Ignite Creation Date:
2024-05-05 @ 11:36 AM
Last Modification Date:
2024-10-26 @ 9:11 AM
Study NCT ID:
NCT00098072
Status:
COMPLETED
Last Update Posted:
2024-06-13
First Post:
2004-12-02
Brief Title:
Endothelial Cell Dysfunction in Pulmonary Hypertension
Sponsor:
National Institutes of Health Clinical Center CC
Organization:
National Institutes of Health Clinical Center CC
Study Overview
Official Title:
Endothelial Cell Dysfunction in Pulmonary Arterial Hypertension Biomarkers Mechanisms of Disease and Novel Therapeutic Targets
Status:
COMPLETED
Status Verified Date:
2024-04-25
Last Known Status:
None
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
This study will examine and test healthy volunteers and patients with pulmonary hypertension to try to learn more about the disease and find better ways to detect treat and if possible slow progression Pulmonary hypertension is a rare blood vessel disorder of the lung in which the pressure in the pulmonary artery the blood vessel that leads from the heart to the lungs rises above normal levels and may become life-threatening
Normal volunteers and patients with pulmonary hypertension 18 years of age and older may be eligible for this study All candidates are screened with a review of their medical records Normal volunteers also have a medical history electrocardiogram echocardiogram heart ultrasound and pulmonary function test in which the subject breathes in and out of a tube that measures lung volume mechanics and function
All participants undergo the following tests and procedures
Echocardiogram to measure heart function and blood pressure in the lungs A small probe held against the chest uses sound waves to obtain pictures of the heart
Magnetic resonance imaging MRI to evaluate the hearts pumping action Subjects lie on a stretcher that slides into a long tube-shaped scanner The machine uses a magnetic field and radio waves to obtain images of the heart
6-minute walk to measure how far the subject can walk in 6 minutes Subjects walk around the hospital for 6 minutes at a comfortable pace
Exercise testing to measure the ability to exercise and the subjects oxygen levels during exercise Subjects exercise on a bike or treadmill while the oxygen and carbon dioxide they breathe are measured using a small device placed in the mouth
Right heart catheterization to measure pressure in the heart and lungs A small catheter plastic tube is placed in an arm vein A longer catheter called a central line is placed in a deeper vein in the neck or just below the neck or in the leg or arm A long thin catheter that measures blood pressure directly is then inserted into the vein and advanced through the chambers of the heart into the lung artery to measure all the pressures in the heart and obtain blood samples
Genetic and protein studies DNA RNA and proteins from blood samples are studied for genes and proteins that might predict the development or progression of pulmonary hypertension
In addition to the above patients whose pulmonary hypertension was caused by a blood vessel injury undergo the tests described below The right heart catheter inserted for the catheterization procedure remains in place to obtain measurements of the effects of nitric oxide and nitrite in the following procedures
Inhalation of nitric oxide a gas naturally produced by cells lining arteries at 30-minute intervals to examine its effect on lung and heart pressures
Inhalation of aerosolized nitrite at 5-minute intervals to measure its effects on lung and heart pressures
Inhalation of nitric oxide for up to 24 hours to obtain multiple measurements of its effect on lung and heart pressures
Blood draws for laboratory tests
In patients whose pulmonary hypertension was caused by a blood vessel injury we also plan to follow response to standard therapy After the initiation of standard therapy we will restudy the same parameters excluding NO and sodium nitrite studies in these patients at approximately 4 months and yearly for 5 years
Normal volunteers and patients with pulmonary hypertension 18 years of age and older may be eligible for this study All candidates are screened with a review of their medical records Normal volunteers also have a medical history electrocardiogram echocardiogram heart ultrasound and pulmonary function test in which the subject breathes in and out of a tube that measures lung volume mechanics and function
All participants undergo the following tests and procedures
Echocardiogram to measure heart function and blood pressure in the lungs A small probe held against the chest uses sound waves to obtain pictures of the heart
Magnetic resonance imaging MRI to evaluate the hearts pumping action Subjects lie on a stretcher that slides into a long tube-shaped scanner The machine uses a magnetic field and radio waves to obtain images of the heart
6-minute walk to measure how far the subject can walk in 6 minutes Subjects walk around the hospital for 6 minutes at a comfortable pace
Exercise testing to measure the ability to exercise and the subjects oxygen levels during exercise Subjects exercise on a bike or treadmill while the oxygen and carbon dioxide they breathe are measured using a small device placed in the mouth
