Bio Spec Overview
Ignite Creation Date:
2025-12-25 @ 12:18 AM
Last Modification Date:
2025-12-25 @ 12:18 AM
Retention:
SAMPLES_WITH_DNA
Description:
One or two 50 mL urine tubes and 17.5 mL blood tubes are collected. The urine cell pellet is recovered from each sample by centrifugation and lysed according to a well-defined protocol established by the manufacturer and then stored at -80°C.
* The PaXgen tube is left at room temperature for 2 hours according to the protocol and then stored at -20°C.
* Serum is recovered after centrifugation of the dry tube at 4000 rpm for 10 minutes and stored at -80°C.
* PBMC are extracted from the Cpt tube, frozen at 50% in SVF-10% DMSO and stored at -80°C.
Depending on the study, mRNA expression is then analysed by quantitative PCR (targeted research) or microarray.
* The PaXgen tube is left at room temperature for 2 hours according to the protocol and then stored at -20°C.
* Serum is recovered after centrifugation of the dry tube at 4000 rpm for 10 minutes and stored at -80°C.
* PBMC are extracted from the Cpt tube, frozen at 50% in SVF-10% DMSO and stored at -80°C.
Depending on the study, mRNA expression is then analysed by quantitative PCR (targeted research) or microarray.
Section:
One or two 50 mL urine tubes and 17.5 mL blood tubes are collected. The urine cell pellet is recovered from each sample by centrifugation and lysed according to a well-defined protocol established by the manufacturer and then stored at -80°C.
* The PaXgen tube is left at room temperature for 2 hours according to the protocol and then stored at -20°C.
* Serum is recovered after centrifugation of the dry tube at 4000 rpm for 10 minutes and stored at -80°C.
* PBMC are extracted from the Cpt tube, frozen at 50% in SVF-10% DMSO and stored at -80°C.
Depending on the study, mRNA expression is then analysed by quantitative PCR (targeted research) or microarray.