Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 2:42 AM
Last Modification Date:
2025-12-25 @ 2:42 AM
Retention:
SAMPLES_WITH_DNA
Description:
Types of Specimens:
Formalin-fixed, paraffin-embedded (FFPE) tumor tissue obtained from surgical resection.
Peripheral blood samples for germline DNA extraction.
Time of Collection:
Tumor tissue will be collected intraoperatively.
Blood samples will be collected postoperatively at the time of referral for genetic testing.
Purpose of Analysis:
Tumor tissue will undergo IHC for MMR protein expression.
Based on IHC results, additional analyses may include BRAF mutation and MLH1 promoter methylation.
Blood-derived germline DNA will be analyzed via next-generation sequencing (NGS) to detect pathogenic variants associated with Lynch syndrome.
Storage and Handling:
FFPE blocks will be stored in hospital pathology units.
Blood samples for DNA extraction will be processed and stored according to standard protocols in certified genetic laboratories.
Location:
Specimens will be collected from multiple surgical centers across Türkiye.
Formalin-fixed, paraffin-embedded (FFPE) tumor tissue obtained from surgical resection.
Peripheral blood samples for germline DNA extraction.
Time of Collection:
Tumor tissue will be collected intraoperatively.
Blood samples will be collected postoperatively at the time of referral for genetic testing.
Purpose of Analysis:
Tumor tissue will undergo IHC for MMR protein expression.
Based on IHC results, additional analyses may include BRAF mutation and MLH1 promoter methylation.
Blood-derived germline DNA will be analyzed via next-generation sequencing (NGS) to detect pathogenic variants associated with Lynch syndrome.
Storage and Handling:
FFPE blocks will be stored in hospital pathology units.
Blood samples for DNA extraction will be processed and stored according to standard protocols in certified genetic laboratories.
Location:
Specimens will be collected from multiple surgical centers across Türkiye.
Section:
Types of Specimens:
Formalin-fixed, paraffin-embedded (FFPE) tumor tissue obtained from surgical resection.
Peripheral blood samples for germline DNA extraction.
Time of Collection:
Tumor tissue will be collected intraoperatively.
Blood samples will be collected postoperatively at the time of referral for genetic testing.
Purpose of Analysis:
Tumor tissue will undergo IHC for MMR protein expression.
Based on IHC results, additional analyses may include BRAF mutation and MLH1 promoter methylation.
Blood-derived germline DNA will be analyzed via next-generation sequencing (NGS) to detect pathogenic variants associated with Lynch syndrome.
Storage and Handling:
FFPE blocks will be stored in hospital pathology units.
Blood samples for DNA extraction will be processed and stored according to standard protocols in certified genetic laboratories.
Location:
Specimens will be collected from multiple surgical centers across Türkiye.