Bio Spec Overview
Ignite Creation Date:
2026-03-26 @ 3:15 PM
Last Modification Date:
2026-03-26 @ 3:15 PM
Retention:
SAMPLES_WITH_DNA
Description:
The study involved the collection of human blood samples, specifically processed into the following formats:
Serum: Obtained by collecting blood in clean tubes, allowing it to clot, and centrifuging at 1000 rpm for 10 minutes.
Plasma/Supernatant: Collected into EDTA-containing tubes and centrifuged to separate the supernatant.
Total RNA: Extracted from the blood samples, specifically including the microRNA fraction for PCR analysis.
Storage and Laboratory Processing
Serum Storage: Serum samples used for alpha-fetoprotein (AFP) ELISA estimation were kept frozen at -20 °C.
RNA Storage: The supernatant for RNA extraction was stored in RNase-free tubes at -80 °C prior to the extraction process.
Molecular Analysis: The retained specimens were used for reverse transcription of cDNA and subsequent Real-time RT-qPCR to determine the relative number of Mir copies using the ΔΔCT method.
Serum: Obtained by collecting blood in clean tubes, allowing it to clot, and centrifuging at 1000 rpm for 10 minutes.
Plasma/Supernatant: Collected into EDTA-containing tubes and centrifuged to separate the supernatant.
Total RNA: Extracted from the blood samples, specifically including the microRNA fraction for PCR analysis.
Storage and Laboratory Processing
Serum Storage: Serum samples used for alpha-fetoprotein (AFP) ELISA estimation were kept frozen at -20 °C.
RNA Storage: The supernatant for RNA extraction was stored in RNase-free tubes at -80 °C prior to the extraction process.
Molecular Analysis: The retained specimens were used for reverse transcription of cDNA and subsequent Real-time RT-qPCR to determine the relative number of Mir copies using the ΔΔCT method.
Section:
The study involved the collection of human blood samples, specifically processed into the following formats:
Serum: Obtained by collecting blood in clean tubes, allowing it to clot, and centrifuging at 1000 rpm for 10 minutes.
Plasma/Supernatant: Collected into EDTA-containing tubes and centrifuged to separate the supernatant.
Total RNA: Extracted from the blood samples, specifically including the microRNA fraction for PCR analysis.
Storage and Laboratory Processing
Serum Storage: Serum samples used for alpha-fetoprotein (AFP) ELISA estimation were kept frozen at -20 °C.
RNA Storage: The supernatant for RNA extraction was stored in RNase-free tubes at -80 °C prior to the extraction process.
Molecular Analysis: The retained specimens were used for reverse transcription of cDNA and subsequent Real-time RT-qPCR to determine the relative number of Mir copies using the ΔΔCT method.