array profiling of mRNA expressions was undertaken in human cardiac muscle cells Differentially expressed mRNAs verified by quantitative real-time RT-PCR were subjected to pathway analysis The mRNA expressions of AIF APAF1 CYCS Bax caspase-3 caspase-9 caspase-6 caspase-7 BCL2 BAG1 and PI3K were assessed by real-time RT-PCR and western blot analysis The levels of myocardial apoptosis induced by IR were investigated by TUNEL assays The changes in MIRI induced by myocardial apoptosis were investigated by pathologic examination of the myocardium
Detailed Description: None

Study Oversight

Has Oversight DMC: None
Is a FDA Regulated Drug?: None
Is a FDA Regulated Device?: None
Is an Unapproved Device?: None
Is a PPSD?: None
Is a US Export?: None
Is an FDA AA801 Violation?: None