Right heart catheterization to measure pressure in the heart and lungs A small catheter plastic tube is placed in an arm vein A longer catheter called a central line is placed in a deeper vein in the neck or just below the neck or in the leg or arm A long thin catheter that measures blood pressure directly is then inserted into the vein and advanced through the chambers of the heart into the lung artery to measure all the pressures in the heart and obtain blood samples
Genetic and protein studies DNA RNA and proteins from blood samples are studied for genes and proteins that might predict the development or progression of pulmonary hypertension
In addition to the above patients whose pulmonary hypertension was caused by a blood vessel injury undergo the tests described below The right heart catheter inserted for the catheterization procedure remains in place to obtain measurements of the effects of nitric oxide and nitrite in the following procedures
Inhalation of nitric oxide a gas naturally produced by cells lining arteries at 30-minute intervals to examine its effect on lung and heart pressures
Inhalation of aerosolized nitrite at 5-minute intervals to measure its effects on lung and heart pressures
Inhalation of nitric oxide for up to 24 hours to obtain multiple measurements of its effect on lung and heart pressures
Blood draws for laboratory tests
In patients whose pulmonary hypertension was caused by a blood vessel injury we also plan to follow response to standard therapy After the initiation of standard therapy we will restudy the same parameters excluding NO and sodium nitrite studies in these patients at approximately 4 months and yearly for 5 years
Detailed Description:
Introduction Primary pulmonary hypertension now known as idiopathic pulmonary arterial hypertension IPAH a subgroup of pulmonary arterial hypertension PAH is a rare disorder characterized by severe morbidity and high mortality rates There are no routine screening tests or validated markers of disease activity in IPAH or the broader group of PAH Therefore patients usually present at advanced stages of disease The pathogenesis of IPAH and other forms of PAH remain unclear Prior theories stressed a one-hit hypothesis Current thinking focuses on a two-hit hypothesis 1 genetic susceptibility and 2 a triggering stimulus that initiates pulmonary vascular injury resulting in endothelial cell EC dysfunction and the mobilization of endothelial progenitor cells EPC Loss of function mutations in the bone morphogenetic protein receptor 2 BMPR2 gene has been implicated in the pathogenesis of IPAH EC dysfunction in IPAH has been associated with decreases in both endothelial nitric oxide synthase eNOS expression and nitric oxide NO production Peripheral blood mononuclear cells PBMCs interact with an altered endothelial cell surface which may also be important in the disease process
Objectives We plan to define a subset of differentially regulated biomarkers in IPAH and PAH that may lead to earlier diagnosis and better methods for measuring responses to therapy Specifically we hope to identify biomarkers of IPAH and other forms of PAH that is suggestive of NO therapeutic response and which may be useful in titrating NO therapy We also hope to identify novel targets for the development of new therapeutic strategies
Methods This study will consist of a pilot study and a primary study The pilot study will enroll up to 30 patients and 30 controls in order to obtain completed studies on 10 normal subjects and 10 patients with PAH The goal of the pilot study is to determine the best technique for circulating endothelial cell CEC and PBMC identification quantification and isolation and EPC identification and quantification The subjects in the pilot phase undergo right heart catheterization to obtain hemodynamics and pulmonary artery blood Pulmonary artery and peripheral blood will be used for EPC quantification and CEC and PBMC isolation CECs and PBMCs will be studied in depth using high density oligonucleotide microarrays In addition plasma obtained from PAH patients and healthy volunteers will be applied in vitro to various cell populations suspected to be central to disease pathogenesis including but not limited to ECs circulating mononuclear cells cardiac myocytes andor vascular smooth muscle cells Phenotypic alterations induced by plasma exposure will be assessed using in vitro assays
The primary study will recruit the following subject groups 1 patients with IPAH and other forms of PAH vascular injury-induced pulmonary hypertension who currently are on no therapy less than or equal to 6 months of IV therapy or less than or equal to one year of oral therapy 2 patients with pulmonary hypertension PH ascribed to a nonvascular injury process and 3 normal individuals The following baseline studies will be performed in all groups 1 noninvasive assessment of right ventricular RV function by echocardiogram and magnetic resonance imaging MRI 2 determination of exercise capacity by cardiopulmonary stress test and six minute walk 3 measurement of hemodynamic parameters by right heart catheterization and 4 characterization of disease phenotype by cell surface markers oligonucleotide microarrays and proteomics using peripheral and pulmonary arterial blood EPCs will be quantitated and CECs and PBMCs will be isolated and analyzed by flow cytometry for expression of cell surface markers involved in coagulation adhesion and angiogenesis as these are important processes in IPAH and PAH Furthermore ECs identified by positive and negative selection and isolated by cell sorting and PBMCs will be studied in depth using high density oligonucleotide microarrays to more fully characterize their transcriptome
