Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 2:08 AM
Last Modification Date:
2025-12-25 @ 2:08 AM
Retention:
SAMPLES_WITH_DNA
Description:
The preserved biological samples comprised fresh human fecal specimens and human tongue coating samples.
Microbial DNA will be extracted from fecal and tongue coating samples using the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany). Whole-genome DNA was isolated via the centrifugal adsorption column method, and DNA quality was evaluated by agarose gel electrophoresis (1.0% agarose concentration, 150V voltage, 20-minute electrophoresis time). Samples meeting the following criteria-distinct genomic bands, total DNA quantity \>200 ng, DNA concentration \>10 ng/μL, OD260/280 \>1, and OD260/230 \>2-were selected for sequencing library construction and subsequent sequencing.
Microbial DNA will be extracted from fecal and tongue coating samples using the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany). Whole-genome DNA was isolated via the centrifugal adsorption column method, and DNA quality was evaluated by agarose gel electrophoresis (1.0% agarose concentration, 150V voltage, 20-minute electrophoresis time). Samples meeting the following criteria-distinct genomic bands, total DNA quantity \>200 ng, DNA concentration \>10 ng/μL, OD260/280 \>1, and OD260/230 \>2-were selected for sequencing library construction and subsequent sequencing.
Section:
The preserved biological samples comprised fresh human fecal specimens and human tongue coating samples.
Microbial DNA will be extracted from fecal and tongue coating samples using the QIAamp DNA Microbiome Kit (Qiagen, Hilden, Germany). Whole-genome DNA was isolated via the centrifugal adsorption column method, and DNA quality was evaluated by agarose gel electrophoresis (1.0% agarose concentration, 150V voltage, 20-minute electrophoresis time). Samples meeting the following criteria-distinct genomic bands, total DNA quantity \>200 ng, DNA concentration \>10 ng/μL, OD260/280 \>1, and OD260/230 \>2-were selected for sequencing library construction and subsequent sequencing.