Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 11:39 PM
Last Modification Date:
2025-12-24 @ 11:39 PM
Retention:
SAMPLES_WITH_DNA
Description:
The screening analysis will be performed on blood samples available in European Institute of Oncology Biobank by Next Generation Sequencing (NGS) technology using the TruSight Hereditary Cancer panel (Illumina) to analyze the entire coding regions of 113 genes selected genes and 125 SNPs, starting from 50 ng of gDNA extracted with MagCore HF16 Plus (Diatech Labline).
Libraries will be constructed following the manufacturer protocols (Nextera Flex for Enrichment Illumina) and will be sequenced with Illumina MiSeq Sequencer or Illumina NextSeq Sequencer available in the laboratory.
Data will be analyzed with a dedicated software. Finally, all mutations will be confirmed using the Sanger sequencing method (3500xL Dx Genetic Analyzer, Thermo Fisher Scientific).
Libraries will be constructed following the manufacturer protocols (Nextera Flex for Enrichment Illumina) and will be sequenced with Illumina MiSeq Sequencer or Illumina NextSeq Sequencer available in the laboratory.
Data will be analyzed with a dedicated software. Finally, all mutations will be confirmed using the Sanger sequencing method (3500xL Dx Genetic Analyzer, Thermo Fisher Scientific).
Section:
The screening analysis will be performed on blood samples available in European Institute of Oncology Biobank by Next Generation Sequencing (NGS) technology using the TruSight Hereditary Cancer panel (Illumina) to analyze the entire coding regions of 113 genes selected genes and 125 SNPs, starting from 50 ng of gDNA extracted with MagCore HF16 Plus (Diatech Labline).
Libraries will be constructed following the manufacturer protocols (Nextera Flex for Enrichment Illumina) and will be sequenced with Illumina MiSeq Sequencer or Illumina NextSeq Sequencer available in the laboratory.
Data will be analyzed with a dedicated software. Finally, all mutations will be confirmed using the Sanger sequencing method (3500xL Dx Genetic Analyzer, Thermo Fisher Scientific).