Ignite Creation Date:
2024-05-05 @ 11:56 PM
Last Modification Date:
2024-10-26 @ 10:42 AM
Study NCT ID:
NCT01450852
Status:
COMPLETED
Last Update Posted:
2011-10-12
First Post:
2011-10-03
Brief Title:
Strength Training Induced Alterations in Markers of Immune Function
Sponsor:
Louisiana State University Health Sciences Center in New Orleans
Organization:
Louisiana State University Health Sciences Center in New Orleans
Study Overview
Official Title:
Strength Training Induced Alterations in Markers of Immune Function
Status:
COMPLETED
Status Verified Date:
2011-10
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Exercise has been used to help prevent or slow the progression of inflammation-related disease however the mechanism by which this activity may lower concentrations of inflammatory markers remains unclear The melanocortin receptors 13 and 5 MC1R MC3R and MC5R have been shown to function in an anti-inflammatory manner and have the potential to mediate the positive immune adaptations associated with regular physical activity
Preliminary data suggest that MC3R gene expression increases in whole blood after chronic exercise training The primary aim of the current study is to explore whether this change in gene expression translates into alterations in MC1R MC3R or MC5R monocyte surface expression The secondary aim is to examine the relationship between surface expression of these receptors and circulating inflammatory profiles
The investigators will recruit 42 untrained healthy males and females aged 18-35 yrs Half of the group will be placed on an exercise program for 15 weeks The other half will serve as untrained control subjects In addition to basic anthropometric measures the investigators will measure concentrations of inflammatory and anti-inflammatory cytokines ELISA and cell surface expression of MC1R MC3R and MC5R on monocytes flow cytometry
Preliminary data suggest that MC3R gene expression increases in whole blood after chronic exercise training The primary aim of the current study is to explore whether this change in gene expression translates into alterations in MC1R MC3R or MC5R monocyte surface expression The secondary aim is to examine the relationship between surface expression of these receptors and circulating inflammatory profiles
The investigators will recruit 42 untrained healthy males and females aged 18-35 yrs Half of the group will be placed on an exercise program for 15 weeks The other half will serve as untrained control subjects In addition to basic anthropometric measures the investigators will measure concentrations of inflammatory and anti-inflammatory cytokines ELISA and cell surface expression of MC1R MC3R and MC5R on monocytes flow cytometry
Detailed Description:
Abstract
Chronic exercise reduces inflammation but the mechanisms involved are unclear Recent studies have shown that stimulation of melanocortin 1 and 3 receptors MC1R and MC3R on immune cells increases anti-inflammatory cytokine production PURPOSE To examine the influence of 12 weeks of resistance training RT on body composition monocyte cell-surface expression of MC1R and MC3R and circulating markers of inflammation METHODS Healthy active males and females age 20-27 yr were recruited into a RT group RE n 23 and an active control group AC n 19 RE completed 12 weeks of progressive periodized RT 3dwk while AC maintained normal activity habits Measures of body composition DXA were taken and blood was collected prior to PRE and following the intervention period POST Blood samples were analyzed for monocyte cell-surface expression MC1R MC3R MC5R and C-reactive protein CRP and the plasma cytokines interleukin 6 IL-6 and interleukin 10 IL-10 using flow cytometry and ELISAs respectively
Chronic exercise reduces inflammation but the mechanisms involved are unclear Recent studies have shown that stimulation of melanocortin 1 and 3 receptors MC1R and MC3R on immune cells increases anti-inflammatory cytokine production PURPOSE To examine the influence of 12 weeks of resistance training RT on body composition monocyte cell-surface expression of MC1R and MC3R and circulating markers of inflammation METHODS Healthy active males and females age 20-27 yr were recruited into a RT group RE n 23 and an active control group AC n 19 RE completed 12 weeks of progressive periodized RT 3dwk while AC maintained normal activity habits Measures of body composition DXA were taken and blood was collected prior to PRE and following the intervention period POST Blood samples were analyzed for monocyte cell-surface expression MC1R MC3R MC5R and C-reactive protein CRP and the plasma cytokines interleukin 6 IL-6 and interleukin 10 IL-10 using flow cytometry and ELISAs respectively
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None