Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 5:11 AM
Last Modification Date:
2025-12-25 @ 5:11 AM
Retention:
SAMPLES_WITH_DNA
Description:
The purpose of the epigenetic substudy is to collecting blood is for exploratory research into epigenetic effects of salmeterol therapy. Blood was collected at Visit 2 and Visit 3.
Steps:
* Obtain informed consent
* Label vacutainer tube with patient's study ID number, visit number, date of collection (use a sharpee pen)
* Collect 10mL blood sample into purple top tube before starting methacholine challenge (must be done before any albuterol is given at the visit)
* Immediately place tube in crushed ice or in refrigerator
* Spin tube; freeze tube with cells for future genotyping/methylation studies; divide plasma as equally as possible between the 3 cryotubes, freeze the EDTA tube and 3 cryotubes.
Steps:
* Obtain informed consent
* Label vacutainer tube with patient's study ID number, visit number, date of collection (use a sharpee pen)
* Collect 10mL blood sample into purple top tube before starting methacholine challenge (must be done before any albuterol is given at the visit)
* Immediately place tube in crushed ice or in refrigerator
* Spin tube; freeze tube with cells for future genotyping/methylation studies; divide plasma as equally as possible between the 3 cryotubes, freeze the EDTA tube and 3 cryotubes.
Section:
The purpose of the epigenetic substudy is to collecting blood is for exploratory research into epigenetic effects of salmeterol therapy. Blood was collected at Visit 2 and Visit 3.
Steps:
* Obtain informed consent
* Label vacutainer tube with patient's study ID number, visit number, date of collection (use a sharpee pen)
* Collect 10mL blood sample into purple top tube before starting methacholine challenge (must be done before any albuterol is given at the visit)
* Immediately place tube in crushed ice or in refrigerator
* Spin tube; freeze tube with cells for future genotyping/methylation studies; divide plasma as equally as possible between the 3 cryotubes, freeze the EDTA tube and 3 cryotubes.