A major impediment to the widespread use of chronic home based inhaled NO are related to its delivery system and duration of effects In PAH patients we plan to study a novel NO delivery system INO pulse delivery device Patients with PAH will be given inhaled NO 20 and 40 ppm and then placed on inhaled NO using the INO pulse delivery device for 24 hours Hemodynamics will be obtained serially with each dose and upon completion of 24 hours of therapy pulmonary artery and peripheral blood will be drawn and reexamined by flow cytometry microarrays and proteomics
We also plan to follow response to standard therapy as determined by the referring physician After the initial day 0 studies we will restudy the same parameters excluding NO studies in patients with PAH at approximately 4 months and yearly for 5 years after therapeutic intervention
Objectives We plan to define a subset of differentially regulated biomarkers in IPAH and PAH that may lead to earlier diagnosis and better methods for measuring responses to therapy Specifically we hope to identify biomarkers of IPAH and other forms of PAH that is suggestive of NO therapeutic response and which may be useful in titrating NO therapy We also hope to identify novel targets for the development of new therapeutic strategies
Methods This study will consist of a pilot study and a primary study The pilot study will enroll up to 30 patients and 30 controls in order to obtain completed studies on 10 normal subjects and 10 patients with PAH The goal of the pilot study is to determine the best technique for circulating endothelial cell CEC and PBMC identification quantification and isolation and EPC identification and quantification The subjects in the pilot phase undergo right heart catheterization to obtain hemodynamics and pulmonary artery blood Pulmonary artery and peripheral blood will be used for EPC quantification and CEC and PBMC isolation CECs and PBMCs will be studied in depth using high density oligonucleotide microarrays In addition plasma obtained from PAH patients and healthy volunteers will be applied in vitro to various cell populations suspected to be central to disease pathogenesis including but not limited to ECs circulating mononuclear cells cardiac myocytes andor vascular smooth muscle cells Phenotypic alterations induced by plasma exposure will be assessed using in vitro assays
The primary study will recruit the following subject groups 1 patients with IPAH and other forms of PAH vascular injury-induced pulmonary hypertension who currently are on no therapy less than or equal to 6 months of IV therapy or less than or equal to one year of oral therapy 2 patients with pulmonary hypertension PH ascribed to a nonvascular injury process and 3 normal individuals The following baseline studies will be performed in all groups 1 noninvasive assessment of right ventricular RV function by echocardiogram and magnetic resonance imaging MRI 2 determination of exercise capacity by cardiopulmonary stress test and six minute walk 3 measurement of hemodynamic parameters by right heart catheterization and 4 characterization of disease phenotype by cell surface markers oligonucleotide microarrays and proteomics using peripheral and pulmonary arterial blood EPCs will be quantitated and CECs and PBMCs will be isolated and analyzed by flow cytometry for expression of cell surface markers involved in coagulation adhesion and angiogenesis as these are important processes in IPAH and PAH Furthermore ECs identified by positive and negative selection and isolated by cell sorting and PBMCs will be studied in depth using high density oligonucleotide microarrays to more fully characterize their transcriptome
A major impediment to the widespread use of chronic home based inhaled NO are related to its delivery system and duration of effects In PAH patients we plan to study a novel NO delivery system INO pulse delivery device Patients with PAH will be given inhaled NO 20 and 40 ppm and then placed on inhaled NO using the INO pulse delivery device for 24 hours Hemodynamics will be obtained serially with each dose and upon completion of 24 hours of therapy pulmonary artery and peripheral blood will be drawn and reexamined by flow cytometry microarrays and proteomics
We also plan to follow response to standard therapy as determined by the referring physician After the initial day 0 studies we will restudy the same parameters excluding NO studies in patients with PAH at approximately 4 months and yearly for 5 years after therapeutic intervention
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
False
Is a FDA Regulated Device?:
False
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None
Secondary IDs
| Secondary ID | Type | Domain | Link |
|---|---|---|---|
| 05-CC-0041 | None | None | None |