Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 11:38 PM
Last Modification Date:
2025-12-24 @ 11:38 PM
Retention:
SAMPLES_WITH_DNA
Description:
Explant liver with be sent for assessment of the subcapsular layer in addition to routine Histo pathological examination (HPE).
20 ml of peritoneal fluid from the recipient will also be collected and processed for analysis of peritoneal macrophages.
Samples will then be subjected to flow cytometry for assessment of:
1. Macrophage identification markers: Quantitative assessment .
2. Macrophage Activation markers: Qualitative/ Activation assessment. Density and functionality of both LCM and PM will then be co-related to both pre-LT sepsis timeline and profile as well as post operative recipient outcome .
Identification of Macrophage populations using Flow Cytometry and Immunohistochemistry
20 ml of peritoneal fluid from the recipient will also be collected and processed for analysis of peritoneal macrophages.
Samples will then be subjected to flow cytometry for assessment of:
1. Macrophage identification markers: Quantitative assessment .
2. Macrophage Activation markers: Qualitative/ Activation assessment. Density and functionality of both LCM and PM will then be co-related to both pre-LT sepsis timeline and profile as well as post operative recipient outcome .
Identification of Macrophage populations using Flow Cytometry and Immunohistochemistry
Section:
Explant liver with be sent for assessment of the subcapsular layer in addition to routine Histo pathological examination (HPE).
20 ml of peritoneal fluid from the recipient will also be collected and processed for analysis of peritoneal macrophages.
Samples will then be subjected to flow cytometry for assessment of:
1. Macrophage identification markers: Quantitative assessment .
2. Macrophage Activation markers: Qualitative/ Activation assessment. Density and functionality of both LCM and PM will then be co-related to both pre-LT sepsis timeline and profile as well as post operative recipient outcome .
Identification of Macrophage populations using Flow Cytometry and Immunohistochemistry