Ignite Creation Date:
2024-05-05 @ 11:54 AM
Last Modification Date:
2024-10-26 @ 9:15 AM
Study NCT ID:
NCT00162422
Status:
UNKNOWN
Last Update Posted:
2008-10-29
First Post:
2005-09-11
Brief Title:
Genetic Determinants of the Bronchodilatation Effect of Albuterol ex-Vivo
Sponsor:
Hadassah Medical Organization
Organization:
Hadassah Medical Organization
Study Overview
Official Title:
The Effect of β2 Genetic Polymorphisms on the Brochodilatation Effect of Albuterol ex-Vivo
Status:
UNKNOWN
Status Verified Date:
2008-10
Last Known Status:
RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The role played by ß2AR polymorphisms in determining the bronchial response to ß2AR agonist drugs has been confirmed by several studies
The purpose of the present study is to examine possible causal relationships between genetically based alteration in the structure of ß2AR and drug responsiveness An ex-vivo model organ bath technique will be used to investigate association between polymorphisms in the coding region of ß2AR and the response of bronchial rings derived from human lung tissue to the respective agonists ie salbutamol
In the second part of the study the same bronchial rings will be incubated for 24 hours in a solution containing fixed concentration of albuterol A second dose response curve for rising concentrations of albuterol will be constructed and a relationship between β2 genetic polymorphisms and the extent of desensitization to albuterol will be investigated
In addition through specifically designed receptor binding assay the bronchial tissue will be used to define the affinity Kd and expression Vmax of the ß2 receptor These data will enable to enhance our understanding regarding the mechanism responsible for the association noted between ß2AR polymorphism and altered drug effect
The purpose of the present study is to examine possible causal relationships between genetically based alteration in the structure of ß2AR and drug responsiveness An ex-vivo model organ bath technique will be used to investigate association between polymorphisms in the coding region of ß2AR and the response of bronchial rings derived from human lung tissue to the respective agonists ie salbutamol
In the second part of the study the same bronchial rings will be incubated for 24 hours in a solution containing fixed concentration of albuterol A second dose response curve for rising concentrations of albuterol will be constructed and a relationship between β2 genetic polymorphisms and the extent of desensitization to albuterol will be investigated
In addition through specifically designed receptor binding assay the bronchial tissue will be used to define the affinity Kd and expression Vmax of the ß2 receptor These data will enable to enhance our understanding regarding the mechanism responsible for the association noted between ß2AR polymorphism and altered drug effect
Detailed Description:
None
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None