Ignite Creation Date:
2024-05-05 @ 11:55 AM
Last Modification Date:
2024-10-26 @ 9:16 AM
Study NCT ID:
NCT00172939
Status:
UNKNOWN
Last Update Posted:
2005-09-15
First Post:
2005-09-12
Brief Title:
Molecular Mechanisms of Helium-Neon Laser on Melanocyte Regeneration in Skin Equivalent Vitiligo Model
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
Molecular Mechanisms of Helium-Neon Laser on Melanocyte Regeneration in Skin Equivalent Vitiligo Model
Status:
UNKNOWN
Status Verified Date:
2005-03
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
This current three-year proposal aims to clarify the mechanisms of melanocyte destruction and regeneration in vitiligo lesions using both traditional cell culture and skin equivalent model organotypic culture
Detailed Description:
Melanocytes MCs are melanin-producing cells of the skin that are derived from neural crest cells Vitiligo vulgaris is a common depigmentation disorder resulting from destruction of functional MCs in the affected skin Although this disorder affects all races and occurs in approximately 1 of the world population its pathogenesis remains obscure Recovery from vitiligo is initiated by the activation proliferation and migration of melanoblasts MBs to the epidermis The subsequent maturation of MBs leads to production of melanosomes that will be transferred to the juxtaposed keratinocytes The beam of a low-energy laser produces a temperature elevation of less than 05 Therefore light-mediated reaction by such laser irradiation is referred to as biostimulation Mitochondrial cytochrome c oxidase is considered as a photoacceptor of low-energy laser Low-energy He-Ne laser has numberous clinical applications Our previous studies showed that He-Ne laser irradiation can induce repigmentation in vitiligo vulgaris However the exact mechanisms of He-Ne laser irradiation in repigmentation are not elucidated thoroughly In the past we have demonstrated the coexistence of both antikertinocyte anti-KC and antimelanocyte anti-MC IgG antibodies Abs in vitiligo patients and explored their potential roles in vitiligo This current three-year proposal aims to clarify the mechanisms of melanocyte destruction and regeneration in vitiligo lesions using both traditional cell culture and skin equivalent model organotypic culture In the first year we shall focus on the regeneration of MCs and MBs by He-Ne laser with or without the presence of anti-MC IgG antibodies from vitiligo patients as well as the involved photodynamic mechanisms Our goal for the second year is to investigate the regeneration of MCs and MBs by He-Ne laser with or without the presence of anti-KC IgG antibodies from vitiligo patients In the final year of our project we shall explore the effects of He-Ne laser combine antibody-dependent cellular cytotoxicity ADCC on the regeneration of MCs and MBs Our results will provide more information for the effectiveness of He-Ne laser irradiation in treating vitiligo
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None