Bio Spec Overview
Ignite Creation Date:
2025-12-24 @ 10:54 PM
Last Modification Date:
2025-12-24 @ 10:54 PM
Retention:
SAMPLES_WITH_DNA
Description:
For each subject, total RNA was extracted from serum samples using the TRIzol LS reagent (Invitrogen, Carlsbad, CA), and subjected to RT-PCR analysis. A ready-made solutions containing the primers and probes for human miR-146a and miR-125ba (Applied Biosystems, Foster City, CA) and real-time RT-PCR was estimated using an ABI 7300 system (Applied Bio systems) (Mazloom et al.,2015).
Section:
For each subject, total RNA was extracted from serum samples using the TRIzol LS reagent (Invitrogen, Carlsbad, CA), and subjected to RT-PCR analysis. A ready-made solutions containing the primers and probes for human miR-146a and miR-125ba (Applied Biosystems, Foster City, CA) and real-time RT-PCR was estimated using an ABI 7300 system (Applied Bio systems) (Mazloom et al.,2015).