Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 11:19 PM
Last Modification Date:
2025-12-24 @ 11:19 PM
Retention:
SAMPLES_WITH_DNA
Description:
Human DNA will be extracted from tumor tissues using the FFPE DNA Tissue Kit (Qiagen, Hilden, Germany), following the manufacturer's instructions. DNA quality will be checked using NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA). Genomic DNA (500ng) will be converted by sodium bisulfite with the EZ DNA Methylation Gold Kit (Zymo Research, Irvine, CA, USA) and eluted in 20μL RNase free water, according to the manufacturer's instructions.
Section:
Human DNA will be extracted from tumor tissues using the FFPE DNA Tissue Kit (Qiagen, Hilden, Germany), following the manufacturer's instructions. DNA quality will be checked using NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA). Genomic DNA (500ng) will be converted by sodium bisulfite with the EZ DNA Methylation Gold Kit (Zymo Research, Irvine, CA, USA) and eluted in 20μL RNase free water, according to the manufacturer's instructions.