Ignite Creation Date:
2024-05-05 @ 11:51 AM
Last Modification Date:
2024-10-26 @ 9:15 AM
Study NCT ID:
NCT00155493
Status:
UNKNOWN
Last Update Posted:
2005-09-12
First Post:
2005-09-09
Brief Title:
Age-Related Changes in Proliferation and Differentiation Capacity of Human Preadipocytes From Periorbital Fat
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
None
Status:
UNKNOWN
Status Verified Date:
2005-06
Last Known Status:
NOT_YET_RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Periorbital rejuvenation has been an issue of great interest in plastic surgery Adipose tissue plays an important role in the periorbital anatomy and has a great impact on the aging process of the region We believe age-related atrophy of the facial adipose tissue may be related to the decreased proliferation and differentiation capability of the preadipocytes adipose precursor cells
Detailed Description:
Understanding the age-related changes in periorbital adipose tissue is crucial for improving current rejuvenation techniques or developing new ones However no such study has been reported in the literature
Hundreds of people came to our hospital requesting upper blepharoplasty every year Taking advantage of the discarded soft tissue in the blepharoplasty surgery samples of bilateral pretarsal fat can be easily obtained from patients of various ages This study is designed to objectively evaluate the age-related changes of the preadipocytes in human periorbital adipose tissue regarding their proliferation and differentiation capability
The adipose samples taken during the surgery are sent to the lab immediately to separate the preadipocytes from the specimens Their number is estimated under the microscope and then the cells are cultured in vitro to evaluate their proliferation and differentiation capability Proliferation is assessed by counting the number of preadipocyte everyday for 7 days and differentiation is assessed by Glycerol-3-phosphate dehydrogenase GPDH assay
A linear regression analysis is used to analyze correlations of the parameters with age Moreover by dividing the subjects who had their periorbital fat sampled during upper blepharoplasty operation into three age groups the parameters are compared with ANOVA test Therefore the age-related changes of the proliferation and differentiation capability of the preadipocytes in human periorbital adipose tissue can be concluded
Hundreds of people came to our hospital requesting upper blepharoplasty every year Taking advantage of the discarded soft tissue in the blepharoplasty surgery samples of bilateral pretarsal fat can be easily obtained from patients of various ages This study is designed to objectively evaluate the age-related changes of the preadipocytes in human periorbital adipose tissue regarding their proliferation and differentiation capability
The adipose samples taken during the surgery are sent to the lab immediately to separate the preadipocytes from the specimens Their number is estimated under the microscope and then the cells are cultured in vitro to evaluate their proliferation and differentiation capability Proliferation is assessed by counting the number of preadipocyte everyday for 7 days and differentiation is assessed by Glycerol-3-phosphate dehydrogenase GPDH assay
A linear regression analysis is used to analyze correlations of the parameters with age Moreover by dividing the subjects who had their periorbital fat sampled during upper blepharoplasty operation into three age groups the parameters are compared with ANOVA test Therefore the age-related changes of the proliferation and differentiation capability of the preadipocytes in human periorbital adipose tissue can be concluded
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None