Ignite Creation Date:
2024-05-06 @ 12:29 AM
Last Modification Date:
2024-10-26 @ 10:50 AM
Study NCT ID:
NCT01584219
Status:
UNKNOWN
Last Update Posted:
2012-05-08
First Post:
2012-04-23
Brief Title:
The Role of Placental Myeloid Cells During Gestation Labor and Disease
Sponsor:
Hillel Yaffe Medical Center
Organization:
Hillel Yaffe Medical Center
Study Overview
Official Title:
The Role of Placental Myeloid Cells During Gestation Labor and Disease
Status:
UNKNOWN
Status Verified Date:
2012-04
Last Known Status:
NOT_YET_RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Immature myeloid cells IMCs that are generated in the bone marrow and differentiate into mature granulocytes macrophages and dendritic cells DCs in the steady state Recently it was demonstrated that the IMC population expands in malignancy both in animal models and in humans These cells were described as immunosuppressants but have also been shown to promote tumor angiogenesis Accordingly IMCs were also found to take part in the burn injury wound healing process and other pathologies that involve angiogenesis It was shown in the investigators laboratory that a very similar population of IMCs populates the mouse and human placenta and that these cells actively promote angiogenesis
Dendritic cells DCs that can differentiate from IMCs are antigen presenting cells APCs that initiate and coordinate the innate and adaptive immune responses DCs can take up a diverse array of antigens and present them to T cells as peptides bound to MHC products These antigen-specific responses are critical for resistance to infection and tumors Conversely DCs have roles in autoimmunity transplant rejection and immunological tolerance In the reproductive system DCs were shown to account for 5-10 of all hematopoietic cells in the uterine decidua at the embryonic implantation site They were shown to promote angiogenesis during early pregnancy especially during implantation Very little is known about their function in the placenta and in the latter part of pregnancy when significant angiogenesis takes part
The investigators preliminary mouse experiments and human data demonstrate a shift in IMCDC populations with the development of the placenta The investigators hypothesize that this population shift may contribute to the labor and delivery process
The investigators aim is to understand the role of these myeloid cell populations during pregnancy to characterize their phenotype and try to shed light on the cellular and molecular mechanisms of pregnancy complications such as preeclampsia pre-term labor intrauterine growth restriction etc
Dendritic cells DCs that can differentiate from IMCs are antigen presenting cells APCs that initiate and coordinate the innate and adaptive immune responses DCs can take up a diverse array of antigens and present them to T cells as peptides bound to MHC products These antigen-specific responses are critical for resistance to infection and tumors Conversely DCs have roles in autoimmunity transplant rejection and immunological tolerance In the reproductive system DCs were shown to account for 5-10 of all hematopoietic cells in the uterine decidua at the embryonic implantation site They were shown to promote angiogenesis during early pregnancy especially during implantation Very little is known about their function in the placenta and in the latter part of pregnancy when significant angiogenesis takes part
The investigators preliminary mouse experiments and human data demonstrate a shift in IMCDC populations with the development of the placenta The investigators hypothesize that this population shift may contribute to the labor and delivery process
The investigators aim is to understand the role of these myeloid cell populations during pregnancy to characterize their phenotype and try to shed light on the cellular and molecular mechanisms of pregnancy complications such as preeclampsia pre-term labor intrauterine growth restriction etc
Detailed Description:
Identification of proangiogenic myeloid cell populations in human placentas Placental myeloid cells from different stages of pregnancy will be analyzed using flow cytometry
Aim 2 Characterization of the physiology of myeloid cells identified Cells will be isolated cultured and tested for proangiogenic properties using Matrigel plug assays endothelial tube formation assays etc Immune tolerance will be analyzed in vitro using T cell proliferation studies cytokine production etc
Aim 3 The role of placental myeloid cell populations in pathologies of pregnancy
Placentas from human pregnancy pathologies that involve placental dysfunction such as preeclampsia pre-term labor intrauterine growth restriction will be analyzed using the methods mentioned above
Aim 2 Characterization of the physiology of myeloid cells identified Cells will be isolated cultured and tested for proangiogenic properties using Matrigel plug assays endothelial tube formation assays etc Immune tolerance will be analyzed in vitro using T cell proliferation studies cytokine production etc
Aim 3 The role of placental myeloid cell populations in pathologies of pregnancy
Placentas from human pregnancy pathologies that involve placental dysfunction such as preeclampsia pre-term labor intrauterine growth restriction will be analyzed using the methods mentioned above
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None