Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 3:21 AM
Last Modification Date:
2025-12-25 @ 3:21 AM
Retention:
SAMPLES_WITH_DNA
Description:
At birth, cord blood samples (200-500 µl) will be collected and subjected to:
a. Measurement of LINE1 promoter methylation. Genomic DNA extraction, LINE1 methylation and bioinformatic analysis as in Fontana et al. 2021.
The first meconium will be collected into a sterile tube and suspended in phosphate-buffered saline at a concentration of 100 g/L. Samples will be filtered and stored at -80°C until use:
b1. Stool metabolomic analysis will be performed as described in Roggero et al. 2020. Two-microlitre of samples will be processed through the gas chromatography-mass spectrometry system.
b2. Secretome analysis: a large number of soluble molecules (cytokines/chemokines, growth factors, and other inflammation-related molecules) will be measured using xMAP technology on the Luminex platform. Commercial kits (Bio-Rad Laboratories and/or R\&D Systems) will be used. Results will be confirmed with ELISA or other immunometric analyses.
a. Measurement of LINE1 promoter methylation. Genomic DNA extraction, LINE1 methylation and bioinformatic analysis as in Fontana et al. 2021.
The first meconium will be collected into a sterile tube and suspended in phosphate-buffered saline at a concentration of 100 g/L. Samples will be filtered and stored at -80°C until use:
b1. Stool metabolomic analysis will be performed as described in Roggero et al. 2020. Two-microlitre of samples will be processed through the gas chromatography-mass spectrometry system.
b2. Secretome analysis: a large number of soluble molecules (cytokines/chemokines, growth factors, and other inflammation-related molecules) will be measured using xMAP technology on the Luminex platform. Commercial kits (Bio-Rad Laboratories and/or R\&D Systems) will be used. Results will be confirmed with ELISA or other immunometric analyses.
Section:
At birth, cord blood samples (200-500 µl) will be collected and subjected to:
a. Measurement of LINE1 promoter methylation. Genomic DNA extraction, LINE1 methylation and bioinformatic analysis as in Fontana et al. 2021.
The first meconium will be collected into a sterile tube and suspended in phosphate-buffered saline at a concentration of 100 g/L. Samples will be filtered and stored at -80°C until use:
b1. Stool metabolomic analysis will be performed as described in Roggero et al. 2020. Two-microlitre of samples will be processed through the gas chromatography-mass spectrometry system.
b2. Secretome analysis: a large number of soluble molecules (cytokines/chemokines, growth factors, and other inflammation-related molecules) will be measured using xMAP technology on the Luminex platform. Commercial kits (Bio-Rad Laboratories and/or R\&D Systems) will be used. Results will be confirmed with ELISA or other immunometric analyses.