Ignite Creation Date:
2024-05-05 @ 11:56 PM
Last Modification Date:
2024-10-26 @ 10:42 AM
Study NCT ID:
NCT01452971
Status:
UNKNOWN
Last Update Posted:
2011-10-17
First Post:
2011-10-13
Brief Title:
A Study of the Interaction Between Tumor Susceptibility Gene Glycine N-methyltransferase GNMT and Lung Cancer
Sponsor:
China Medical University Hospital
Organization:
China Medical University Hospital
Study Overview
Official Title:
A Study of the Interaction Between Tumor Susceptibility Gene GNMT and Lung Cancer
Status:
UNKNOWN
Status Verified Date:
2011-10
Last Known Status:
NOT_YET_RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Environmental carcinogens such as polycyclic aromatic hydrocarbons PAHs were reviewed as the major risk factors for lung cancer development In this proposal the investigators collected fifteen kinds of major PAHs and the investigators would like to perform the following studies
1 Study the gene expression and subcellular localization of GNMT in the normal-tumor tissue pairs of lung cancer patients
2 Study the associations of the polymorphisms of GNMT in lung cancer patients and the susceptibility to lung cancer
3 To assess the allelic loss at GNMT and determined the LOH rate of GNMT in the normal-tumor tissue pairs of lung cancer patients
4 Study the associations of the copy number variation CNV of GNMT and the susceptibility to lung cancer
5 Study the interaction between GNMT and polycyclic aromatic hydrocarbons PAHs in lung cancer cell lines
1 Study the gene expression and subcellular localization of GNMT in the normal-tumor tissue pairs of lung cancer patients
2 Study the associations of the polymorphisms of GNMT in lung cancer patients and the susceptibility to lung cancer
3 To assess the allelic loss at GNMT and determined the LOH rate of GNMT in the normal-tumor tissue pairs of lung cancer patients
4 Study the associations of the copy number variation CNV of GNMT and the susceptibility to lung cancer
5 Study the interaction between GNMT and polycyclic aromatic hydrocarbons PAHs in lung cancer cell lines
Detailed Description:
Multi-functional protein Glycine N-methyltransferase GNMT EC21120 localized to chromosome 6p12 affects genetic stability by a regulating the ratio of S-adenosylmethionine SAM to S-adenosylhomocystine SAH and b binding to folate Based on the following observations GNMT is classified as a tumor susceptibility gene a diminished GNMT expression levels in both human hepatocellular carcinoma HCC cell lines and tissues b genotypic analyses of several human GNMT gene polymorphisms showed a loss of heterozygosity in 36-47 of the genetic markers in HCC tissues and also related to the susceptibility of prostate cancer c GNMT binds to environmental carcinogens such as benzoapyrene BaP and aflatoxin B1 AFB1 and protect the liver cells by inhibiting DNA-adduct formation
Environmental carcinogens such as polycyclic aromatic hydrocarbons PAHs were reviewed as the major risk factors for lung cancer development In this proposal the investigators collected fifteen kinds of major PAHs and would like to perform the following studies
1 Study the gene expression and subcellular localization of GNMT in the normal-tumor tissue pairs of lung cancer patients
2 Study the associations of the polymorphisms of GNMT in lung cancer patients and the susceptibility to lung cancer
3 To assess the allelic loss at GNMT and determined the LOH rate of GNMT in the normal-tumor tissue pairs of lung cancer patients
4 Study the associations of the copy number variation CNV of GNMT and the susceptibility to lung cancer
5 Study the interaction between GNMT and polycyclic aromatic hydrocarbons PAHs in lung cancer cell lines
So far the investigators have analyzed the gene expression of GNMT in the normal-tumor tissue pairs of some lung cancer patients The investigators also analyzed the interaction between the GNMT and some PAHs using lung cancer cell lines These data are helpful to clarify the susceptibility of lung cancer patients with the gene expression in GNMT
This study is helpful in elucidating the role of GNMT plays in the tumorigenesis of lung cancer which may have important implications in the development of strategies for the prevention diagnosis and treatment of lung cancer and other types of cancer
Environmental carcinogens such as polycyclic aromatic hydrocarbons PAHs were reviewed as the major risk factors for lung cancer development In this proposal the investigators collected fifteen kinds of major PAHs and would like to perform the following studies
1 Study the gene expression and subcellular localization of GNMT in the normal-tumor tissue pairs of lung cancer patients
2 Study the associations of the polymorphisms of GNMT in lung cancer patients and the susceptibility to lung cancer
3 To assess the allelic loss at GNMT and determined the LOH rate of GNMT in the normal-tumor tissue pairs of lung cancer patients
4 Study the associations of the copy number variation CNV of GNMT and the susceptibility to lung cancer
5 Study the interaction between GNMT and polycyclic aromatic hydrocarbons PAHs in lung cancer cell lines
So far the investigators have analyzed the gene expression of GNMT in the normal-tumor tissue pairs of some lung cancer patients The investigators also analyzed the interaction between the GNMT and some PAHs using lung cancer cell lines These data are helpful to clarify the susceptibility of lung cancer patients with the gene expression in GNMT
This study is helpful in elucidating the role of GNMT plays in the tumorigenesis of lung cancer which may have important implications in the development of strategies for the prevention diagnosis and treatment of lung cancer and other types of cancer
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None