Ignite Creation Date:
2024-05-06 @ 3:38 AM
Last Modification Date:
2024-10-26 @ 11:36 AM
Study NCT ID:
NCT02331966
Status:
UNKNOWN
Last Update Posted:
2015-01-06
First Post:
2015-01-05
Brief Title:
Molecular Pathways Involved in the Pathogenesis and Behavior of Mesenchymal Phosphaturic Tumors Causing Oncogenic Osteomalacia
Sponsor:
AHEPA University Hospital
Organization:
AHEPA University Hospital
Study Overview
Official Title:
Molecular Pathways Involved in the Pathogenesis and Behavior of Mesenchymal Phosphaturic Tumors
Status:
UNKNOWN
Status Verified Date:
2015-01
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The tumors that cause oncogenic osteomalacia TIO express and release in the circulation phosphaturic factors such as fibroblast growth factor-23 FGF-23 that decrease renal phosphate absorption through acting in the proximal renal tubule and decreasing Type 2a and 2c sodium-phosphate co-transporter They typically follow a benign clinical course and even in the rare malignant cases local recurrence occurs in less than 5 and distant metastasis are very uncommon
In this study we aim to investigate the role of other molecular pathways such as ERK1 ERK2 mTOR EGFR MEK1 MEK2 VEGFR3 AKT1 AKT2 IGFR-1 IGFR-2 PDGFRA PDGFRB cMET FGFR2 apart from FGF23 KLOTHO and PHEX in the behavior of histopathologically benign mesenchymal phosphaturic tumors
In this study we aim to investigate the role of other molecular pathways such as ERK1 ERK2 mTOR EGFR MEK1 MEK2 VEGFR3 AKT1 AKT2 IGFR-1 IGFR-2 PDGFRA PDGFRB cMET FGFR2 apart from FGF23 KLOTHO and PHEX in the behavior of histopathologically benign mesenchymal phosphaturic tumors
Detailed Description:
Study Protocol Cell Culture Bone marrow and tissue samples will be obtained from the patients after they will give their written informed consent Material will be maintained in RPMI culture medium Sigma R0883 Germany Peripheral blood mononuclear cells PBMCs from healthy donors will be used as control For detection of cancer cells in our samples we perform flow cytometry using EpCAM magnetic beads 39-EPC-50 Gentaur and the negative selection cells non-cancerous are isolated and then cultured in a 25-cm2 flask 5520100 Orange Scientific with RPMI-1640 medium R6504 Sigma
Molecular analysis RNA is extracted from cell cultures using RNeasy Mini Kit 74105 Qiagen Hilden Germany iScript cDNA synthesis kit 1708891 Bio-Rad CA USA is used for cDNA synthesis and Real-time polymerase chain reaction PCR is performed using the iTaq Universal SYBR Green Supermix 1725124 Bio-Rad Specific primers for each marker and for an endogenous control gene 18S rRNA is designed using Genamic Expression 11 software A universal Reference RNA consisting of 10 human cancer cell lines 740000-41 Agilent as well as human genomic DNA G304A Promega will be used in quantitative PCR qPCR reactions Statistical analysis The qPCR results will be tested according to the Kolmogorov-Smirnov test All the reactions molecular assays flow cytometry are performed in triplicates A p value 005 is considered significant
Molecular analysis RNA is extracted from cell cultures using RNeasy Mini Kit 74105 Qiagen Hilden Germany iScript cDNA synthesis kit 1708891 Bio-Rad CA USA is used for cDNA synthesis and Real-time polymerase chain reaction PCR is performed using the iTaq Universal SYBR Green Supermix 1725124 Bio-Rad Specific primers for each marker and for an endogenous control gene 18S rRNA is designed using Genamic Expression 11 software A universal Reference RNA consisting of 10 human cancer cell lines 740000-41 Agilent as well as human genomic DNA G304A Promega will be used in quantitative PCR qPCR reactions Statistical analysis The qPCR results will be tested according to the Kolmogorov-Smirnov test All the reactions molecular assays flow cytometry are performed in triplicates A p value 005 is considered significant
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None