Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 11:52 PM
Last Modification Date:
2025-12-24 @ 11:52 PM
Retention:
SAMPLES_WITH_DNA
Description:
Blood will be collected for serum/plasma during baseline and 6 \& 12 month follow-up Adipose tissue (omentum, visceral) will be collected during surgery. Venous blood samples (20mL = 4 teaspoons) will be collected prior to surgery, 6 month and 1 year postoperatively. The plasma samples will be obtained by centrifugation at 800 g for 15 min, and the aliquots will be transferred to polypropylene tubes and stored at -80°C until they are assayed. Total RNA and miRNA will be isolated according to manufacturer instructions.
The first morning urine samples (minimum 30mL) will be collected. Frozen tubes will be used and stored at -80 °C until processing for Nuclear Magnetic Resonance (NMR) analysis.
The first morning urine samples (minimum 30mL) will be collected. Frozen tubes will be used and stored at -80 °C until processing for Nuclear Magnetic Resonance (NMR) analysis.
Section:
Blood will be collected for serum/plasma during baseline and 6 \& 12 month follow-up Adipose tissue (omentum, visceral) will be collected during surgery. Venous blood samples (20mL = 4 teaspoons) will be collected prior to surgery, 6 month and 1 year postoperatively. The plasma samples will be obtained by centrifugation at 800 g for 15 min, and the aliquots will be transferred to polypropylene tubes and stored at -80°C until they are assayed. Total RNA and miRNA will be isolated according to manufacturer instructions.
The first morning urine samples (minimum 30mL) will be collected. Frozen tubes will be used and stored at -80 °C until processing for Nuclear Magnetic Resonance (NMR) analysis.