Bio Spec

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Overview

Bio Spec Overview

Ignite Creation Date: 2025-12-24 @ 10:52 PM
Last Modification Date: 2025-12-24 @ 10:52 PM
Retention: SAMPLES_WITH_DNA
Description: * Histological sample at inclusion. The following analysis will be performed: usual staining techniques to evaluate the presence of portal inflammation, interface hepatitis and fibrosis. Expression of group of genes involved in liver rejection (CXCL9, CXCL10, FoxP3, TK1, CD74, MMP9) will be evaluated with qPCR in RNA of the tissue samples cryopreserved at -80ºC. Immunochemical staining to quantity the number of lymphocytes CD4+, CD8+ and Tregs.
* Blood samples collected at the inclusion and in every follow-up visit:

* Frequency and characteristics of Tregs (CD4+, CD25+, CD127- and FoxP3). Intracellular stain of CTLA-4 will be evaluated as well
* Stimulation with IL2 to study Tregs response.
* Faecal sample at inclusion to evaluate microbiome.
Section: * Histological sample at inclusion. The following analysis will be performed: usual staining techniques to evaluate the presence of portal inflammation, interface hepatitis and fibrosis. Expression of group of genes involved in liver rejection (CXCL9, CXCL10, FoxP3, TK1, CD74, MMP9) will be evaluated with qPCR in RNA of the tissue samples cryopreserved at -80ºC. Immunochemical staining to quantity the number of lymphocytes CD4+, CD8+ and Tregs. * Blood samples collected at the inclusion and in every follow-up visit: * Frequency and characteristics of Tregs (CD4+, CD25+, CD127- and FoxP3). Intracellular stain of CTLA-4 will be evaluated as well * Stimulation with IL2 to study Tregs response. * Faecal sample at inclusion to evaluate microbiome.