Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 12:19 AM
Last Modification Date:
2025-12-25 @ 12:19 AM
Retention:
SAMPLES_WITH_DNA
Description:
Seperation of human neutrophils were according to a standardized procedures and following steps: using 3%(w/v) dextran sedimentation for 30 min, then gradient centrifugation in Ficoll-Hypaque, and hypotonic lysis of erythrocytes. The purified neutrophils were determined by the Trypan blue exclusion method and need to contained \>98% viable cells. Then they were suspended in without calcium ion HBSS buffer (pH 7.4), and stored at 4 °C for analysis
Section:
Seperation of human neutrophils were according to a standardized procedures and following steps: using 3%(w/v) dextran sedimentation for 30 min, then gradient centrifugation in Ficoll-Hypaque, and hypotonic lysis of erythrocytes. The purified neutrophils were determined by the Trypan blue exclusion method and need to contained \>98% viable cells. Then they were suspended in without calcium ion HBSS buffer (pH 7.4), and stored at 4 °C for analysis