Ignite Creation Date:
2024-05-05 @ 11:51 AM
Last Modification Date:
2024-10-26 @ 9:15 AM
Study NCT ID:
NCT00154518
Status:
COMPLETED
Last Update Posted:
2013-02-22
First Post:
2005-09-09
Brief Title:
The Role of Matrix Metalloproteinases MMPs in Orthodontic Tooth Movement
Sponsor:
National Taiwan University Hospital
Organization:
National Taiwan University Hospital
Study Overview
Official Title:
The Role of Matrix Metalloproteinases in Orthodontic Treatment
Status:
COMPLETED
Status Verified Date:
2013-02
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
The purpose of this study is to test whether the two MMPs can be up-regulated during orthodontic treatment Alveolar bone samples will be collected from partially impacted third molars after orthodontic uprighting for different periods of time in volunteers
Detailed Description:
Long treatment time is a major factor causing high fees for orthodontic treatment Patients would have dental caries or periodontitis resulting from improper oral hygiene care during this long treatment period How to speed up the tooth movement which determines the duration of orthodontic treatment can help more people to obtain good occlusion and esthetics Orthodontic force on a tooth induces bone resorption on the compression side and bone deposition on the tension side thus the bone remodels and then the tooth moves Therefore bone resorption is the rate-limiting step of a lengthy orthodontic treatment
Bone resorption is a complex process The mineral component is dissolved by acid from osteoclasts On the other hand the organic components are digested with proteolytic enzymes secreted from osteoblasts and osteoclasts We focus on our study on specific proteases which can digest extracellular matrix called matrix metalloproteinases MMPs Osteoblast-derived MMPs play an important role during initiation of bone resorption However the mechanism of its regulation is not clear The past studies applied stretching or tension on single layer of cultured cells to characterize cellular response to the mechanical stimulation Now we simulate part of the bone resorption process by cultivating osteocyte-like cells in three-dimensional collagen gel under periodical compression
In a preliminary study we focus on transcriptional changes of MMPs upon compression in an osteosarcoma cell line MG-63 Initial data form microarray indicated specific increase of two MMPs expression after one day of compression This increased expression was specific because the levels of house-keeping genes ex Beta-actin or GAPDH and bone-specific markers were unaltered Therefore we proposed that increased MMP expression of osteoblasts under compression is the first step for bone remodeling switching from synthesis to degradation of osteoid In order to test this hypothesis the following specific aims will be achieved
1 To test whether these two MMPs can be up-regulated during orthodontic treatment Alveolar bone samples will be collected from partially impacted third molars after orthodontic uprighting for different periods of time in volunteers In situ hybridization and immunohistochemistry analysis for MMPs will reveal their roles in this physiological process
2 To optimize the regulation by changing the magnitude and frequency of the pressure and characterize the time table for these changes
Bone resorption is a complex process The mineral component is dissolved by acid from osteoclasts On the other hand the organic components are digested with proteolytic enzymes secreted from osteoblasts and osteoclasts We focus on our study on specific proteases which can digest extracellular matrix called matrix metalloproteinases MMPs Osteoblast-derived MMPs play an important role during initiation of bone resorption However the mechanism of its regulation is not clear The past studies applied stretching or tension on single layer of cultured cells to characterize cellular response to the mechanical stimulation Now we simulate part of the bone resorption process by cultivating osteocyte-like cells in three-dimensional collagen gel under periodical compression
In a preliminary study we focus on transcriptional changes of MMPs upon compression in an osteosarcoma cell line MG-63 Initial data form microarray indicated specific increase of two MMPs expression after one day of compression This increased expression was specific because the levels of house-keeping genes ex Beta-actin or GAPDH and bone-specific markers were unaltered Therefore we proposed that increased MMP expression of osteoblasts under compression is the first step for bone remodeling switching from synthesis to degradation of osteoid In order to test this hypothesis the following specific aims will be achieved
1 To test whether these two MMPs can be up-regulated during orthodontic treatment Alveolar bone samples will be collected from partially impacted third molars after orthodontic uprighting for different periods of time in volunteers In situ hybridization and immunohistochemistry analysis for MMPs will reveal their roles in this physiological process
2 To optimize the regulation by changing the magnitude and frequency of the pressure and characterize the time table for these changes
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None