Ignite Creation Date:
2024-05-05 @ 11:15 PM
Last Modification Date:
2024-10-26 @ 10:30 AM
Study NCT ID:
NCT01287468
Status:
UNKNOWN
Last Update Posted:
2011-02-01
First Post:
2011-01-28
Brief Title:
Academia Sinica Investigator Award 2010
Sponsor:
Taipei Medical University WanFang Hospital
Organization:
Taipei Medical University WanFang Hospital
Study Overview
Official Title:
Academia Sinica Investigator Award 2010
Status:
UNKNOWN
Status Verified Date:
2011-01
Last Known Status:
ACTIVE_NOT_RECRUITING
Delayed Posting:
No
If Stopped, Why?:
Not Stopped
Has Expanded Access:
False
If Expanded Access, NCT#:
N/A
Has Expanded Access, NCT# Status:
N/A
Acronym:
None
Brief Summary:
Over the past five years a spectrum of studies has shown that the stromal cell populations surrounding or embedding cancer cells in a variety of different tumor types are intrinsically involved in the mechanisms affecting tumor growth and metastasis Among these cells fibroblasts and myeloid derived suppressor cells MDSCs have been shown to play key roles in the regulation of tumor cell behaviors via differential gene expression regulation and secretion of specific chemokines eg RANTES CXCL12 and CXCL14 cytokines eg TNF-α IL-1β NF-κB and control by inflammatory and immunomodulatory cells molecules or potent growth factors Of particular interest is that the various stromal immune cells dendritic cells and T cells and inflammatory MDSCs found in primary and metastatic tumors behave very differently from those present in normal or non-cancerous counterpart tissues In light of these new understandings in cancer biology the investigators believe that systematic and innovative approaches to further research are very much needed in Taiwan and at our Academy Here the investigators propose to investigate the tumor suppressive effect of anti-inflammatory phytomedicines on regulation of stromal immune cells and fibroblasts in breast cancers The investigators will employ the TSA and 4T1 mouse mammary carcinoma system to conduct an in vivo study of several specific candidate phytomedicines cytopiloyne Wedelia chinensis shikonin emodin and others that the investigators have already identified as conferring anti-inflammation related activities for inhibition of TSA and 4T1 tumor growth and specific effects on inflammation-associated cellular and molecular functions of stromal immune cells fibroblasts and MDSCs When potent and specific anti-tumor effects are detected the investigators will extend our study to a three-dimensional collagenMatrigel culture system for ex-vivo study focusing on the stromal cell-mediated or -associated anti-tumor effects of TSA or 4T1 cells using the organoid tissue culture systems the investigators have previously developed in our laboratory JNCI 1979 Cancer Res 1981 The investigators will establish reconstituted TSA or 4T1 tumor cells with the stromal immune cells in a co-cultivation system When the investigators have achieved demonstrable success the investigators will extend the mouse system to human breast cancers including primary and metastatic tumors by a close collaboration which the investigators have already established with Drs
Detailed Description:
Ji-Hong Jhu 朱紀洪 Hospital President MDsurgeon and Huey-Kong Sytwu 司徒惠康 MD PhD at the National Defense University Hospital The investigators will then study the molecular signaling effect of specific phytocompounds already identified to confer in vivo mouse anti-tumor and anti-inflammatory activities in primaryorganoid cultures of human breast tumor cells under ex vivo conditions Finally the investigators intend to identify the key molecular regulators immune-modulatory effectors of test stromal immune cells that are affected by the candidate phytomedicines using molecular and cell biology assays to investigate the interplay between immune cells and tumor cells in the stromal tissues
Our study is expected to reveal one or more highly specific anti-inflammatory phytocompounds that mechanistically confer specific effects on stromal fibroblast or immune cell types resulting in potent anti-tumor effects on test mammary carcinoma systems Five Specific Aims are thus designed to investigate the immuno-modulating effects of specific medicinal constituents from TCM materials The experimental findings obtained from this study would provide insights in the molecular cellular and signaling mechanismseffects of several medicinal phytocompounds that may have potential applications in chemoprevention or herbal medicine adjuvant treatment of human breast cancers
Our study is expected to reveal one or more highly specific anti-inflammatory phytocompounds that mechanistically confer specific effects on stromal fibroblast or immune cell types resulting in potent anti-tumor effects on test mammary carcinoma systems Five Specific Aims are thus designed to investigate the immuno-modulating effects of specific medicinal constituents from TCM materials The experimental findings obtained from this study would provide insights in the molecular cellular and signaling mechanismseffects of several medicinal phytocompounds that may have potential applications in chemoprevention or herbal medicine adjuvant treatment of human breast cancers
Study Oversight
Has Oversight DMC:
None
Is a FDA Regulated Drug?:
None
Is a FDA Regulated Device?:
None
Is an Unapproved Device?:
None
Is a PPSD?:
None
Is a US Export?:
None
Is an FDA AA801 Violation?:
None